LOCUS       sequence-215159-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  brr2-R1107M (endogenous promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Brr2 is a splicing fidelity factor
  JOURNAL   bioRxiv 2018. 354514
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2018. 354514"
COMMENT     Sequence Label: brr2-R1107M pRS313
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..85
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     319..336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    453..474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(489..494,495..512,513..519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    527..543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     532..554
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     551..567
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     551..567
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7705..7722)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7705..7721)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7714..7736)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8236..8528
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8529..8982
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(9672..10334)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(10335..10522)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        10944..11048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11049..11117,11118..11909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11267..11286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12080..12503,1..165)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
       61 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      241 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      301 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      361 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      421 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      481 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      541 caatttcaca caggaaacag ctatgacatg attacgaatt cgagctccat tttgttattt
      601 gcgagcccaa tgaactgaga ctatattgta ataagggccg tttgttcaaa ttcgtactat
      661 ccatatcatt cagacaggaa caaacttgat aattagtggt gttgaatcaa gtactattaa
      721 cgcattgtga tgatctctca gatatcaatg cgtgtttgct tctttgacga tctaatattg
      781 aatattgtcg ctctttacga gaatattaaa aatttatgct actaaagcta tgtctactat
      841 aaaggaataa tacactaata gagataatct tgagtaaaat aaggtgaagg taaatataag
      901 taattgcttt ggaagattta ccgtgagcct tcgttattaa aaatgactga gcatgaaacg
      961 aaggataagg ccaaaaaaat tagagaaatt tatcgttatg atgagatgtc caataaggtg
     1021 ttgaaagttg ataaacgctt tatgaatacc agccaaaatc ctcaaaggga tgcggaaata
     1081 tcacaaccaa aatccatgag tggtagaatt agtgctaagg atatgggaca gggcctgtgc
     1141 aataacatta ataaaggact aaaagaaaat gatgtagccg ttgaaaaaac tggaaagagc
     1201 gcatcattga aaaaaattca gcagcataac accattttga attcttcctc cgatttcagg
     1261 ttacattatt atccaaagga tccctcaaat gtcgaaactt acgagcagat tttgcagtgg
     1321 gttacagaag ttttaggcaa tgatataccc catgatctta ttattggaac tgctgatata
     1381 ttcatcagac aattgaaaga aaatgaggaa aatgaggatg gtaatattga agagcgaaag
     1441 gaaaagatcc agcatgagct aggcatcaat atagactccc tgaagtttaa cgaattagtt
     1501 aaacttatga aaaacattac cgattatgaa acacatcctg acaatagcaa taaacaagca
     1561 gtagcaattc ttgccgatga tgaaaaatca gatgaagaag aagtaacaga aatgtccaac
     1621 aatgctaatg ttcttggggg agaaatcaat gataatgaag atgatgatga ggaatatgat
     1681 tacaatgacg ttgaagtcaa ttctaaaaaa aaaaacaaga gggcattgcc aaacattgaa
     1741 aacgatataa ttaaactctc cgatagtaaa acaagtaata tcgagagcgt tccgatatac
     1801 tcaatcgatg aatttttttt gcagagaaaa cttcggtcag agcttggtta caaggatacc
     1861 tctgtaatac aagacctgtc agaaaaaatt ttgaatgata ttgaaacact tgagcataat
     1921 ccagtagcgt tggagcagaa gttggttgat ctgcttaaat tcgagaatat atcactggct
     1981 gaatttattc tcaagaatag atctacaatt ttttggggca tacgactggc gaagagcaca
     2041 gagaatgaaa taccgaactt aatagagaaa atggttgcaa aaggtttgaa tgatcttgtg
     2101 gaacagtata aatttagaga aactacacac tcaaagcgtg aactcgattc cggtgacgac
