LOCUS       sequence-215248-       18630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector: the coding sequence of M71 is replaced by the 
            sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP 
            followed by ACNF cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Katidou M, Grosmaitre X, Lin J, Mombaerts P
  TITLE     G-protein coupled receptors Mc4r and Drd1a can serve as surrogate 
            odorant receptors in mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2018 Apr;88:138-147. doi: 
            10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31.
  PUBMED    29407371
REFERENCE   2  (bases 1 to 18630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2018-04"; volume: "88:138-147. doi"; pages: " 
            10.1016/j.mcn.2018.01.010. Epub 2018 Jan 31"
COMMENT     Sequence Label: Mc4r->M71-IRES-tauGFP ACNF TV
FEATURES             Location/Qualifiers
     source          1..18630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(116..184,185..976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(334..353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1147..1735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1636..1655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1889..1906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2023..2044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2059..2064,2065..2082,2083..2089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2097..2113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2102..2124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2121..2137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2121..2137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2158..2176
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2237
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(4244..4261)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7776..8327
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7920..7937)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      9503..9512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9509..9511,9512..9514,9515..10228)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9509..9511,9512..9514,9515..10225)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9815..9834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10162..10183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    10275..10308
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    10335..10383
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(10390..11193)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10501..10520)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11111..11130
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    11931..11979
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          12329..12394
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(13220..13240)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13264..13275)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    13950..13983
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(17935..17951)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17936..17952)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(17976..17995)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(17977..17995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18002..18019)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18002..18018)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18011..18033)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      18160..18615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18247..18266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     18457..18478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
       61 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      181 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      241 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      301 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      361 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      421 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      481 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      541 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      601 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      661 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
