LOCUS       sequence-215172-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses IncB tagged with 7 copies of GFP11 under a tetracycline 
            inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Wang X, Hybiske K, Stephens RS
  TITLE     Direct visualization of the expression and localization of 
            chlamydial effector proteins within infected host cells.
  JOURNAL   Pathog Dis. 2018 Mar 1;76(2). pii: 4830102. doi: 
            10.1093/femspd/fty011.
  PUBMED    29390129
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pathog 
            Dis."; date: "2018-03-1"; volume: "76(2). pii: 4830102. doi"; pages:
            " 10.1093/femspd/fty011"
COMMENT     Sequence Label: pTL2-IncB-GFP11x7
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             361..786
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     CDS             790..813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggttcatt ctgtatacaa ttcattggct ccagaaggtt ttagccaagt ctctattcaa
       61 cccagtcaga ttccaaccag caaaaaagta atgattgcga taatgactct ttttgcactc
      121 acagccattg cagcaatagt cctttccatc gttacagttt gtggagggtt tccttttctt
      181 cttgctgcac ttaacaccgt aactattggt gcatgcgtat ccttgccggt attcacttgc
      241 atagctacaa cgttattact tctttgtctc cgtaatatcg aactcctagc cagaccgcaa
      301 gtatttaccc tctccactca attcagccca acaaaacctc aagttgtcga cggttcggga
      361 cgtgaccaca tggtccttca tgagtatgta aatgctgctg ggattacagg tggctctgga
      421 ggtagagatc atatggttct ccacgaatac gttaacgccg caggcatcac tggcggtagt
      481 ggaggacgcg accatatggt actacatgaa tatgtcaatg cagccggaat aaccggaggg
      541 tccggaggcc gggatcacat ggtgctgcat gagtatgtga acgcggcggg tataactggt
      601 gggtcgggcg gacgagacca tatggtgctt cacgaatacg taaacgcagc tggcattact
      661 ggcggatcag gtggcaggga tcacatggta ctccatgagt acgtgaacgc tgctggaatc
      721 acaggcggta gcggcggtcg ggaccatatg gtcctgcacg aatatgtcaa tgctgccggt
      781 atcaccatgg actacaagga tgacgacgat aaggctagct aa
//
