LOCUS       sequence-215247-        4020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Brent AE, Schweitzer R, Tabin CJ
  TITLE     A somitic compartment of tendon progenitors.
  JOURNAL   Cell. 2003 Apr 18. 113(2):235-48.
  PUBMED    12705871
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Apr 18. 113(2):235-48."
COMMENT     Sequence Label: pBS mPax1 (CT#1007)
FEATURES             Location/Qualifiers
     source          1..4020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2179..2195)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2180..2196)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2220..2239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2221..2239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2246..2263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2246..2262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2255..2277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2404..2859
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2491..2510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2701..2722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2885..2989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2990..3058,3059..3850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3208..3227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gactaggagg
     1081 tcgaggctgg gatgggcagt ccgtgtaagc taccgagtgc atccgtggcc ttgcctcccg
     1141 ggctgggggg cttccggtcg gtcccttctc ggtgtttgaa ggtcattgcc gctgcaagct
     1201 ccccgccgtg caccgccaca cctgctccgt ggggcgtcag tgggggtgcc tgggctggtg
     1261 gccagggcgg gccgggagat aggtagccgg ctgctggagc gctgtatact ccgtgctggt
     1321 tggaagccgg gtaggcgcag gcaggaagga agcctcccac tgcggagagg ctggaagccg
     1381 actgagtata tttaatgtct gcctccaagg caggtttctc gagtccattc actgctggcg
     1441 aggtggggaa ggctgcacgg ttcacacccg cccagtcttc catcttgggg gagtaggcag
     1501 cgccttcgct cccggccagg gcccctgttt gctccataaa cgtccggatg cccaggatgt
     1561 tgctgactga atgtgcggag ggccatgaac gcgggatact gacgtggccc gccgagccgg
     1621 ggaccccggg atgagtgccc atcttagtgc ccgtgggtga cactgggctg gggtaggggt
     1681 actggtagat atgattgtag ggcagtgcag gctgcggagg cggctgctta cttgcttcat
     1741 atggccctgg ctgcgccagg ctgccaatct tatttcgcag gatgcgactg atggagctca
     1801 cagagggcac gttgtacttg tcacaaacgc catcagccag tagccggtca cggatctccc
     1861 aggcaaagat gccaggatcc ccctgtttgt agtcccggat gtgcttcact acattggggg
     1921 tggtaactcg aggtttgctg cccccgatgg ccccgggcag gatggagccg gtctcgttgt
     1981 agcgcgccag gattttgctc acgcagccat gagagacccg cagttgccta ctgatgtcac
     2041 agggtcggat acccagctgt gctagctcca cgattcgtag gcggatggca ttgggcaggg
     2101 gacggccgtt gacgaacacg ccaccaagtt ggttcacttc gccgtacgtc tgctccatga
     2161 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     2221 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2281 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2341 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2401 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2461 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2521 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2581 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2641 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2701 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2761 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2821 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2881 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2941 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3001 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3061 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3121 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3181 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3241 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3301 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3361 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3421 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3481 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3541 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3601 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3661 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3721 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3781 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3841 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3961 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