     2161 cagccacagt cttcggaagc aaaaagaaca aaattcagta atccagcaat accgcccgtc
     2221 atagatttgg aaaagattaa attcgacgaa agttctaaat taatgaccgt gacaaaagta
     2281 tccctaccgg aaggatcatt caaaagggtg aaaccacaat atgacgaaat acatattcca
     2341 gctcctagca aacctgttat tgattatgag ttgaaagaaa ttacttctct accagattgg
     2401 tgtcaggagg cgtttccttc atcggaaaca acgtccttaa accctataca atcaaaggtt
     2461 tttcacgcag catttgaagg tgattcaaac atgctaatat gtgcacccac tggttcaggt
     2521 aagacgaata tcgcgttatt gacggtattg aaagccttat cccatcatta taacccgaaa
     2581 actaagaaat taaacctttc tgcatttaaa attgtttata tagcaccctt gaaagcctta
     2641 gtacaggaac aagtccgaga atttcaaagg aggttggctt ttctaggtat aaaagtcgct
     2701 gaattgactg gtgattctag gcttagtaga aagcaaattg acgagacgca ggttttggtg
     2761 tctacgccag agaaatggga cataactact agaaattcaa ataatttggc catcgtagaa
     2821 cttgttcgtt tgttgattat agatgaaata catttgttac atgatgacag gggaccagta
     2881 ttggaaagca tcgtggctag gacattttgg gcttccaaat atggccagga ataccccaga
     2941 ataataggcc tttctgccac cttaccaaat tatgaggatg tcggtaggtt tttaagagtt
     3001 ccgaaggaag gattgtttta ttttgactcc tcatttagac cgtgtcctct atctcaacag
     3061 ttttgtggca ttaaggaacg gaactccttg aaaaagctga aggctatgaa tgatgcttgt
     3121 tatgaaaagg tattggaatc catcaatgaa ggtaatcaaa taatcgtgtt tgttcattct
     3181 cgaaaagaaa catcacgaac agcaacatgg ttgaaaaata aatttgctga agagaacatt
     3241 actcataaat taaccaaaaa tgatgcagga tcgaagcaga ttttaaagac agaagcagcc
     3301 aacgtactcg atccaagtct acgaaagttg attgaaagcg gtatagggac gcatcacgca
     3361 gggctgacca gaagtgacag atccttatct gaagatttat ttgcagacgg gttgttgcaa
     3421 gtattggtat gcactgcgac gttagcatgg ggtgttaatc tacctgctca tacagtgatt
     3481 ataaaaggaa cagatgtcta ttcacctgaa aaggggtcct gggaacaact ttctccacaa
     3541 gatgtacttc aaatgttagg tagagcaggc agaccgagat atgacacatt tggcgagggt
     3601 attataataa cagaccaatc gaatgttcaa tattatctct cggttttaaa tcaacaatta
     3661 ccaatagaat cgcaatttgt ttcaaaatta gttgacaact taaatgcaga agttgtcgcg
     3721 ggtaacatta agtgcaggaa tgatgctgtg aattggttgg cgtatactta cctatatgtc
     3781 agaatgttag cttcccctat gctttacaaa gtacctgata tttcaagtga tgggcaattg
     3841 aaaaagttta gagaaagctt agtccattcg gccctatgca ttttaaaaga acaagagttg
     3901 gtgttatatg atgctgaaaa tgatgtaatt gaggcgaccg atctaggaaa tatagcgtcg
     3961 tcattttaca taaatcatgc ttccatggat gtttacaata gagagttaga tgaacataca
     4021 actcagattg atctttttag aatattctca atgtcggaag agtttaagta cgtatcggtg
     4081 agatatgaag agaaaagaga actaaaacaa cttctagaga aagcacccat tcctatcaga
     4141 gaagatattg atgatccttt agcaaaagtg aatgtactac tgcagtctta tttttcccag
     4201 ctaaagttcg aaggttttgc acttaattct gatatagtgt ttattcacca aaatgctggt
     4261 atgttattgc gtgctatgtt tgaaatatgc ttgaagagag gatggggaca tcccacacgg
     4321 atgttgttaa atctttgcaa atctgccaca actaaaatgt ggccaacaaa ctgtccttta
     4381 agacagttta aaacatgccc tgtcgaagtg attaaaaggc tggaagcctc tacagtaccg
     4441 tggggagatt atctacagct agaaacacct gccgaagttg gtcgtgcaat cagatctgaa
     4501 aaatatggaa aacaggttta cgatttattg aaaagatttc ccaaaatgtc cgtaacatgc
     4561 aacgctcagc caattacccg tagcgttatg aggttcaaca ttgaaataat agccgactgg
     4621 atatgggata tgaatgtaca cggttccctt gaaccttttt tgctcatgct agaagacact
     4681 gatggtgatt caatacttta ttatgacgta ctattcatca ctccagatat agttggtcat
     4741 gaatttacct tatcctttac ttatgagtta aagcagcaca atcaaaataa cttaccacct
     4801 aatttcttcc ttactttgat atcagaaaat tggtggcaca gtgaattcga gattccagtt
     4861 tccttcaatg gatttaaact tccgaaaaaa tttcctcctc ctactcccct tctggagaat
     4921 attagtattt caacatcaga gcttggcaat gatgattttt ccgaagtatt tgaatttaaa
     4981 actttcaaca agattcagag ccaggtcttc gagtctctct acaattcgaa tgactcggtc
     5041 tttgtaggtt caggaaaggg cacaggtaaa acagctatgg ctgaattagc actgttaaat
     5101 cattggagac agaataaagg aagagcggtt tatatcaatc cgtctgggga aaaaattgat
     5161 tttttgctat ctgattggaa taaaaggttc tctcatcttg ctggtggtaa gataataaat
     5221 aagctaggaa atgatccttc actaaattta aaattattag caaaaagcca tgttctcttg
     5281 gctacccccg tgcaatttga gcttttgtca cgtcgttgga gacaaaggaa gaacatccaa
