LOCUS       sequence-215146-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(del6) C256A
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     365..387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     379..396
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     380..396
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(991..1010)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(992..1010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1292..1309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1463..2051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1543..1562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2222..3013,3014..3082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2845..2864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3083..3187)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3255..3273
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      301 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      361 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgc cccttcatca
      421 ccaacgtagg agtctttttg acccataccg accatgatac cttggtgtct tggtctaccg
      481 acgatagatg ggaagacagc acgaggagcg tcgtcaccgg caaaaccggc tttacacata
      541 ccagaaccgt tatcaataac caaagcagca acctctaaac atataatata gcaacaaaaa
      601 gaatgaagca atcgatgtta ttacatgaga cacagtagca aataaaagaa gaatatcact
      661 atcacttatc acgaaaatat ttcagttttt ttttttttcc aaatttcaag cccctattta
      721 ttccaataat atcgtggtta ttacagatca gtcaatatag gaggttatgg gagagtgaaa
      781 aatagtaaaa aaaggtaaaa gagaaatctc tcgagcaatt gggaccgtgc aattcttctt
      841 acagttaaat gggatggtgc aagcgctaga acataccaga atccattgtt aattcagtaa
      901 attttcgatc ttgggaagaa aaaagcagta agcgtgaaaa atctaaaagg gggatcctct
      961 agagtcgacc tgcagcccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
     1021 gccagctggg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1081 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     1141 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgcatcag
     1201 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     1261 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1861 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3181 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3241 cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
