LOCUS       sequence-215086-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  negative control for Green ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Arai S, Kriszt R, Harada K, Looi LS, Matsuda S, Wongso D, Suo S, 
            Ishiura S, Tseng YH, Raghunath M, Ito T, Tsuboi T, Kitaguchi T
  TITLE     RGB-color intensiometric indicators visualize spatiotemporal 
            dynamics of ATP in single cells.
  JOURNAL   Angew Chem Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304.
  PUBMED    29952110
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304."
COMMENT     Sequence Label: negMaLionG
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(172..193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(433..452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1009..1260
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1197..1218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1294..1311)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga
      121 ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      181 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      361 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 gggcacaagc tggagtacaa cccgcgtgga atgaagaccg tgaaggtaaa catcaccacc
      601 cccgacggcc ccgtgtacga cgccgacatc gagatggtga gcgtgcgggc cgagagcggc
      661 gacctgggca tcctgcccgg ccacatcccc accaaggccc ccctgaagat cggcgccgtg
      721 cggctgaaga aggacggcca gaccgagtac gtggccgtga gcggcggcaa cgtggaggtg
      781 cggcccgacc aggtgaccat caccgcccag gccgccgaga ccgccgaggg catcgacaag
      841 gagcgggccg aggccgcccg gcagcgggcc caggagcggc tgaacagcca gagcgacgac
      901 accgacatcc ggcgggccga gctggccctg cagcgggccc tgaaccggct ggacgtggcc
      961 gagatgaagg cacctggtga attcagccac aacgtctata tcatggccga caagcagaag
     1021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1141 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1261 taattcgaag cttaagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc
//
