LOCUS       sequence-215149-        3282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(BrG)
FEATURES             Location/Qualifiers
     source          1..3282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     284..306
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     298..315
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     299..315
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(911..930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(912..930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1212..1229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1383..1971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1463..1482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2142..2933,2934..3002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2765..2784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3003..3107)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3175..3193
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
       61 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      121 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
      181 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      241 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      301 aaaacgacgg ccagtgccaa gcttgggctg caggtcgact ctagaggatc cccttcatca
      361 ccaacgtagg agtctttttg acccataccg accatgatac cttggtgtct tggtctaccg
      421 acgatagatg ggaagacagc acgaggagcg tcgtcaccgg caaaaccggc tttacacata
      481 ccagaaccgt tatcaataac caaagcagca acctctaaac atataatata gcaacaaaaa
      541 gaatgaagca atcgatgcta gtacatgaga acagtagcaa ataaaagaag aatatcacta
      601 tcacttatca cgaaaatatt tcagtttttt tttttttcca aatttcaagc ccctatttat
      661 tccaataata tcgtggttat tacagatcag tcaatatagg aggttatggg agagtgaaaa
      721 atagtaaaaa aaggtaaaag agaaatctct cgagcaattg ggaccgtgca attcttctta
      781 cagttaaatg ggatggtgca agcgctagaa cataccagaa tccattgtta attcagtaaa
      841 ttttcgatct tgggaagaaa aaagcagtaa gcgtgaaaaa tctaaaaggg gcgagctcga
      901 attccggtct ccctatagtg agtcgtatta atttcgataa gccagctggg cctcgcgcgt
      961 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     1021 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     1081 tgtcggggcg cagccatgac ccagtcacgt agcgcatcag agcagattgt actgagagtg
     1141 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     1201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1801 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     1861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2281 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3001 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3061 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3121 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3181 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3241 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg at
//
