LOCUS       sequence-215090-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  negative control for Blue ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Arai S, Kriszt R, Harada K, Looi LS, Matsuda S, Wongso D, Suo S, 
            Ishiura S, Tseng YH, Raghunath M, Ito T, Tsuboi T, Kitaguchi T
  TITLE     RGB-color intensiometric indicators visualize spatiotemporal 
            dynamics of ATP in single cells.
  JOURNAL   Angew Chem Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304.
  PUBMED    29952110
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2018 Jun 27. doi: 10.1002/anie.201804304."
COMMENT     Sequence Label: negMaLionB
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(451..470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1212..1233
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
       61 gcgtttaaac gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt
      121 ccaccacact ggactagtgg atccatggtg agcaagggcg aggagctgtt caccggggtg
      181 gtgcccatcc aggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      241 gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      301 aagctgcccg tgccctggcc caccctcgtg accaccctgt cacacggcgt gcagtgcttc
      361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccggaggc
      421 tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      541 gaggacggca acatcctggg gcacaagctg gagtacaacc tccgcaatat gaagaccgtg
      601 aaggtgaaca tcaccacccc cgacggcccc gtgtacgacg ccgacatcga gatggtgagc
      661 gtgcgggccg agagcggcga cctgggcatc ctgcccggcc acatccccac caaggccccc
      721 ctgaagatcg gcgccgtgcg gctgaagaag gacggccaga ccgagtacgt ggccgtgagc
      781 ggcggcaacg tggaggtgcg gcccgaccag gtgaccatca ccgcccaggc cgccgagacc
      841 gccgagggca tcgacaagga gcgggccgag gccgcccggc agcgggccca ggagcggctg
      901 aacagccaga gcgacgacac cgacatccgg cgggccgagc tggccctgca gcgggccctg
      961 aaccggctgg acgtggccga gatgaaggca gaattctcac cagagaacgt ctatatcaag
     1021 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgagggc
     1081 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1141 ctgctgcccg acaaccacta cctgagcgtg cagtccatac tttcgaaaga ccccaacgag
     1201 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1261 gacgagctgt acaagtaaaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
//
