LOCUS       sequence-215241-       11460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Xu W, Lipscombe D
  TITLE     Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively 
            hyperpolarized membrane potentials and are incompletely inhibited by
            dihydropyridines.
  JOURNAL   J Neurosci. 2001 Aug 15. 21(16):5944-51.
  PUBMED    11487617
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2001 Aug 15. 21(16):5944-51."
COMMENT     Sequence Label: CaV1.3e[8a,11,31b,Delta-32,Delta-42a] mut
FEATURES             Location/Qualifiers
     source          1..11460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7399..7440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7450..7467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7490..7507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7496..7720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7766..8194
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7853..7872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8063..8084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8203..8223)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8247..8457
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8269..8465
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8316..8451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8378..8397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8513..8560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8579..8977
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9135..9256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9172..9191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9226..9245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9305..9321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9305..9321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9318..9340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9329..9345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9353..9359,9360..9377,9378..9383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9398..9419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9536..9553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9707..10295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9787..9806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10466..11257,11258..11326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11089..11108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11327..11431)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
      901 gctgttcgtg gaaatgcagc atcaacggca gcagcaagag gaccacgcga acgaggcaaa
      961 ctatgcaaga ggcaccagac tccctatttc tggtgaagga ccaacttctc agccgaacag
     1021 ctccaagcaa accgtcctgt cttggcaagc tgcaattgat gctgctagac aggccaaggc
     1081 cgcccagacc atgagcacgt ctgcaccccc acctgtagga tctctctccc aaagaaaacg
     1141 tcagcaatac gccaagagca aaaaacaggg taactcgtct aacagccgac ctgcccgtgc
     1201 cctcttctgt ttatccctca ataaccccat ccgaagagcc tgcattagta tagtggattg
     1261 gaaaccattt gacatattta tattattggc tatttttgcc aattgtgtgg ccttagctat
     1321 ttacatcccg ttccctgaag atgattctaa ttcaacaaat cataacttgg aaaaagtaga
     1381 atatgccttc ctgattatct ttacagtgga gacatttctg aagattatag cgtacggact
     1441 gctgctgcat cctaatgctt acgttaggaa cggatggaac ttactggatt ttgttatagt
     1501 aatagtagga ttgtttagtg taattttgga acaattaacc aaagaaacag aaggcgggaa
     1561 ccactctagt ggcaaatcag gaggctttga tgtcaaagcc ctccgtgcct tccgagtgtt
     1621 aagaccgctt cgacttgtat catcagtgcc cagtttacaa gttgttctga actccattat
     1681 aaaagccatg gtccccctcc tccacatagc ccttttggtg ttattcgtaa ttatcatcta
     1741 tgcgattata gggttggaac tttttattgg aaaaatgcac aaaacctgtt tttttgctga
     1801 ctcagatata gtagctgaag aggacccagc tccatgcgcg ttctcaggga atggacgcca
     1861 gtgtgccgcc aatggcacgg aatgtaggag cggctgggtc ggccccaatg gaggcatcac
     1921 taattttgac aactttgcct ttgccatgct cactgtgttc cagtgcatca ccatggaggg
     1981 ctggacagat gtgctctact gggttaatga tgcgatagga tgggagtggc catgggtgta
     2041 ttttgttagt ctgatcatcc ttggctcatt tttcgtcctt aacctggttc ttggtgtcct
     2101 tagtggagaa ttctcaaagg aaagagagaa ggctaaagca cgtggagatt tccagaagct
