LOCUS       sequence-215141-       11596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor with point mutation affecting ability to 
            bind actin in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Rothenberg KE, Scott DW, Christoforou N, Hoffman BD
  TITLE     Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable 
            Effective Directed Cell Migration.
  JOURNAL   Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 
            10.1016/j.bpj.2018.02.019.
  PUBMED    29642037
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2018.02"; journalName: "Biophys J"; date: 
            "2018-04-10- 10"; volume: "114"; issue: "7"; pages: "1680-1694"
COMMENT     Sequence Label: pRRL-VinculinTS-I997A
FEATURES             Location/Qualifiers
     source          1..11596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        188..567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        568..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     722..742
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        816..834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3528..4235
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4356..4358,4359..4361,4362..5072)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4401..4422)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4662..4681)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5009..5030
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5646..6234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5699..5719)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6117..6128)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6325..6558
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6630..6751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6667..6686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6721..6740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6791..6926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6947..6965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6975..6992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6975..6991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6984..7006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7133..7588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7220..7239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7430..7451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7614..7718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7719..7787,7788..8579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7937..7956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8750..9338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9239..9258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9492..9509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9626..9647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9662..9667,9668..9685,9686..9692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9700..9716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9705..9727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9724..9740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9724..9740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9759..9779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9761..9779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9807..10033
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10034..10214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10261..10386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10879..11112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11297..11341
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
       61 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
      121 aaaattttat cgatggatcc cccgggctcg agttcgaaga tcgatgtacg ggccagatat
      181 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      241 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      301 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      361 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      421 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      481 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      541 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      601 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      661 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      721 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      781 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      841 caagctggct agcgtttaaa cttaagcttg ccatgcccgt cttccacacg cgcaccatcg
      901 agagcatctt ggagcccgtg gctcagcaga tctcccacct ggtcatcatg cacgaggagg
      961 gggaggtaga cggcaaggcc atcccggacc tcaccgcccc cgtgtcggcc gtgcaggccg
     1021 ctgtcagcaa cctggtgcgg gttggaaaag aaactgtgca gacaacagaa gaccagatct
     1081 tgaaaaggga tatgccacca gcattcatca aagtagagaa tgcctgcacc aagctcgttc
     1141 gagcagccca gatgctgcaa gcagatcctt attcagtacc agctcgtgac tacctaattg
     1201 atggatcaag aggcatcctt tctggaacat cagacttact tctgacattt gatgaagcgg
     1261 aggtccgtaa aatcatccgt gtctgcaaag gaatattgga atatctgact gtggcagaag
     1321 tagtagagac tatggaggat ttggtgacat atacaaagaa tctagggcca ggaatgacaa
     1381 agatggcgaa aatgatcgat gagagacaac aggaattaac tcatcaggaa catagggtta
     1441 tgctggtgaa ctccatgaat actgtgaagg agctattgcc tgtacttatt tcagctatga
     1501 agatctttgt aaccacaaaa aacactaaaa gccagggaat agaagaggcc ttgaaaaatc
     1561 gcaatttcac agtagagaaa atgagtgctg agataaatga aataatccgt gtattacaac
     1621 tcacttcctg ggatgaagat gcctgggcca gcaaggacac tgaagccatg aaaagagctt
     1681 tagccctaat agattcaaag atgaaccagg caaaaggctg gctgagagat ccaaacgcac
     1741 ctccagggga tgctggtgag caagcaataa ggcagatcct tgatgaagct ggaaaagcag
     1801 gagaattgtg tgcaggcaaa gaacgcaggg agattctggg aacatgcaaa actttgggcc
     1861 aaatgactga tcaacttgct gatctccgag ctagaggaca gggtgctaca ccgatggcaa
     1921 tgcagaaggc acagcaggtg tcacaaggcc tggatttgct cactgcaaaa gtggagaatg
     1981 cagcccggaa attggaggcc atgacaaact ctaagcaggc tattgcaaag aagattgatg
     2041 ctgctcagaa ctggcttgcg gatcccaacg gcggaagtga aggagaagaa cacattcgag
     2101 gaattatgtc tgaagcaagg aaagttgcag aattgtgtga ggagcctaaa gaaagagatg
     2161 atatccttcg ctccttgggg gaaatctctg ctctgacagc taagctgtca gatctgcgac
     2221 gacatgggaa aggcgactct cctgaggccc gtgcattggc caagcaaata gctacatcac
     2281 ttcagaactt acagtccaaa acaaacagag ctgtagcaaa tactaggcca gttaaagctg
     2341 ctgtccattt ggagggcaag attgagcaag cccagaggtg gatagataat cctacagttg
     2401 atgatcgggg agtaggccag gcagcaattc ggggtttggt tgcagaaggt cgtcgtctag
     2461 ccaatgtcat gatgggacct tatcgtcagg acctgcttgc caaatgtgac cgtgtagacc
     2521 agctggctgc tcagcttgct gaccttgcag caagagggga aggagagtct cctcaggcta
     2581 gggcaattgc tgctcagctt caggactccc tgaaggatct caaagcacgg atgcaagaag
     2641 caatgaccca ggaggtgtct gatgttttca gtgacactac aacccctatt aagttgttag
     2701 cagtagcagc cactgctcct tctgatactc ccaatagaga agaggtgttt gaagaaagag
     2761 cagcaaattt tgaaaaccat gctgctagac tgggagcaac agcagaaaaa gcagctgcag
     2821 ttggaactgc taataaaact actgtggaag gcattcaggc aacagtcaaa tctgcaaggg
     2881 agcttacacc acaggtagta tcggctgctc gaatcctcct gagaaatcct ggaaatcaag
     2941 ctgcttatga gcattttgag acaatgaaaa accaatggat tgataatgta gaaaagatga
     3001 cagggctggt ggatgaggcc atcgatacca agtctctgtt ggatgcatca gaagaggcta
     3061 ttaagaagga tcttgataaa tgtaaagttg caatggccaa tatgcaacct cagatgctgg
     3121 tagctggagc caccagcatt gctagacgag cgaaccgcat cctgcttgtg gcaaaacggg
