LOCUS       sequence-215088-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  negative control for Red ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Arai S, Kriszt R, Harada K, Looi LS, Matsuda S, Wongso D, Suo S, 
            Ishiura S, Tseng YH, Raghunath M, Ito T, Tsuboi T, Kitaguchi T
  TITLE     RGB-color intensiometric indicators visualize spatiotemporal 
            dynamics of ATP in single cells.
  JOURNAL   Angew Chem Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304.
  PUBMED    29952110
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304."
COMMENT     Sequence Label: negMaLionR
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     549..568
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1290..1307)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtttaaacgg
       61 gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc accacactgg
      121 actagtggat ccatgctggt gagcaagggc gaggaggata acatggccat catcaaggag
      181 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      241 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag
      301 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      361 gcctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc
      421 ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt taaccaggac
      481 tcctccctgc aggacggcgt attcatctac aaggtgaagc tgcgcggcac caacttcccc
      541 cccgacggcc ccgtaatgca gaagaagacc atgggctggg agccttctcg cgggatgaag
      601 accgtgaagg tgaacatcac cacccccgac ggccccgtgt acgacgccga catcgagatg
      661 gtgagcgtgc gggccgagag cggcgacctg ggcatcctgc ccggccacat ccccaccaag
      721 gcccccctga agatcggcgc cgtgcggctg aagaaggacg gccagaccga gtacgtggcc
      781 gtgagcggcg gcaacgtgga ggtgcggccc gaccaggtga ccatcaccgc ccaggccgcc
      841 gagaccgccg agggcatcga caaggagcgg gccgaggccg cccggcagcg ggcccaggag
      901 cggctgaaca gccagagcga cgacaccgac atccggcggg ccgagctggc cctgcagcgg
      961 gccctgaacc ggctggacgt ggccgagatg aagcctgaag gtgtttccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc
     1081 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
     1201 gaacagtgcg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 taaaagctta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
//