     2161 tcgggagaag cagcagctgg aagaggatct caagggctac ttggactgga tcacccaggc
     2221 agaagacata gatcctgaga atgaagagga gggtggagag gagggcaaac gaaacactag
     2281 catgcccacc agtgagaccg aatctgtgaa cacagagaac gtaagtggtg aaggcgagac
     2341 ccagggatgc tgtggaagtc tctggtgctg gtggaaaaga agaggcgcgg ccaagactgg
     2401 gccctctggg tgtcggcggt ggggccaagc catctcgaaa tccaagctca gccgtcggtg
     2461 gcgtcgctgg aaccggttca atcgcaggag atgcagggca gctgtgaagt ctgtcacgtt
     2521 ttactggctg gtgatcgttc tggtgtttct caacactctg acgatttcct ctgagcacta
     2581 caatcagcca gactggctga cgcagattca agatatagcg aacaaagtgc tcttggcttt
     2641 gttcacctgt gagatgctgg tgaagatgta cagcctgggc ctccaggcct acttcgtctc
     2701 tctcttcaac cgcttcgatt gctttgtggt gtgtggggga atcaccgaaa ccatcctggt
     2761 ggaactggag ctcatgtctc ccctgggcgt ctctgtgttc cggtgtgtac gtctcctgag
     2821 gatcttcaaa gtgaccaggc actggacttc cctgagcaac ttggtggcgt ccctgttaaa
     2881 ctccatgaag tccatcgcct ctctgctgtt gctgctcttc ctcttcatca tcatcttctc
     2941 cctgctgggg atgcagctgt tcggagggaa gttcaatttc gatgagacac agaccaagcg
     3001 aagcaccttc gacaacttcc cacaggcgct cctgactgtg ttccagatcc tgacgggcga
     3061 agactggaat gcggtgatgt atgacggcat catggcctac ggaggcccgt cctcctctgg
     3121 aatgattgtc tgtatttact tcatcatcct tttcatctgt ggtaactata tcctgctgaa
     3181 cgtcttcttg gccattgctg tagacaattt ggctgatgct gaaagtctga acactgctca
     3241 gaaagaggaa gccgaagaaa aagaaaggaa aaaaattgcc agaaaagaaa gcctagaaaa
     3301 caaaaaaaac aacaaaccag aggtcaacca gatagccaac agtgacaaca aggttactat
     3361 tgatgactat caagaggagg ctgaagataa ggacccttat ccaccctgcg atgtgccagt
     3421 aggtgaagag gaagaggagg aggaagagga cgagcctgag gttcctgctg gtccccgtcc
     3481 tcgaagaatc tcagagttga acatgaagga gaaaattgcg cccatccccg aaggaagcgc
     3541 attcttcatt cttagcaaga ccaacccgat ccgtgtgggt tgccacaaac tcatcaacca
     3601 ccacatcttc accaacctca tcctggtctt catcatgctg agcagcgctg ccctggccgc
     3661 agaggatccc atccgcagcc actccttccg caacactata ctgggctact ttgactatgc
     3721 tttcacagcc atctttacgg ttgaaatcct gttaaagatg acaacttttg gagcgttctt
     3781 gcacaaaggg gctttctgta ggaactactt caatttgctg gacatgctgg tcgttggggt
     3841 gtctctggtg tcatttggga ttcaatccag tgccatctcg gttgtgaaga ttctgagggt
     3901 cttaagggtc ttgaggcctc tcagagcaat caacagagca aaaggactta agcacgtggt
     3961 ccagtgtgtc tttgtggcca tccgaaccat cggcaacatc atgatcgtca cgaccctgct
     4021 ccagttcatg tttgcttgca ttggggtcca gctgttcaag gggaagttct accgttgcac
     4081 agatgaggcc aaaagtaacc ccgaggagtg cagggggctt ttcatccttt ataaggacgg
     4141 cgatgtcgac agtcccgtgg tccgtgagag gatctggcaa aacagtgatt tcaatttcga
     4201 caatgtcctt tcggctatga tggtgctctt cacggtctcg acttttgagg gctggcccgc
     4261 gttgctgtac aaagctattg actcaaacgg agagaacgtt ggtcctgtct acaactaccg
     4321 tgtggagatc tccatcttct tcattatcta catcatcatc gtggccttct tcatgatgaa
     4381 tatcttcgtg ggcttcgtca tcgtcacctt ccaggagcag ggagaaaagg agtataagaa
     4441 ctgtgagctg gacaaaaatc agcgtcagtg tgtggaatat gccttgaagg cccgcccctt
     4501 aaggagatac atccccaaaa acccatacca gtacaagttc tggtacgtgg tgaactcctc
     4561 gcctttcgaa tatatgatgt ttgtcctcat catgctcaac acgctctgcc tggccatgca
     4621 gcactatgag caatccaaga tgttcaatga cgccatggac attctgaaca tggtcttcac
     4681 gggggtcttc accgttgaga tggttttgaa agtcatcgca tttaagccca agcactattt
     4741 cactgatgca tggaatactt ttgatgcctt aattgttgtc ggtagcgtcg ttgatattgc
     4801 tataactgaa gtgaataact ctgaagagag caatagaatc tccatcacct ttttccgtct
     4861 tttccgagtg atgcggttgg tgaagcttct cagcagaggg gaaggcatcc ggactctgct
     4921 atggaccttc attaagtcct tccaggcact cccatatgtc gccctcctca ttgccatgct
     4981 cttcttcatc tacgccgtca ttggcatgca gatgtttggg aaggttgcca tgagagataa
     5041 caaccagatc aataggaaca acaacttcca gacgtttccc caggcagtgc tgctgctctt
     5101 caggtgtgca acaggggagg cctggcagga gatcatgctc gcctgcctcc ctgggaagct
     5161 gtgtgacccg gactcagatt acaacccagg agaggaatat acttgtggga gcaactttgc
     5221 cattgtctac ttcatcagct tttacatgct ctgtgcgttc ctgatcatca acctcttcgt
     5281 ggctgtcatc atggacaatt ttgactatct gacgagggac tggtctattc tggggcctca
     5341 ccacttggac gaattcaaaa gaatatggtc tgaatatgac cccgaggcaa agggaaggat
