LOCUS       sequence-215152-        3284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(del6) A259C
FEATURES             Location/Qualifiers
     source          1..3284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(509..526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(509..525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(518..540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(913..931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        999..1103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1104..1172,1173..1964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1322..1341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2135..2723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2624..2643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2877..2894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3176..3195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3176..3194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attctggtat gttctagcgc ttgcaccatc ccatttaact gtaagaagaa ttgcacggtc
       61 ccaattgctc gagagatttc tcttttacct ttttttacta tttttcactc tcccataacc
      121 tcctatattg actgatctgt aataaccacg atattattgg aataaatagg ggcttgaaat
      181 ttggaaaaaa aaaaaaactg aaatattttc gtgataagtg atagtgatat tcttctttta
      241 tttgctactg tgtctcatgt actaccatcg attgcttcat tctttttgtt gctatattat
      301 atgtttagag gttgctgctt tggttattga taacggttct ggtatgtgta aagccggttt
      361 tgccggtgac gacgctcctc gtgctgtctt cccatctatc gtcggtagac caagacacca
      421 aggtatcatg gtcggtatgg gtcaaaaaga ctcctacgtt ggtgatgaag ggggatcctc
      481 tagagtcgac ctgcagccca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
      541 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      601 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      661 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      721 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
      781 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
      841 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      901 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      961 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1081 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1141 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1201 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1261 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1321 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1381 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1441 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1501 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1561 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1621 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2881 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     2941 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gcgctacgtg
     3001 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3061 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3121 agaggttttc accgtcatca ccgaaacgcg cgaggcccag ctggcttatc gaaattaata
     3181 cgactcacta tagggagacc ggaattcgag ctcgcccctt ttagattttt cacgcttact
     3241 gcttttttct tcccaagatc gaaaatttac tgaattaaca atgg
//
