LOCUS       sequence-215140-       11635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor with point mutation affecting ability to 
            bind talin in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: pRRL-VinculinTS-A50I
FEATURES             Location/Qualifiers
     source          1..11635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..147
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        207..586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        587..790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     741..761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        835..853
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3547..4254
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4375..4377,4378..4380,4381..5091)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4420..4441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4681..4700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5028..5049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5704..6292
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5757..5777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6175..6186)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6383..6616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6688..6809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6725..6744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6779..6798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6849..6984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7005..7023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7033..7050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7033..7049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7042..7064)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7191..7646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7278..7297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7488..7509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7672..7776
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7777..7845,7846..8637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7995..8014)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8808..9396
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9297..9316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9550..9567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9684..9705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9720..9725,9726..9743,9744..9750)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9758..9774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9763..9785
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9782..9798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9782..9798
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9817..9837
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9819..9837
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9865..10091
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10092..10272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10319..10444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10937..11170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11355..11399
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta
       61 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      121 aaaacaaatt acaaaattca aaattttatc gatggatccc ccgggctcga gttcgaagat
      181 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      241 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      781 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      841 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc catgcccgtc
      901 ttccacacgc gcaccatcga gagcatcttg gagcccgtgg ctcagcagat ctcccacctg
      961 gtcatcatgc acgaggaggg ggaggtagac ggcaaggcca tcccggacct caccgccccc
     1021 gtgtcggccg tgcaggccat cgtcagcaac ctggtgcggg ttggaaaaga aactgtgcag
     1081 acaacagaag accagatctt gaaaagggat atgccaccag cattcatcaa agtagagaat
     1141 gcctgcacca agctcgttcg agcagcccag atgctgcaag cagatcctta ttcagtacca
     1201 gctcgtgact acctaattga tggatcaaga ggcatccttt ctggaacatc agacttactt
     1261 ctgacatttg atgaagcgga ggtccgtaaa atcatccgtg tctgcaaagg aatattggaa
     1321 tatctgactg tggcagaagt agtagagact atggaggatt tggtgacata tacaaagaat
     1381 ctagggccag gaatgacaaa gatggcgaaa atgatcgatg agagacaaca ggaattaact
     1441 catcaggaac atagggttat gctggtgaac tccatgaata ctgtgaagga gctattgcct
     1501 gtacttattt cagctatgaa gatctttgta accacaaaaa acactaaaag ccagggaata
     1561 gaagaggcct tgaaaaatcg caatttcaca gtagagaaaa tgagtgctga gataaatgaa
     1621 ataatccgtg tattacaact cacttcctgg gatgaagatg cctgggccag caaggacact
     1681 gaagccatga aaagagcttt agccctaata gattcaaaga tgaaccaggc aaaaggctgg
     1741 ctgagagatc caaacgcacc tccaggggat gctggtgagc aagcaataag gcagatcctt
     1801 gatgaagctg gaaaagcagg agaattgtgt gcaggcaaag aacgcaggga gattctggga
     1861 acatgcaaaa ctttgggcca aatgactgat caacttgctg atctccgagc tagaggacag
     1921 ggtgctacac cgatggcaat gcagaaggca cagcaggtgt cacaaggcct ggatttgctc
     1981 actgcaaaag tggagaatgc agcccggaaa ttggaggcca tgacaaactc taagcaggct
     2041 attgcaaaga agattgatgc tgctcagaac tggcttgcgg atcccaacgg cggaagtgaa
     2101 ggagaagaac acattcgagg aattatgtct gaagcaagga aagttgcaga attgtgtgag
     2161 gagcctaaag aaagagatga tatccttcgc tccttggggg aaatctctgc tctgacagct
     2221 aagctgtcag atctgcgacg acatgggaaa ggcgactctc ctgaggcccg tgcattggcc
     2281 aagcaaatag ctacatcact tcagaactta cagtccaaaa caaacagagc tgtagcaaat
     2341 actaggccag ttaaagctgc tgtccatttg gagggcaaga ttgagcaagc ccagaggtgg
     2401 atagataatc ctacagttga tgatcgggga gtaggccagg cagcaattcg gggtttggtt
     2461 gcagaaggtc gtcgtctagc caatgtcatg atgggacctt atcgtcagga cctgcttgcc
     2521 aaatgtgacc gtgtagacca gctggctgct cagcttgctg accttgcagc aagaggggaa
     2581 ggagagtctc ctcaggctag ggcaattgct gctcagcttc aggactccct gaaggatctc
     2641 aaagcacgga tgcaagaagc aatgacccag gaggtgtctg atgttttcag tgacactaca
     2701 acccctatta agttgttagc agtagcagcc actgctcctt ctgatactcc caatagagaa
     2761 gaggtgtttg aagaaagagc agcaaatttt gaaaaccatg ctgctagact gggagcaaca
     2821 gcagaaaaag cagctgcagt tggaactgct aataaaacta ctgtggaagg cattcaggca
     2881 acagtcaaat ctgcaaggga gcttacacca caggtagtat cggctgctcg aatcctcctg
     2941 agaaatcctg gaaatcaagc tgcttatgag cattttgaga caatgaaaaa ccaatggatt
     3001 gataatgtag aaaagatgac agggctggtg gatgaggcca tcgataccaa gtctctgttg
     3061 gatgcatcag aagaggctat taagaaggat cttgataaat gtaaagttgc aatggccaat
     3121 atgcaacctc agatgctggt agctggagcc accagcattg ctagacgagc gaaccgcatc
     3181 ctgcttgtgg caaaacggga ggttgaaaat tcagaagacc ctaaattcag ggaggctgtt
     3241 aaagcagctt ctgatgagct gagcaaaacc atatcaccga tggtaatgga tgctaaagct
     3301 gtagcaggaa atatctctga tcctggtttg cagaagagtt tcttggattc tggatacagg
     3361 attctgggag ctgtggccaa agtcagagaa gcctttcagc ctcaggagcc agactttccc
     3421 cctcctcctc ctgacctaga gcatctccat ctgactgatg agcttgctcc tccaaaacca
     3481 ccacttccag aaggtgaggt tcccccaccc agaccaccac cccctgaaga aaaagatgag
     3541 gtcgagatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag
     3601 atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc
     3661 gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc
