LOCUS       sequence-215147-        3282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(BrC)
FEATURES             Location/Qualifiers
     source          1..3282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(227..244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(398..986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(478..497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1157..1948,1949..2017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1780..1799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2018..2122)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2190..2208
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2581..2603
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2595..2612
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2596..2612
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3208..3227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3209..3227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
       61 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      121 cacgtagcgc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      181 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
      241 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      301 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
      841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1141 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1201 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1261 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1321 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1381 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1441 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1501 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1561 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1621 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1681 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1741 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     1801 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     1861 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     1921 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     1981 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2041 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2101 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     2161 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     2221 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     2281 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     2341 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     2401 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     2461 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2521 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2581 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgggctgcag gtcgactcta
     2641 gaggatcccc ttcatcacca acgtaggagt ctttttgacc cataccgacc atgatacctt
     2701 ggtgtcttgg tctaccgacg atagatggga agacagcacg aggagcgtcg tcaccggcaa
     2761 aaccggcttt acacatacca gaaccgttat caataaccaa agcagcaacc tctaaacata
     2821 taatatagca acaaaaagaa tgaagcaatc gatggtagta catgagaaca gtagcaaata
     2881 aaagaagaat atcactatca cttatcacga aaatatttca gttttttttt ttttccaaat
     2941 ttcaagcccc tatttattcc aataatatcg tggttattac agatcagtca atataggagg
     3001 ttatgggaga gtgaaaaata gtaaaaaaag gtaaaagaga aatctctcga gcaattggga
     3061 ccgtgcaatt cttcttacag ttaaatggga tggtgcaagc gctagaacat accagaatcc
     3121 attgttaatt cagtaaattt tcgatcttgg gaagaaaaaa gcagtaagcg tgaaaaatct
     3181 aaaaggggcg agctcgaatt ccggtctccc tatagtgagt cgtattaatt tcgataagcc
     3241 agctgggcct cgcgcgtttc ggtgatgacg gtgaaaacct ct
//