      721 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
      781 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
      841 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      901 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
      961 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1261 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1921 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1981 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2041 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     2101 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
     2161 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gcgagctcaa
     2221 ttaaccctca ctaaagggag tcgactcgat caacatgcgg atacttcatt cctccttaga
     2281 atagggaaca aaataccaat ggaaggagtt acagagacaa agtttggagc taagacaaaa
     2341 ggatagatca tccagagact actccaccca ggggtccatc ccataatcag ccaccaaatg
     2401 cagacactat tgcacatgcc accaagattt tgctgaaagg accctgatat agctgtctct
     2461 tgtgaggcta cgccagtgcc tggcaaatac agaagtggat gctcacagtc atctattgga
     2521 tggaacacag ggcccccaat gaaggagcta gagaaagaac ccaaggaact gaaggggtct
     2581 gcaaccctat aggttgaaca acaatataaa ctaaccagta cccccagagc tcgtgactct
     2641 agctgcatat gtagcagagg atggcctagt cagccatcat tgggaagaga ggccccttga
     2701 tcttgcaaac tttatatgcc ccagtacagg gcaactccag ggccaagaag tgggagtggg
     2761 tgggtagggg agcagggcgg gggggggggt gtatagggaa ctttcaggat agcatttgaa
     2821 atgtaaataa agaaaatatc taataaaatt taaaaaaaaa aagaaaagaa aactgtccca
     2881 gaaagaacaa taaaaatatt ctattaaact tgccagactt ctcttttaag aaggctataa
     2941 gggggaaagt gtagaaaaca gacacagaga aacaagatta actgacaatg tataataaca
     3001 ctcatgtgta atggcactga aaatgatgtt gcaggtagtc actgggtacc tataggaatg
     3061 aaaataatga taatgatgat acattggtag taatttgtag tactgatttc ctgtcagaaa
     3121 agatctggaa acaatgatct tccagaagca atgagcttct ctggcatcag attcacattc
     3181 ctggggtgtc atttccctac tgaaagaacc aagattcttg gtgaaatgaa tgactcagct
     3241 ctgaagaatg aaaagaatgt ggtaaagtca caacatcttg tcataccaga tggtaaggaa
     3301 gatattagag actagtatat taagttcaga gacaaattga gaagtccaga gactgtcaat
     3361 acctggggca gtagcttact ggttaagtgt gcgcactgct gttgctgagc acctgagtgt
     3421 ggttctcagc acatagctca acttataact caaggtccaa gggggaattt attgctgtcc
     3481 cctccaaagg tatttgtgtt catgtgaaca taccaacaca gacacatgta caatttaaaa
     3541 taatgaaaat aaaatgtaaa acataaaatt aaattctgat aaaagcatag taattgtatt
     3601 gggctgaagc acattaaata tgttcacact agtgaattta taaagggggg gatataagaa
     3661 accaactatt gttttaaaat tataaaacat atctttttat tataactgta atagcaaata
     3721 caggaactat tctgttgaat agatagcaca aaaataagca ccaaaatgag tgtgattaaa
     3781 attggagagc tctgaataaa attaaaacta gtgtcaatat tctggccatg atgagtacag
     3841 ctgttttcaa agacattact ctctccccgg gaaggaattt tagcaacagg cttccacttt
     3901 gtgcttcaag ttaaaattgc aaaacaaaga agccaggacc tgaatttgag aaataatgtg
     3961 tgtcatttgt ctttctgtcc ctgagttgcc tcacttggca aaactttgtt tcaacaaaac
     4021 tctttaaaac aagcatgtca aggctagctc atgatgcatc atggtgacta gctaacaata
     4081 tgtaataacc atcatggcac tgagaatgat gttgctggta gttactgtgg tgcctattga
     4141 gatgaaaatg acaataatga taatacatca gtagtaaagg tgatagccat ggtcataatg
     4201 gtaatgatga taaggatggg tgggtagtgg tgatggatgg ttggtggtgg tggtggtgat
     4261 ggtagtggta ggggggtaat ggtggtggta acggctctgt tgatgctaaa ttgttcattg
     4321 ccccattata ttctaagttt ctgaaactga aagatgactt ttacagataa aagaagaatt
     4381 aacacacttg ggaaataaaa catgattcac agaacaagag aaaacatgaa ctaattgtta
     4441 ctttagagac aacaatggtc tctagagtga ctatatccca ggagatgatc cacacacaca
     4501 cacacatata tataaacaga acccccaatt tttagtcata ttatatcacc tttccaaatg
     4561 acaatctcct tttccggact agactgggct ctttaagtaa attttgtggt gtatacacat
     4621 ctgcatactt ctagaattta ctgaatattg aagccacaga cattgcctga agtttcattt
     4681 gagacgctgt ttcatttcct tcagcacagg tattttgtct aaaaattaga tgagaaaata
