LOCUS       sequence-215100-       13797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding the cytosolic localization of the FRET 
            semi-synthetic biosensor measuring the ratio NADPH/NADP+ in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
  TITLE     Semisynthetic biosensors for mapping cellular concentrations of 
            nicotinamide adenine dinucleotides.
  JOURNAL   eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
  PUBMED    29809136
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-05-29"; volume: "7. pii: 32638. doi"; pages: " 
            10.7554/eLife.32638"
COMMENT     Sequence Label: pEBTet_NADP-Snifit-cyto
FEATURES             Location/Qualifiers
     source          1..13797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             807..1691
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     primer_bind     complement(2422..2439)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2428..2539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3080..4869
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7533..7551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7619..7723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7724..7792,7793..8584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7942..7961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8755..9343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9244..9263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(9532..9552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9550..9847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9924..10523
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9924..9943)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10420..10440
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10614..10748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10886..10905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11182..11443
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          11560..12132
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     12140..12159
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     12187..12206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12187..12205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12216..12836
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(12289..12308)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    12878..12999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        13054..13433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13434..13637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13588..13608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    13639..13657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13660..13678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     13688..13712
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agcttaccac catggagggc gggctggggc gtgctgtgtg cttgctgacc ggggcctccc
       61 gcggcttcgg ccggacgctg gccccgctcc tggcctcgct gctgtcgccc ggctccgtgc
      121 ttgtccttag cgcccgcaac gacgaggcac tgcgccagct ggaggccgag ctgggcgccg
      181 agcggtctgg cctgcgcgtg gtgcgggtgc ccgccgacct gggcgccgag gccggcttgc
      241 agcagctgct cggcgccctg cgcgagctcc cccggcccaa ggggctgcag cgactgctgc
      301 ttatcaacaa cgcgggctct cttggggatg tgtccaaagg cttcgtggac ctgagtgact
      361 ccactcaagt gaacaactac tgggcactga acttgacctc catgctctgc ctgacttcca
      421 gcgtcctgaa ggccttcccg gacagtcctg gcctcaacag aaccgtggtt aacatctcgt
      481 ccctctgtgc cctgcaacct ttcaaaggct gggcgctgta ctgtgcagga aaggctgctc
      541 gtgatatgct gttccaggtc ctggcgctgg aggaacctaa tgtgagggtg ctgaactatg
      601 ccccaggtcc tctggacaca gacatgcagc agttggcccg ggagacctcc gtggacccag
      661 acatgcgaaa agggctgcag gagctgaagg caaaggggaa gctggtggat tgcaaggtgt
      721 cagcccagaa actgctgagc ttactggaaa aggacgagtt caagtctgga gcccacgtgg
      781 acttctatga caaagaattc ggatccgaaa tcggtactgg ctttccattc gacccccatt
      841 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
      901 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
      961 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
     1021 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
     1081 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
     1141 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
     1201 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
     1261 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
     1321 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
     1381 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
     1441 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
     1501 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
     1561 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
     1621 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
     1681 agatttccgg tagcggccgc cctcctcctc cgccaccgcc accaccgccg ccgccgccac
     1741 caccacctcc tccgcctcct ccaccaccac cgccaccgcc tccaccacct ggcggccgct
     1801 ctcggtctct cgagatggat aaagactgcg aaatgaaacg cactaccctg gacagcccgc
     1861 tgggtaaact ggaactgagc ggttgcgagc agggcctgca cgaaatcatt ttcctgggca
     1921 aaggtacgag cgctgcggat gcggtcgaag taccggctcc ggctgcggtt ctgggtggcc
     1981 cggagccgct gatgcaggct accgcatggc tgaacgcata ttttcaccag ccagaggcga
     2041 ttgaagagtt ccctgtaccg gccctgcatc acccggtatt tcagcaggaa tcctttaccc
     2101 gtcaggtact gtggaaactg ctgaaagtag tgaaattcgg cgaggtgatc tcctattccc
     2161 atctggcggc gctggctggc aacccagcgg caactgcggc ggtgaaaacc gctctgtctg
     2221 gtaatccggt gccaattctg atcccgtgcc accgtgtggt tcagggcgac ctggacgtag
     2281 gtggctacga aggcggcctg gctgtgaaag aatggctgct ggcgcacgaa ggtcaccgtc
     2341 tgggtaaacc aggcctgggc taaagctagc ggcgcgcccg gccgctcgag tctagagggc
     2401 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2461 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2521 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2581 tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag gatcgatccc
     2641 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     2701 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     2761 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     2821 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     2881 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     2941 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3001 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3061 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3121 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3181 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3241 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3301 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3361 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3421 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3481 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     3541 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     3601 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     3661 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     3721 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     3781 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     3841 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     3901 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     3961 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4021 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4081 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4141 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4201 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4261 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4321 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4381 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4441 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     4501 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4561 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4621 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     4681 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     4741 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
