LOCUS       sequence-215138-       10772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin (chicken) tagged with VenusA206K at the C-terminus in 
            lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: pRRL-Vinculin-Venus
FEATURES             Location/Qualifiers
     source          1..10772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     543..562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     796..813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    930..951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(966..971,972..989,990..996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1004..1020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1009..1031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1028..1044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1028..1044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1063..1083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1065..1083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1111..1337
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1338..1518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1565..1690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2183..2416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2601..2645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2912..3028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3088..3467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3468..3671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3622..3642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3716..3734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(6977..6979,6980..6982,6983..7696)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7022..7043)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7283..7302)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7630..7651
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7722..8310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7775..7795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8193..8204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8401..8634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8706..8827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8743..8762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8797..8816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8867..9002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9023..9041)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9051..9068)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9051..9067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9060..9082)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9209..9664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9296..9315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9506..9527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9690..9794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9795..9863,9864..10655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10013..10032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
       61 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1021 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     1081 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     1141 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     1201 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     1261 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     1321 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1501 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1561 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1621 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1681 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1741 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1801 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1861 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1921 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1981 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     2041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2341 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2821 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2881 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     2941 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3001 aaaaacaaat tacaaaattc aaaattttat cgatggatcc cccgggctcg agttcgaaga
     3061 tcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3481 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3541 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3601 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3661 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     3721 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg ccatgcccgt
     3781 cttccacacg cgcaccatcg agagcatctt ggagcccgtg gctcagcaga tctcccacct
     3841 ggtcatcatg cacgaggagg gggaggtaga cggcaaggcc atcccggacc tcaccgcccc
     3901 cgtgtcggcc gtgcaggccg ctgtcagcaa cctggtgcgg gttggaaaag aaactgtgca
     3961 gacaacagaa gaccagatct tgaaaaggga tatgccacca gcattcatca aagtagagaa
     4021 tgcctgcacc aagctcgttc gagcagccca gatgctgcaa gcagatcctt attcagtacc
     4081 agctcgtgac tacctaattg atggatcaag aggcatcctt tctggaacat cagacttact
     4141 tctgacattt gatgaagcgg aggtccgtaa aatcatccgt gtctgcaaag gaatattgga
     4201 atatctgact gtggcagaag tagtagagac tatggaggat ttggtgacat atacaaagaa
     4261 tctagggcca ggaatgacaa agatggcgaa aatgatcgat gagagacaac aggaattaac
     4321 tcatcaggaa catagggtta tgctggtgaa ctccatgaat actgtgaagg agctattgcc
     4381 tgtacttatt tcagctatga agatctttgt aaccacaaaa aacactaaaa gccagggaat
     4441 agaagaggcc ttgaaaaatc gcaatttcac agtagagaaa atgagtgctg agataaatga
     4501 aataatccgt gtattacaac tcacttcctg ggatgaagat gcctgggcca gcaaggacac
     4561 tgaagccatg aaaagagctt tagccctaat agattcaaag atgaaccagg caaaaggctg
     4621 gctgagagat ccaaacgcac ctccagggga tgctggtgag caagcaataa ggcagatcct
     4681 tgatgaagct ggaaaagcag gagaattgtg tgcaggcaaa gaacgcaggg agattctggg
     4741 aacatgcaaa actttgggcc aaatgactga tcaacttgct gatctccgag ctagaggaca
     4801 gggtgctaca ccgatggcaa tgcagaaggc acagcaggtg tcacaaggcc tggatttgct