     4741 tcttctcttg tgagctcttc tttatagttt agactattta tgaccatatg ttgacagtta
     4801 acttttaatg ctctgttcta tgtgatgtac tctcttcaga aaaaatcctg agcttctgaa
     4861 agcttgataa taatagctaa tacacatagg aactcagaag gtatgtgtgg tcctagttgg
     4921 tcttttatat cattagaaaa tttaagctgt acctgatagc taacactgct acctgtattt
     4981 tcagcacttg gtcagctaag gcaggagaat cattacaagc tgaaggttaa cctgggcttt
     5041 ggagtgagat cttgtctcaa aaaacagtag tgggagacct ggagaggtgg ctcagcagtt
     5101 aagagcactg actgctcatc cagaggtcat gagttcaaat cccaaaaacc atctgtaatg
     5161 agatctgatg ccctcttctg gtgtatctga agacagcaac agcaacagtg tattcataag
     5221 cataaaataa ataaatacat ctttaaaaag aatcagtagt ggggaaaaag acaagaataa
     5281 aaagctgcag aagaagatta tataagcaaa aaaaaaaatt gagactagct gtctgagctc
     5341 attctacaag aatgtattct catctacatt gcactgggta tataacattt accctgacct
     5401 gatttacaaa gtattttaat ctctgctaat ggttactgaa tatgtttctt ataatattaa
     5461 gaagcagaga ttccataaag agggataggt aatattttta attattcatc atctagataa
     5521 aaatcatttt gtagccgggc ttgtggcaca cgcctttaat cccagcactt gggaggcaga
     5581 ggcaggcaga tttctgagtt caaggccagc ctggtctaca aggtgagttc caggacagcc
     5641 agggctatac agagaaactc tgtctcaaaa attaaaaaaa gatcattttg tagtaaataa
     5701 aaataatatt caagatcata ccgatctctg atatgtcaat atgtaacttt agttaagaaa
     5761 cgttctatca ctaattcaat atgtaaaccc tacatataac acatatgttc taatattcaa
     5821 aatattccaa agatgttaaa gattctctta tacttaagaa aaaggaagtc acttgactca
     5881 gtagatgaag ccattttcca tcaaatctta aaacttgagt tttatcccta gaagctttaa
     5941 gaaacaagga gaaagccaat ttctgcaagt tgtatataca cacacaacac agcacagcac
     6001 actcactata cacaaaatct cttttcacaa aactggtact ataagtgact tttgaaagaa
     6061 tgaactgaag atgtgtcaat aattaataat tgtatcaaaa acaattacca tagtgagatt
     6121 cctgctatgc cttaagaaat gtatcccata ctgtacaggg gcagcacatg tctgcaatcc
     6181 cagccagcag gaagaagaag aaggggtaag ttcaaggaaa accaatgcta catagaaaga
     6241 cttaactttc aaaaaaagaa aaagaaaaat agaaagaaat agagaataat acctcagata
     6301 tgagaattta atttaatatg tatcacatct actaataaca acacatgctt cttaggaaaa
     6361 tatactatat atgtatatat aatatgtatg atttgacata gtgataaatg gtgcaatggt
     6421 ttaggatcta aaaataatag ctaaaattaa caacaaagtg cttgctgatc ccacaataag
     6481 caactactca tcaaatatga caggacagca gtaagaaata cagaacttgg ccctttacta
     6541 agctttaagg cctctgaaac cctaagggct ggtccttctg tcagcaagaa gcaacagtat
     6601 agagaagata tgtgtgccac agccagaata gctcagcaag tgtcaaatgc agaaaaccag
     6661 ctctccaagc agggcaagaa cagccattca caaactcctt ctctcccacc acacaggctt
     6721 cccacccaga cacaatgaac tccacccacc accatggcat gtatacttcc ctccacctct
     6781 ggaaccgcag cagctacggg ctgcacggca atgccagcga gtcgctgggg aagggccacc
     6841 cggacggagg atgctatgag caactttttg tttcccccga ggtgtttgtg actctgggtg
     6901 tcataagcct gttggagaac attctagtga tcgtggcgat agccaagaac aagaacctgc
     6961 actcacccat gtactttttc atctgtagcc tggctgtggc agatatgctg gtgagcgttt
     7021 cgaatgggtc ggaaaccatc gtcattaccc tgttaaacag tacggatacg gatgcccaga
     7081 gcttcaccgt gaacattgat aatgtcattg actctgtgat ctgtagctcc ttgctcgcat
     7141 ccatttgcag cctgctttcc attgcggtgg acaggtattt cactatcttt tacgcgctcc
     7201 agtaccataa catcatgacg gttaggcggg tcgggatcat cataagttgt atctgggcag
     7261 cttgcactgt gtcaggcgtc ctcttcatca tttactcgga cagcagcgct gtcatcatct
     7321 gcctcatttc catgttcttc actatgctag ttctcatggc ctctctctat gtccacatgt
     7381 tcctgatggc gaggcttcac attaagagga ttgctgtcct cccaggcaca gggaccatcc
     7441 gccagggtac caacatgaag ggggcgatta ccttgaccat cctgattgga gtctttgttg
     7501 tctgctgggc cccgttcttt ctccatttac tgttctacat ctcttgccct cagaatccat
     7561 actgcgtgtg cttcatgtct cattttaatt tgtatctcat actgatcatg tgtaacgccg
     7621 tcatcgaccc tctcatttat gccctccgga gtcaagaact gaggaaaact ttcaaagaga
     7681 tcatctgttt ctatcctctg ggaggcatct gtgagttgtc tagcaggtat taatgtggcg
     7741 cgccttaatt aagaattccg cctccccccc ccctaacgtt actggccgaa gccgcttgga
     7801 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     7861 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     7921 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     7981 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     8041 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     8101 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctaagc
     8161 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     8221 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     8281 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     8341 ggctgagccc cgccaggagt tcgacgtgat ggaagatcat gctcaggggg actacaccct
     8401 gcaagaccag gagggtgaca tggaccccgg cctgaaagag tctcccctgc agaccccggc
     8461 cgatgatgga tctgaggaac caggctctga aacctctgat gctaagagca ctccgacggc
     8521 ggaagatgcg acagcaccct tagtggatga gggagccccc ggtgagcagg cggccgctca
     8581 ggcccccgcg gagatcccag aaggaaccgc agctgaagaa gcaggcattg gcgacacgtc
     8641 caacctggaa gaccaagctg ccggacacgt gacccaagct cgcatggtca gtaaaggcaa
     8701 agatgggact ggacccgatg acaaaaaaac caagggggcg gatggtaagc ctggaacgaa
     8761 gattgccaca ccccggggag cagcccctcc aggccagaaa ggccaggcca acgccacccg
     8821 gattccagca aaaaccactc ccaccccgaa gacctcgcca ggtgaatctg ggaaatccgg
     8881 ggaccgcagc ggctacagca gccccggctc cccaggcact ccgggcagcc gctcccgcac
     8941 accctccctg ccgaccccgc ccacccggga gcccaagaag gtggcggtgg tccgcactcc
     9001 ccccaagtcg ccgtctgcag ccaagagccg cctgcaggcc gctcccgggc ccatgccaga
     9061 cctgaagaac gtcaagtcca aaatcggctc cacggaaaac ctgaagcacc agccaggagg
     9121 tggcaaggtg cagataatta ataagaagct ggatcttagc aacgtccagt ccaagtgtgg
     9181 ctcaaaggat aatatcaaac acgtgccagg aggcggcagt gtgcaaatag tctacaaacc
     9241 agtggatctg agcaaggtga cctccaagtg tggctcatta ggcaacatcc atcataagcc
     9301 aggaggtggc caagtggaag tgaaatctga gaagctggac ttcaaggata gagtccagtc
     9361 gaagattggg tccctggaca acatcacaca cgtccctggc ggagggaata aaaagatcga
     9421 aacccacaag ctgaccttcc gcgagaacgc caaagccaag accgaccacg gggcggagat
     9481 cgtgtccggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     9541 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     9601 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     9661 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     9721 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     9781 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     9841 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     9901 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     9961 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
    10021 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    10081 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
    10141 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
    10201 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagagatt cgagatctag
    10261 atatcgatga attcataact tcgtataatg tatgctatac gaagttatgg atctgtcgat
    10321 cgacggatcg atccgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg
    10381 ccgatcccct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
    10441 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
    10501 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
    10561 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
    10621 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
    10681 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
    10741 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
    10801 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
    10861 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
    10921 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
    10981 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
    11041 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
    11101 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
    11161 gtgcaatcca tcttgttcaa tggccgatcc catattggct gcacggatcc tgaacggcag
    11221 aggttacggc agtttgtctc tcccccttcc gggagccacc ttcttctcca accgtcccgg
    11281 tcgcgctctc ggcgcttctg aggagagaac tggctgagtg acgcccttta tagattcgcc
    11341 cttgtgtccc gccccttcct ttcccgccct cccttgcgct acggggccgc ccgcaccggc
    11401 ctacacggag cgcgcgcggc ggagttgttg acgctagggc tccggctccc tggttgggtg
    11461 ttctttctga cgcgacagga ggaggagaat gttcctggtc ctgtcgtcct cctttcgggt
    11521 ttcccgtgca ctcaaaccga ggacttacag aacggaggat aaagttaggc catttttact
