LOCUS       sequence-215085-        1199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Arai S, Kriszt R, Harada K, Looi LS, Matsuda S, Wongso D, Suo S, 
            Ishiura S, Tseng YH, Raghunath M, Ito T, Tsuboi T, Kitaguchi T
  TITLE     RGB-color intensiometric indicators visualize spatiotemporal 
            dynamics of ATP in single cells.
  JOURNAL   Angew Chem Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304.
  PUBMED    29952110
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304."
COMMENT     Sequence Label: MaLionG
FEATURES             Location/Qualifiers
     source          1..1199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             919..1170
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1107..1128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctggctagc gtttaaacgg gccctctaga ctcgagatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      241 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctggcgcgga
      481 atgaagaccg tgaaggtaaa catcaccacc cccgacggcc ccgtgtacga cgccgacatc
      541 gagatggtga gcgtgcgggc cgagagcggc gacctgggca tcctgcccgg ccacatcccc
      601 accaaggccc ccctgaagat cggcgccgtg cggctgaaga aggacggcca gaccgagtac
      661 gtggccgtga gcggcggcaa cgtggaggtg cggcccgacc aggtgaccat caccgcccag
      721 gccgccgaga ccgccgaggg catcgacaag gagcgggccg aggccgcccg gcagcgggcc
      781 caggagcggc tgaacagcca gagcgacgac accgacatcc ggcgggccga gctggccctg
      841 cagcgggccc tgaaccggct ggacgtggcc gagatgaagg cactgaagga attcagccac
      901 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      961 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctgtacaag taattcgaag cttaagttta aaccgctga
//
