LOCUS       sequence-215150-        3334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(AcAc)
FEATURES             Location/Qualifiers
     source          1..3334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..82
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     364..383
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        364..382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1031..1048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1031..1047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1040..1062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1435..1453)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1521..1625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1626..1694,1695..2486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1844..1863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2657..3245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3146..3165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
       61 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
      121 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      181 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      241 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      301 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcccagct ggcttatcga
      361 aattaatacg actcactata gggagaccgg aattcgagct cgcccctttt agatttttca
      421 cgcttactgc ttttttcttc ccaagatcga aaatttactg aattaacaat ggattctggt
      481 atgttctagc gcttgcacca tcccatttaa ctgtaagaag aattgcacgg tcccaattgc
      541 tcgagagatt tctcttttac ctttttttac tatttttcac tctcccataa cctcctatat
      601 tgactgatct gtaataacca cgatattatt ggaataaata ggggcttgaa atttggaaaa
      661 aaaaaaaaac tgaaatattt tcgtgataag tgatagtgat attcttcttt tatttgctac
      721 tgtgtctcat gtactaacat cgattgcttc attctttttg ttctatatta tatgtttaca
      781 cgttgctgct ttggttactt cattcttttt gttgctatat tatatgttta cacgttgctg
      841 ctttggttat tgataacggt tctggtatgt gtaaagccgg ttttgccggt gacgacgctc
      901 ctcgtgctgt cttcccatct atcgtcggta gaccaagaca ccaaggtatc atggtcggta
      961 tgggtcaaaa agactcctac gttggtgatg aaggggatcc tctagagtcg acctgcagcc
     1021 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1081 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1141 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     1201 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     1261 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     1321 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     1381 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     1441 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1501 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1561 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1621 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1681 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1741 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1801 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1861 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1921 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1981 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2041 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2101 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2161 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2221 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2281 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2341 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2401 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2461 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2521 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3301 acatgttctt tcctgcgtta tcccctgatt ctgt
//