     4861 cactgcaaaa gtggagaatg cagcccggaa attggaggcc atgacaaact ctaagcaggc
     4921 tattgcaaag aagattgatg ctgctcagaa ctggcttgcg gatcccaacg gcggaagtga
     4981 aggagaagaa cacattcgag gaattatgtc tgaagcaagg aaagttgcag aattgtgtga
     5041 ggagcctaaa gaaagagatg atatccttcg ctccttgggg gaaatctctg ctctgacagc
     5101 taagctgtca gatctgcgac gacatgggaa aggcgactct cctgaggccc gtgcattggc
     5161 caagcaaata gctacatcac ttcagaactt acagtccaaa acaaacagag ctgtagcaaa
     5221 tactaggcca gttaaagctg ctgtccattt ggagggcaag attgagcaag cccagaggtg
     5281 gatagataat cctacagttg atgatcgggg agtaggccag gcagcaattc ggggtttggt
     5341 tgcagaaggt cgtcgtctag ccaatgtcat gatgggacct tatcgtcagg acctgcttgc
     5401 caaatgtgac cgtgtagacc agctggctgc tcagcttgct gaccttgcag caagagggga
     5461 aggagagtct cctcaggcta gggcaattgc tgctcagctt caggactccc tgaaggatct
     5521 caaagcacgg atgcaagaag caatgaccca ggaggtgtct gatgttttca gtgacactac
     5581 aacccctatt aagttgttag cagtagcagc cactgctcct tctgatactc ccaatagaga
     5641 agaggtgttt gaagaaagag cagcaaattt tgaaaaccat gctgctagac tgggagcaac
     5701 agcagaaaaa gcagctgcag ttggaactgc taataaaact actgtggaag gcattcaggc
     5761 aacagtcaaa tctgcaaggg agcttacacc acaggtagta tcggctgctc gaatcctcct
     5821 gagaaatcct ggaaatcaag ctgcttatga gcattttgag acaatgaaaa accaatggat
     5881 tgataatgta gaaaagatga cagggctggt ggatgaggcc atcgatacca agtctctgtt
     5941 ggatgcatca gaagaggcta ttaagaagga tcttgataaa tgtaaagttg caatggccaa
     6001 tatgcaacct cagatgctgg tagctggagc caccagcatt gctagacgag cgaaccgcat
     6061 cctgcttgtg gcaaaacggg aggttgaaaa ttcagaagac cctaaattca gggaggctgt
     6121 taaagcagct tctgatgagc tgagcaaaac catatcaccg atggtaatgg atgctaaagc
     6181 tgtagcagga aatatctctg atcctggttt gcagaagagt ttcttggatt ctggatacag
     6241 gattctggga gctgtggcca aagtcagaga agcctttcag cctcaggagc cagactttcc
     6301 ccctcctcct cctgacctag agcatctcca tctgactgat gagcttgctc ctccaaaacc
     6361 accacttcca gaaggtgagg ttcccccacc cagaccacca ccccctgaag aaaaagatga
     6421 ggagttccca gagcagaaag caggagaagc tattaatcag cccatgatga tggctgctag
     6481 gcagttgcat gacgaggctc ggaaatggtc tagcaagggt aacgacatca ttgctgctgc
     6541 taaacgaatg gcgctgctca tggctgagat gtcacgcctg gtgcgaggag gcagcggaaa
     6601 caagcgtgcc ctcatccagt gtgcaaaaga tattgctaag gcatcggatg aagtcactcg
     6661 attggccaaa gaggtggcca agcaatgtac tgataaacgc attagaacaa acctcttaca
     6721 ggtctgtgag cgaatcccaa ccatcagcac gcagctcaaa attctttcca cagtgaaagc
     6781 taccatgctg ggcaggacta acatcagcga tgaagaatca gaacaggcaa ctgagatgtt
     6841 ggttcataac gcccagaacc tcatgcagtc tgtgaaggaa actgtgagag aagctgaagc
     6901 agcatccatt aagataagaa cagatgccgg attcactctg cgctgggtca gaaagacccc
     6961 atggtatcag gaattcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7021 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7081 gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc
     7141 cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta
     7201 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7261 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7321 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7381 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc
     7441 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     7501 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7561 gctgcccgac aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa
     7621 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7681 cgagctgtac aagtaagcgg ccgctcgagt ctagagtcga caatcaacct ctggattaca
     7741 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7801 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7861 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7921 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7981 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8041 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8101 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     8161 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8221 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8281 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttcgagctcg gtacctttaa
     8341 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8401 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8641 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8701 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8761 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8821 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8941 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9001 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9061 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9181 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9241 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9301 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9361 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9421 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9481 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9541 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9601 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9661 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9781 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9841 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9901 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9961 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10021 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10081 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10141 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10201 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10261 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10321 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10381 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10441 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10501 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10561 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10621 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10681 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10741 ttgataatct catgaccaaa atcccttaac gt
//
