LOCUS       sequence-215160-       12946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for FLAG-tagged human T1R2 with signal 
            peptide of influenza hemagglutinin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E
  TITLE     Structural architecture of a dimeric class C GPCR based on 
            co-trafficking of sweet taste receptor subunits.
  JOURNAL   J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 
            10.1074/jbc.RA118.006173.
  PUBMED    30723160
REFERENCE   2  (bases 1 to 12946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173."
COMMENT     Sequence Label: pCEP4-HA-Flag-natT1R2
FEATURES             Location/Qualifiers
     source          1..12946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    20..154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(44..63)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     98..117
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(296..318)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      904..2693
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2999..4924)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5359..5377)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5445..5549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5550..5618,5619..6410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5768..5787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6581..7169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7070..7089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7786..7931
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7971..8996
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7971..8996
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(9017..9036)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9061..9109
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9762..10141
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10142..10345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10296..10316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10342..10366
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac
       61 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      121 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      181 gtttcaggtt cagggggagg tggggaggtt ttttaaagca agtaaaacct ctacaaatgt
      241 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaccggtc
      421 gactctagag ccccgccgcc ggacgaacta aacctgacta cggcatctct gccccttctt
      481 cgctggtacg aggagcgctt ttgttttgta ttcggggcag tgcatgtaat cccttcagtt
      541 ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca
      601 caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca
      661 ctgagtggct ttcatcctgg agcagacttt gcatgctgtg gactgcaaca caacattgcc
      721 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag
      781 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac
      841 cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc
      901 ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt
      961 caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt
     1021 cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc
     1081 aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga
     1141 gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat
     1201 agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc
     1261 aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac
     1321 accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc
     1381 tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc
     1441 atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg
     1501 ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt
     1561 tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac
     1621 acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc
     1681 actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg
     1741 gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg
     1801 cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta
     1861 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact
     1921 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca
     1981 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct
     2041 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     2101 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     2161 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     2221 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct
     2281 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc
     2341 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc
     2401 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     2461 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     2521 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     2581 tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca
     2641 tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgcatgctg
     2701 gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt
     2761 gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg
     2821 agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac
     2881 aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt
     2941 tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc
     3001 actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat
     3061 caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat
     3121 cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa
     3181 ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga
     3241 aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc
     3301 caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc
     3361 gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag
     3421 ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg
     3481 caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc
     3541 cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg
     3601 ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc
     3661 caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc
     3721 tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc
     3781 tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc
     3841 tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac
     3901 tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct cctgctcctg
     3961 cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc
     4021 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     4081 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc
     4141 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     4201 ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     4261 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctgctcctg
     4321 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4381 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
     4441 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     4501 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc
     4561 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc
     4621 ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc
     4681 agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt cttggccctg
     4741 atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc
     4801 ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag ccttctggtc
     4861 cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc ccctcgtcag
     4921 acatgattca cactaaaaga gatcaataga catctttatt agacgacgct cagtgaatac
     4981 agggagtgca gactcctgcc ccctccaaca gcccccccac cctcatcccc ttcatggtcg
     5041 ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg aaccatcctc gtcctcatca
     5101 ccaattactc gcagcccgga aaactcccgc tgaacatcct caagatttgc gtcctgagcc
     5161 tcaagccagg cctcaaattc ctcgtccccc tttttgctgg acggtaggga tggggattct
     5221 cgggacccct cctcttcctc ttcaaggtca ccagacagag atgctactgg ggcaacggaa
     5281 gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg ataagctgtc aaacatgaga