     3181 aggttgaaaa ttcagaagac cctaaattca gggaggctgt taaagcagct tctgatgagc
     3241 tgagcaaaac catatcaccg atggtaatgg atgctaaagc tgtagcagga aatatctctg
     3301 atcctggttt gcagaagagt ttcttggatt ctggatacag gattctggga gctgtggcca
     3361 aagtcagaga agcctttcag cctcaggagc cagactttcc ccctcctcct cctgacctag
     3421 agcatctcca tctgactgat gagcttgctc ctccaaaacc accacttcca gaaggtgagg
     3481 ttcccccacc cagaccacca ccccctgaag aaaaagatga ggtcgagatg gtgagcaagg
     3541 gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg
     3601 gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg
     3661 gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca
     3721 ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc
     3781 ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg
     3841 aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct
     3901 acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga
     3961 ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg
     4021 acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca
     4081 tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg
     4141 agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc
     4201 gcaactccac cgacggcatg gacgagctgt acaaggggcc aggtggtgca gggccaggtg
     4261 gtgcagggcc aggtggtgca gggccaggtg gtgcagggcc cggtggtgca ggtccaggtg
     4321 gtgcaggtcc aggtggtgca ggtccaggtg gtgctatggt gagcaagggc gaggagctgt
     4381 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4441 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4501 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4561 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4621 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4681 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4741 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4801 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     4861 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4921 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga
     4981 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5041 ggatcactct cggcatggac gagctgtaca aggcggccgc ggagttccca gagcagaaag
     5101 caggagaagc tattaatcag cccatgatga tggctgctag gcagttgcat gacgaggctc
     5161 ggaaatggtc tagcaagggt aacgacatca ttgctgctgc taaacgaatg gcgctgctca
     5221 tggctgagat gtcacgcctg gtgcgaggag gcagcggaaa caagcgtgcc ctcatccagt
     5281 gtgcaaaaga tattgctaag gcatcggatg aagtcactcg attggccaaa gaggtggcca
     5341 agcaatgtac tgataaacgc attagaacaa acctcttaca ggtctgtgag cgaatcccaa
     5401 ccatcagcac gcagctcaaa gctctttcca cagtgaaagc taccatgctg ggcaggacta
     5461 acatcagcga tgaagaatca gaacaggcaa ctgagatgtt ggttcataac gcccagaacc
     5521 tcatgcagtc tgtgaaggaa actgtgagag aagctgaagc agcatccatt aagataagaa
     5581 cagatgccgg attcactctg cgctgggtca gaaagacccc atggtatcag taatctagag
     5641 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5701 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5761 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5821 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5881 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5941 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6001 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6061 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6121 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6181 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6241 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6601 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6661 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6721 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6841 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6901 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6961 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7021 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7081 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7141 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7201 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7261 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7321 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7381 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7441 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7501 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7561 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7741 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7801 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7861 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7921 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7981 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8041 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8101 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8161 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8221 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8281 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8341 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8401 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8461 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8521 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8581 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8881 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9361 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9481 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9541 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9601 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9661 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9721 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9781 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9841 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9901 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9961 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
    10021 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10201 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    10261 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10321 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10381 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10441 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10501 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10561 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10621 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10681 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10741 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10801 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10861 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10921 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10981 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    11041 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    11101 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    11161 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    11221 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    11281 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    11341 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    11401 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    11461 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    11521 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    11581 gaacggatct cgacgg
//