     3721 ctgcccttct cctacgacat tctgaccacc gcgttcgcct acggcaacag ggccttcacc
     3781 aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg
     3841 gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg
     3901 gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc
     3961 cccgtgatgc agaagaagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc
     4021 gacggcgtgc tgaagggcga cgtcaagcac aagctgctgc tggagggcgg cggccaccac
     4081 cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc cgactatcac
     4141 tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa ggtgaccgtt
     4201 tacgagagcg ccgtggcccg caactccacc gacggcatgg acgagctgta caaggggcca
     4261 ggtggtgcag ggccaggtgg tgcagggcca ggtggtgcag ggccaggtgg tgcagggccc
     4321 ggtggtgcag gtccaggtgg tgcaggtcca ggtggtgcag gtccaggtgg tgctatggtg
     4381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4441 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4501 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4561 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4801 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     4861 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     4921 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4981 agctaccagt ccaaactgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5041 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggcggccgcg
     5101 gagttcccag agcagaaagc aggagaagct attaatcagc ccatgatgat ggctgctagg
     5161 cagttgcatg acgaggctcg gaaatggtct agcaagggta acgacatcat tgctgctgct
     5221 aaacgaatgg cgctgctcat ggctgagatg tcacgcctgg tgcgaggagg cagcggaaac
     5281 aagcgtgccc tcatccagtg tgcaaaagat attgctaagg catcggatga agtcactcga
     5341 ttggccaaag aggtggccaa gcaatgtact gataaacgca ttagaacaaa cctcttacag
     5401 gtctgtgagc gaatcccaac catcagcacg cagctcaaaa ttctttccac agtgaaagct
     5461 accatgctgg gcaggactaa catcagcgat gaagaatcag aacaggcaac tgagatgttg
     5521 gttcataacg cccagaacct catgcagtct gtgaaggaaa ctgtgagaga agctgaagca
     5581 gcatccatta agataagaac agatgccgga ttcactctgc gctgggtcag aaagacccca
     5641 tggtatcagt aagaattctg cagatatcca gcacagtggc ggccgctcga gtctagagtc
     5701 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5761 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5821 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5881 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5941 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6001 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6061 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     6121 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6181 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6241 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     6301 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6361 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6421 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6481 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6541 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6601 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6661 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6781 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6841 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6901 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6961 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     7021 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7081 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7141 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7321 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7441 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7501 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7561 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7621 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7681 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7741 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7801 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7861 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7921 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7981 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8041 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8101 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8161 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8221 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8281 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8341 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8401 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8461 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8521 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8581 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8641 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8701 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8941 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9541 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9601 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     9661 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9721 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9781 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     9841 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     9901 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     9961 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
    10021 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
    10081 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    10141 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10201 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10261 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
    10321 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
    10381 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    10441 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    10501 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    10561 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    10621 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    10681 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    10741 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
    10801 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10861 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10921 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10981 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    11041 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    11101 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    11161 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    11221 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    11281 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    11341 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    11401 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    11461 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    11521 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    11581 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgatt
//