     5341 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5401 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5461 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5521 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5581 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5641 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5701 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5761 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     5821 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5881 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5941 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6001 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6061 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     6121 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6181 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6241 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6301 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6361 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6541 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6601 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6661 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6721 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6781 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6841 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6901 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6961 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7021 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7081 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7141 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7201 tggccttttg ctgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg
     7261 ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
     7321 agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc
     7381 catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat
     7441 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
     7501 gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     7561 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     7621 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     7681 cccagcgcgt cggccgccat gccctgcttc atccccgtgg cccgttgctc gcgtttgctg
     7741 gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg
     7801 cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccc
     7861 aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc
     7921 gacccgctta acagcgtcaa cagcgtgccg cagatcccgg gcaatgagat atgaaaaagc
     7981 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     8041 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     8101 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     8161 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     8221 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     8281 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     8341 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     8401 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     8461 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     8521 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     8581 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     8641 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     8701 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     8761 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     8821 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     8881 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     8941 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagggga
     9001 gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     9061 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     9121 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     9181 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     9241 gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg
     9301 ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg
     9361 gtctggtcca cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg
     9421 accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc
     9481 cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg
     9541 acagcttatc atcgcagatc cgggcaacgt tgttgcattg ctgcaggcgc agaactggta
     9601 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
     9661 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     9721 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
     9781 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     9841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     9901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     9961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    10021 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    10081 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    10141 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
    10201 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
    10261 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
    10321 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt
    10381 accagctgct agcgccacca tgaagaccat catcgccctg agctacatct tctgcctggt
    10441 gttcgccgac tacaaggatg acgacgacgg cagccccgga ggagctgctg agaactcgga
    10501 cttctacctg cctggggatt acctcctggg tggcctcttc tccctccatg ccaacatgaa
    10561 gggcattgtt caccttaact tcctgcaggt gcccatgtgc aaggagtatg aagtgaaggt
    10621 gataggctac aacctcatgc aggccatgcg ctttgcggtg gaggagatca acaatgacag
    10681 cagcctgctg cctggtgtgc tgctgggcta tgagatcgtg gatgtgtgct acatctccaa
    10741 caatgtccag ccggtgctct acttcctggc acacgaggac aacctccttc ccatccaaga
    10801 ggactacagt aactacattt cccgtgtggt ggctgtcatt ggccctgaca actccgagtc
    10861 tgtcatgact gtggccaact tcctctccct atttctcctt ccacagatca cctacagcgc
    10921 catcagcgat gagctgcgag acaaggtgcg cttcccggct ttgctgcgta ccacacccag
    10981 cgccgaccac cacatcgagg ccatggtgca gcttatgctg cacttccgct ggaactggat
    11041 cattgtgctg gtgagcagcg acacctatgg ccgcgacaat ggccagctgc ttggcgagcg
    11101 cgtggcccgg cgcgacatct gcatcgcctt ccaggagacg ctgcccacac tgcagcccaa
    11161 ccagaacatg acgtcagagg agcgccagcg cctggtgacc attgtggaca agctgcagca
    11221 gagcacagcg cgcgtcgtgg tcgtgttctc gcccgacctg accctgtacc acttcttcaa
    11281 tgaggtgctg cgccagaact tcactggcgc cgtgtggatc gcctccgagt cctgggccat
    11341 cgacccggtc ctgcacaacc tcacggagct gcgccacttg ggcaccttcc tgggcatcac
    11401 catccagagc gtgcccatcc cgggcttcag tgagttccgc gagtggggcc cacaggctgg
    11461 gccgccaccc ctcagcagga ccagccagag ctatacctgc aaccaggagt gcgacaactg
    11521 cctgaacgcc accttgtcct tcaacaccat tctcaggctc tctggggagc gtgtcgtcta
    11581 cagcgtgtac tctgcggtct atgctgtggc ccatgccctg cacagcctcc tcggctgtga
    11641 caaaagcacc tgcaccaaga gggtggtcta cccctggcag ctgcttgagg agatctggaa
    11701 ggtcaacttc actctcctgg accaccaaat cttcttcgac ccgcaagggg acgtggctct
    11761 gcacttggag attgtccagt ggcaatggga ccggagccag aatcccttcc agagcgtcgc
    11821 ctcctactac cccctgcagc gacagctgaa gaacatccaa gacatctcct ggcacaccat
    11881 caacaacacg atccctatgt ccatgtgttc caagaggtgc cagtcagggc aaaagaagaa
    11941 gcctgtgggc atccacgtct gctgcttcga gtgcatcgac tgccttcccg gcaccttcct
    12001 caaccacact gaagatgaat atgaatgcca ggcctgcccg aataacgagt ggtcctacca
    12061 gagtgagacc tcctgcttca agcggcagct ggtcttcctg gaatggcatg aggcacccac
    12121 catcgctgtg gccctgctgg ccgccctggg cttcctcagc accctggcca tcctggtgat
    12181 attctggagg cacttccaga cacccatagt tcgctcggct gggggcccca tgtgcttcct
    12241 gatgctgaca ctgctgctgg tggcatacat ggtggtcccg gtgtacgtgg ggccgcccaa
    12301 ggtctccacc tgcctctgcc gccaggccct ctttcccctc tgcttcacaa tctgcatctc
    12361 ctgtatcgcc gtgcgttctt tccagatcgt ctgcgccttc aagatggcca gccgcttccc
    12421 acgcgcctac agctactggg tccgctacca ggggccctac gtctctatgg catttatcac
    12481 ggtactcaaa atggtcattg tggtaattgg catgctggcc acgggcctca gtcccaccac
    12541 ccgtactgac cccgatgacc ccaagatcac aattgtctcc tgtaacccca actaccgcaa
    12601 cagcctgctg ttcaacacca gcctggacct gctgctctca gtggtgggtt tcagcttcgc
    12661 ctacatgggc aaagagctgc ccaccaacta caacgaggcc aagttcatca ccctcagcat
    12721 gaccttctat ttcacctcat ccgtctccct ctgcaccttc atgtctgcct acagcggggt
    12781 gctggtcacc atcgtggacc tcttggtcac tgtgctcaac ctcctggcca tcagcctggg
    12841 ctacttcggc cccaagtgct acatgatcct cttctacccg gagcgcaaca cgcccgccta
    12901 cttcaacagc atgatccagg gctacaccat gaggagggac tagtga
//