     5341 agtttagaat taatgatata tgatgacgct catgagatta gccaaggtgt ttacggtgct
     5401 gtgtatgaaa cgctaatttc tagaatgata tttatagcaa cccaacttga aaagaaaatt
     5461 cgatttgttt gcctttcgaa ctgtttggca aatgctcgtg attttggtga atgggctggt
     5521 atgacgaagt caaacatcta caacttttct ccaagcgaaa gaattgagcc tcttgaaatt
     5581 aatattcagt catttaaaga tgttgagcac atatcattca atttttcgat gcttcaaatg
     5641 gcatttgaag catctgctgc tgcagcggga aatcgtaatt ccagttcggt gttcttacca
     5701 tctagaaagg attgtatgga agttgcgtct gcatttatga agttttcaaa agcaattgaa
     5761 tgggatatgt tgaacgttga agaagagcaa attgtaccat acattgaaaa gttaacagat
     5821 ggtcatctaa gagcaccttt aaaacacggt gttggtattt tgtataaggg gatggcatca
     5881 aatgatgaaa gaattgtgaa aaggttatac gaatatggtg cggtatcggt tttactcatt
     5941 tctaaggatt gttctgcatt cgcttgtaaa actgatgaag tgattatttt aggaaccaac
     6001 ctttatgatg gtgcagagca taaatatatg ccatatacca taaatgaact tctagaaatg
     6061 gtgggactag ctagtggtaa cgattcaatg gcaggcaagg ttttaatttt gacaagtcac
     6121 aacatgaagg cctactacaa aaaattctta attgagcctt tgcctactga gagttaccta
     6181 cagtatatta ttcatgatac tctgaacaac gagatagcaa attctattat acaaagcaaa
     6241 caagattgtg ttgactggtt tacatattcg tatttctatc gtcgtatcca tgtgaatcca
     6301 agctattatg gcgtaaggga tacgtctcca catggaattt cagttttttt gagtaattta
     6361 gttgaaactt gtttgaatga cttagttgaa tcatccttca ttgaaattga cgatacagaa
     6421 gctgaagtaa ccgcagaagt taatggagga gatgatgaag caactgaaat catttctacc
     6481 ttgagcaacg ggctaattgc ttctcactat ggcgtatcat tttttactat tcagtcattc
     6541 gtttcttctt tatcgaatac gtcaacgctc aaaaacatgc tgtatgttct ttcaacagca
     6601 gtagaattcg aaagtgttcc actcagaaaa ggagacagag cattacttgt caaattaagt
     6661 aaaagactac cattgagatt ccctgaacac acctcttctg gttcggtgag ctttaaagtt
     6721 ttcttattgt tacaagcata tttttcacgt cttgaattac ctgtcgactt tcaaaatgat
     6781 ttaaaagaca ttctagaaaa ggttgttcca ctaattaacg tagtggttga tatcctttca
     6841 gcaaacgggt atttgaatgc tactaccgcc atggatttgg cgcagatgtt gattcagggt
     6901 gtatgggacg ttgataatcc gttgagacag attccacact tcaacaacaa aattttggaa
     6961 aaatgtaaag aaattaacgt cgagacggtc tatgatataa tggcccttga ggatgaagag
     7021 agggatgaaa ttttaacgct tactgattca cagcttgcgc aggtcgccgc gtttgttaac
     7081 aattacccca acgttgaact tacttattcg ttgaataatt ctgattcgtt aatttcaggt
     7141 gtaaaacaga aaattaccat tcaattgaca agagatgttg aacctgagaa cttacaagta
     7201 acatctgaaa agtatccgtt tgataagcta gaaagttggt ggttggtttt aggtgaagta
     7261 tctaaaaaag agctttatgc gatcaagaaa gttactttaa ataaagagac tcagcaatat
     7321 gaattggaat ttgacactcc gacatctggt aaacataact taacaatttg gtgtgtctgt
     7381 gattcatatc ttgacgcaga taaagagttg tcctttgaaa taaatgtgaa ataaaatcat
     7441 tgtttagtcc tggataatcg aatggatttc aatatataac attctcttgc ggctatttaa
     7501 tctaattgtg atatatacag tttatagcaa aagcgtatca ttgcatcact gcatttctat
     7561 atcttcatct tcatcttcat cctgttctcc ttctttgttt tcgttttcaa taaatccacc
     7621 ttgatcctct agagtcgacc tgcaggggtg gagctcggta cccggggatc ctctagagtc
     7681 gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7741 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7801 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7861 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataactag
     7921 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc
     7981 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa
     8041 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa
     8101 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cacattagca tacatatatt
     8161 acacgagcca gaaatagtaa cttttgccta aatcacaaat tgcaaaattt aattgcttgc
     8221 aaaaggtcac atgcttataa tcaacttttt taaaaattta aaatactttt ttatttttta
     8281 tttttaaaca taaatgaaat aatttattta ttgtttatga ttaccgaaac ataaaacctg
     8341 ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa
     8401 tgccgaggct ttcatagctt aaactcttta cagaaaatag gcattataga tcagttcgag
     8461 ttttcttatt cttccttccg gttttatcgt cacagtttta cagtaaataa gtatcacctc
     8521 ttagagttag cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg
     8581 cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg
     8641 taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg
     8701 agtagtattt atttaagtat tgtttgtgca cttgcctgca ggccttttga aaagcaagca
     8761 taaaagatct aaacataaaa tctgtaaaat aacaagatgt aaagataatg ctaaatcatt
     8821 tggctttttg attgattgta caggaaaata tacatcgcag ggggttgact tttaccattt
     8881 caccgcaatg gaatcaaact tgttgaagag aatgttcaca ggcgcatacg ctacaatgac
     8941 ccgattcttg ctagcctttt ctcggtcttg caaacaaccg ccggcagctt agtatataaa
     9001 tacacatgta catacctctc tccgtatcct cgtaatcatt ttcttgtatt tatcgtcttt
     9061 tcgctgtaaa aactttatca cacttatctc aaatacactt attaaccgct tttactatta
     9121 tcttctacgc tgacagtaat atcaaacagt gacacatatt aaacacagtg gtttctttgc
     9181 ataaacacca tcagcctcaa gtcgtcaagt aaagatttcg tgttcatgca gatagataac
     9241 aatctatatg ttgataatta gcgttgcctc atcaatgcga gatccgttta accggacctt
     9301 ttctttgact acaacacaaa aatcaagcac agaacgtcat acgaatccgc aactacattc
     9361 tgggcattat gggctggact tgccacgaag gagcaagcac agaaaatggt ggagaaagca
     9421 ctacccaagt tagagatgct tggaggttta gctgcatgta cggagcgttc tcgagttcaa
     9481 gagaaaaaaa aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac
     9541 gtatagaatg atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac
     9601 tgacattcat aggtatacat atatacacat gtatatatat cgtatgctgc agctttaaat
     9661 aatcggtgtc actacataag aacacctttg gtggagggaa catcgttggt accattgggc
     9721 gaggtggctt ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga
     9781 tcattcttgc ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaagctt
     9841 tcaagaaaat gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca
     9901 agttcgacaa ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca
     9961 tccaaaggcg caaatcctga tccaaacctt tttactccac gcacggcccc tagggcctct
    10021 ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt
    10081 aagtcaccaa tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc
    10141 atatggtcca gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt
    10201 tctgctactg cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca
    10261 ccctttaaag agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct
    10321 ttctgctctg tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc
    10381 gaagaaatca cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa
    10441 gaaaaaaaaa gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat
    10501 aaaaaaatat agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa
    10561 aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc
    10621 ctagcgctca ccaagctctt aaaacgagaa ttaagaaaaa gtcgtcatct ttatactaag
    10681 ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
    10741 aaaataaaat cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata
    10801 ctgtgatgcc atggtgtccg acttatgccc gagaagatgt tgagcaaact tatcgcttat
    10861 ctgcttctca tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gcggatccag
    10921 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10981 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11041 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11101 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11161 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11221 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11281 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11341 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11401 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11461 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11521 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11581 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11641 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11701 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11761 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11821 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11881 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11941 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12001 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12061 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12481 gcgccacgct tcccgaaggg aga
//