     4801 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
     4861 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
     4921 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
     4981 agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac
     5041 gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg
     5101 ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta
     5161 acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt
     5221 catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca
     5281 aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct
     5341 ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta
     5401 aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc
     5461 caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa
     5521 gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac
     5581 cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc
     5641 ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttt
     5701 ccaaaccacc ctcctttttt gcgccctgcc tccatcaccc tgaccccggg gtccagtgct
     5761 tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg
     5821 ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg
     5881 gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg
     5941 actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc
     6001 acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg
     6061 accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc
     6121 acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
     6181 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     6241 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     6301 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     6361 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     6421 ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     6481 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
     6541 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
     6601 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     6661 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     6721 tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     6781 tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc
     6841 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
     6901 atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg
     6961 tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc
     7021 ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc
     7081 tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga
     7141 cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat
     7201 ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat
     7261 cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat
     7321 ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta
     7381 gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta
     7441 ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc
     7501 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     7561 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     7981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     8281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8881 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9361 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
     9421 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     9481 cataatgggg aaggccatcc agcctcgcgt cgaactagat gatccggctg tggaatgtgt
     9541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9841 tttttggagg gtgaccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac
     9901 ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9961 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
    10021 cgcgccacac cgtcgacccc gaccgccaca tcgaacgcgt caccgagctg caagaactct
    10081 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
    10141 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
    10201 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    10261 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    10321 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    10381 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    10441 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    10501 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
    10561 gcgcacgacc cggtccgacg cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt
    10621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10741 tctggatctg atccgaaccc cttcctcgac caattctcat gtttgacagc ttatcatcgc
    10801 agatccgggc aacgttgttg ccattgctcg accaattctc atgtttgaca gcttatcatc
    10861 gcagatccgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10921 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    10981 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    11041 cgctgcttcg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta
    11101 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc
    11161 gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat
    11221 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
    11281 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
    11341 gattggacga accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga
    11401 tacaataaac gccatttgac cattcaccac attggtgtgc acctccaagc tggccgggga
    11461 tcctctaggg cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc
    11521 ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat
    11581 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
    11641 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
    11701 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
    11761 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
    11821 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
    11881 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
    11941 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
    12001 atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
    12061 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
    12121 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
    12181 gaattgtaat acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag
    12241 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
    12301 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
    12361 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
    12421 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
    12481 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
    12541 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
    12601 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
    12661 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
    12721 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
    12781 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt
    12841 acagcggatc ccgggaattc agatcttatt aaagcagaac ttgtttattg cagcttataa
    12901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    12961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgaatccatg
    13021 gcgcagaact ggtaggtatg gaagatccgc gttgacattg attattgact agttattaat
    13081 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
    13141 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
    13201 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
    13261 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
    13321 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
    13381 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
    13441 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
    13501 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
    13561 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
    13621 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
    13681 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
    13741 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaactta
//
