LOCUS       sequence-215203-        9987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GFP under the CAMK2A promoter in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Nehme R, Zuccaro E, Ghosh SD, Li C, Sherwood JL, Pietilainen O, 
            Barrett LE, Limone F, Worringer KA, Kommineni S, Zang Y, 
            Cacchiarelli D, Meissner A, Adolfsson R, Haggarty S, Madison J, 
            Muller M, Arlotta P, Fu Z, Feng G, Eggan K
  TITLE     Combining NGN2 Programming with Developmental Patterning Generates 
            Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission.
  JOURNAL   Cell Rep. 2018 May 22;23(8):2509-2523. doi: 
            10.1016/j.celrep.2018.04.066.
  PUBMED    29791859
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.04"; journalName: "Cell Rep"; date: 
            "2018-05-22- 22"; volume: "23"; issue: "8"; pages: "2509-2523"
COMMENT     Sequence Label: DRH-307
FEATURES             Location/Qualifiers
     source          1..9987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2365..3653
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3676..3685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3682..3684,3685..3687,3688..4401)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3682..3684,3685..3687,3688..4398)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3727..3748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3988..4007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4335..4356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4442..5030
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4495..4515)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4913..4924)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5033..5049)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5034..5050)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5555..5735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5761..5778)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5767..5991
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6037..6465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6124..6143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6334..6355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6474..6494)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6479..6808
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6659..6794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6721..6740
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6856..6903
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6922..7296
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7426..7547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7463..7482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7517..7536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7596..7612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7596..7612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7609..7631)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7620..7636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7644..7650,7651..7668,7669..7674))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7689..7710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7827..7844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7998..8586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8078..8097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8757..9548,9549..9617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9380..9399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9618..9722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9797..9816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacattat ggccttaggt cacttcatct ccatggggtt
     2401 cttcttctga ttttctagaa aatgagatgg gggtgcagag agcttcctca gtgacctgcc
     2461 cagggtcaca tcagaaatgt cagagctaga acttgaactc agattactaa tcttaaattc
     2521 catgccttgg gggcatgcaa gtacgatata cagaaggagt gaactcatta gggcagatga
     2581 ccaatgagtt taggaaagaa gagtccaggg cagggtacat ctacaccacc cgcccagccc
     2641 tgggtgagtc cagccacgtt cacctcatta tagttgcctc tctccagtcc taccttgacg
     2701 ggaagcacaa gcagaaactg ggacaggagc cccaggagac caaatcttca tggtccctct
     2761 gggaggatgg gtggggagag ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg
     2821 tgacagatag gggtgagaaa gcagacagag tcattccgtc agcattctgg gtctgtttgg
     2881 tacttcttct cacgctaagg tggcggtgtg atatgcacaa tggctaaaaa gcagggagag
     2941 ctggaaagaa acaaggacag agacagaggc caagtcaacc agaccaattc ccagaggaag
     3001 caaagaaacc attacagaga ctacaagggg gaagggaagg agagatgaat tagcttcccc
     3061 tgtaaacctt agaacccagc tgttgccagg gcaacggggc aatacctgtc tcttcagagg
     3121 agatgaagtt gccagggtaa ctacatcctg tctttctcaa ggaccatccc agaatgtggc
     3181 acccactagc cgttaccata gcaactgcct ctttgcccca cttaatccca tcccgtctgt
     3241 taaaagggcc ctatagttgg aggtggggga ggtaggaaga gcgatgatca cttgtggact
     3301 aagtttgttc gcatcccctt ctccaacccc ctcagtacat caccctgggg gaacagggtc
     3361 cacttgctcc tgggcccaca cagtcctgca gtattgtgta tataaggcca gggcaaagag
     3421 gagcaggttt taaagtgaaa ggcaggcagg tgttggggag gcagttaccg gggcaacggg
     3481 aacagggcgt ttcggaggtg gttgccatgg ggacctggat gctgacgaag gctcgcgagg
     3541 ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct cgtcagtcaa gccggttctc
     3601 cgtttgcact caggagcacg ggcaggcgag tggcccctag ttctgggggc agcgggggat
     3661 ccccgggtac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4081 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4141 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4201 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4261 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4321 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4381 atggacgagc tgtacaagta aagcggccgc gactctagaa ttcgatatca agcttatcga
     4441 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4501 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4561 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4621 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4681 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4741 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4801 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4861 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4921 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4981 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     5041 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5101 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5161 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5221 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5281 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5341 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5401 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5461 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5521 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5581 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5641 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5701 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     5761 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5821 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5881 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5941 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6001 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6061 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6121 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6181 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6241 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6301 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6361 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6421 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6481 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6541 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6601 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6721 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6781 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6841 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6901 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6961 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7021 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7081 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7141 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7201 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7261 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7381 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7561 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9721 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     9781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9841 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9901 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9961 gcgatgtacg ggccagatat acgcgtt
//