     5401 aaaacacctt gatgtggtca ctttgctccg acgtatccag cctcccctgg gatttggaaa
     5461 gttgtgtcca caccgagtgg cgtgtaagag attggtcgcc atgaacatgc ctctcaacag
     5521 tgatgggaca gtcatgttca atgcaactct gtttgctttg gtacggacgg ctctcaagat
     5581 caagactgaa ggcaacctgg agcaagctaa tgaagagctc cgcgctgtga taaagaaaat
     5641 ctggaagaag acaagcatga agctacttga ccaagttgtc cctccagctg gtgatgatga
     5701 ggtaaccgtg gggaagttct atgccacttt cctgatacag gactacttta ggaaattcaa
     5761 gaagcggaaa gagcaaggcc tggtggggaa ataccctgcg aagaacacca cgatcgccct
     5821 acaggcggga ttaaggaccc tgcatgacat tgggccagaa atccgacggg ctatatcctg
     5881 tgacttgcaa gatgacgaac cagaagactc caaaccagaa gaggaagatg tattcaaaag
     5941 aaatggtgcc ctgcttggaa actatgtcaa tcatgttaat agtgatagga gagagtccct
     6001 tcagcagacc aataccaccc accgtcccct gcatgtccaa aggccttcaa ttccacctgc
     6061 aagtgatact gagaaaccgc tgtttcctcc agcaggaaat tcggtgtgtc ataaccatca
     6121 taaccataat tccataggga agcaagttcc cacctccaca aatgccaatc tcaataacgc
     6181 caatatgtcc aaagctgccc acggaaagcg gcccagcatt ggggaccttg agcatgtgtc
     6241 tgaaaacggc cattactcct acaagcacga ccgagagctt caaagaaggt ccagtattaa
     6301 aagaacccgc tattatgaaa cttacattag gtctgagtca ggagacgagc agcttccaac
     6361 catttgccgt gaagaccctg agatacacgg ctacttccga gacccccgct gcttcgggga
     6421 gcaggaatat ttcagtagcg aggagtgctg tgaggatgac agttctccca cctggagcag
     6481 gcaaaactac agctattaca acaggtaccc aggcagctcc atggactttg agaggccccg
     6541 aggctaccat cacccccaag gtttcttaga agatgacgac tctcccattg gctatgactc
     6601 acggagatct ccaagaagac gccttctacc tcctacccca ccatcccatc ggaggtcctc
     6661 cttcaacttt gagtgtctgc gcaggcaaaa cagccaagat gacgtccttc cgtcgcctgc
     6721 cctgcctcac cgcgctgccc tgcccctgca ccttatgcag cagcagatca tggccgttgc
     6781 aggcctagat tccagtaaag cccagaagta ctcgccgagc cactctaccc ggtcatgggc
     6841 cacccctcca gcaacccctc cgtaccgaga ctggacccca tgctacaccc ctttgatcca
     6901 ggtggatcgc tcagagtcta tggaccaggt caatggcagc ctgccgtcgc tacaccgcag
     6961 ttcctggtat acagatgaac ccgacatctc ctataggact ttcacaccgg ccagcctgac
     7021 cgtccccagc agcttccgga acaaaaacag cgacaagcag aggagtgcag acagcctggt
     7081 ggaagcagtc ctgatatccg aaggcttggg acgctacgca agggacccaa aatttgcctc
     7141 agcaacaaaa catgagatcg ccgatgcctg tgacctaacc atagacgaga tggagagcgc
     7201 ggccagcacc ctgctcaatg gaagcgtgtg tcctcgagcc aacggggaca tgggccccat
     7261 ctcacaccgc caggactatg agctccagga ctttggtcct ggctacagtg acgaggaacc
     7321 agaccctggg cgagaggaag aggacctggc agatgaaatg atttgcatca ccaccttgta
     7381 gcaatcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     7441 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     7501 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7561 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7621 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7681 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7741 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7981 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8101 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8161 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8461 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     8521 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     8581 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     8641 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     8701 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     8761 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     8821 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     8881 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     8941 tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg
     9001 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9061 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9121 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9241 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9301 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10201 ttggtagctc ttgatccggc aaaaaaacca ccgctggtag cggtggtttt tttgtttgca
    10261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
//