    11581 cagcttcgga gttcaggctc attttcagct aaagtctctc attagtatcc ccccacacac
    11641 atcgggaaaa tggtttgtcc tacgcatcgg taatgaaggc ggggcccttc gggtcctccg
    11701 gagcgggttc cgggggtggg gggaaggagg gagggacggg acgggcctcg ttcatgaata
    11761 ttcagttcac cgctgaatat gcataaggca ggcaagatgg cgcgtccaat caattggaag
    11821 tagccgttat tagtggagag gccccaggac gttggggcac cgcctgtgct ctagtagctt
    11881 tacggagccc tggcgctcga tgttcagccc aagctttcgc gagctcgacc gaacaaacga
    11941 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgc tcagctttac agtgacaatg
    12001 acggctggcg actgaatatt agtgcttaca gacagcacta catattttcc gtcgatgttg
    12061 aaatcctttc tcatatgtca ccataaatat caaataatta tagcaatcat ttacgcgtta
    12121 atggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac
    12181 ggatatagtt catgacaata tttacattgg tccagccacc agcttgcatg atctccggta
    12241 ttgaaactcc agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc
    12301 aggccaggta tctctgacca gagtcatcct aaaatacaca aacaattaga atcagtagtt
    12361 taacacatta tacacttaaa aattttatat ttaccttagc gccgtaaatc aatcgatgag
    12421 ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg gctggtggca gatggcgcgg
    12481 caacaccatt ttttctgacc cggcaaaaca ggtagttatt cggatcatca gctacaccag
    12541 agacggaaat ccatcgctcg accagtttag ttacccccag gctaagtgcc ttctctacac
    12601 ctgcggtgct aaccagcgtt ttcgttctgc caatatggat taacattctc ccaccgtcag
    12661 tacgtgagat atctttaacc ctgatcctgg caatttcggc tatacgtaac agggtgttat
    12721 aagcaatccc cagaaatgcc agattacgta tatcctggca gcgatcgcta ttttccatga
    12781 gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac
    12841 cggcatcaac gttttctttt cggatccgcc gcataaccag tgaaacagca ttgctgtcac
    12901 ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga
    12961 tagtttttac tgccagaccg cgcgcctgaa gatatagaag ataatcgcga acatcttcag
    13021 gttctgcggg aaaccatttc cggttattca acttgcacca tgccgcccac gaccggcaaa
    13081 cggacagaag cattttccag gtatgctcag aaaacgcctg gcgatccctg aacatgtcca
    13141 tcaggttctt gcgaacctca tcactcgttg catcgaccgg taatgcaggc aaattttggt
    13201 gtacggtcag taaattggac accttcctct tcttcttggg catggccgca ggaaagcaga
    13261 gccctgaagc tcccatcacc ggccaataag agccaagcct gcagtgtgac ctcatagagc
    13321 aatgtgccag ccagcctgac cccaagggcc ctcaggcttg ggcacactgt ctctaggacc
    13381 ctgagagaaa gacataccca tttctgctta gggccctgag gatgagccca ggggtggctt
    13441 ggcactgaag caaaggacac tggggctcag ctggcagcaa agtgaccagg atgctgaggc
    13501 tttgacccag aagccagagg ccagaggcca ggacttctct tggtcccagt ccaccctcac
    13561 tcagagcttt accaatgccc tctggatagt tgtcgggtaa cggtggacgc cactgattct
    13621 ctggccagcc taggacttcg ccattccgct gattctgctc ttccagccac tggctgaccg
    13681 gttggaagta ctccagcagt gccttggcat ccagggcatc tgagcctacc aggtccttca
    13741 gtacctcctg ccagggcctg gagcagccag cctgcaacac ctgcctgcca agcagagtga
    13801 ccactgtggg cacaggggac acagggtggg gcccacaaca gcaccattgt ccacttgtcc
    13861 ctcactagta aaagaactct agggttgcgg ggggtggggg aggtctctgt gaggctggta
    13921 agggatattt gcctggccca tggagatcca taacttcgta taatgtatgc tatacgaagt
    13981 tataagcttt cgcgagctcg aatcggatcc gaattcttaa ttaaggtacc ggcgcgcctt
    14041 taattaaaaa tgctgatctt tcttcatgta aaagtaagtg gttatgcaca cctgtatgat
    14101 aacctctgca agctcaccca actctgaaca ggaaacagga atcattgctc tccattgttg
    14161 ggctcaaggc caatctcctt gtcctcattc tcatcttctc taccattggt tctatttata
    14221 attactcgat tttaagataa tttttatatt attgggtctc tgtcctctat atgaggccaa
    14281 tggtaagcaa ttttataatt tttactatta atatttcatc tattatataa tgtatttgat
    14341 catatccacc ccaccctctt cttctctttc attatcaccc cctatatatc tacctcccat
    14401 tttcatgtcc tctttttttt ttaataacca actgcatcca attaatactg tttatattca
    14461 cattagtgtc tggccatgca tgggagcatg ggtaacctac tagcaaatct cacacaaatt
    14521 catttaatta gtggaatcta aattacttct agggtactag ttgcaagtga ttctgagaaa
    14581 tgcagtttcc agcttgttga cactaaatag aatgtgtatt aaagggttag taggacagtc
    14641 tcacttaaat agctttttac tcattttctt ctgacctttc cccaacaaaa gaagaaattg
    14701 ctaccactgt tgatatgctc taattagaac taaaggtagg aaactatctc agagggagac
    14761 ctctcgatgc aacaacaaca acaacaacaa caaaaaggca aaagtgtaat tctgagttag
    14821 aatacatgcc tgaagagggt gaggcactga atttgacgtg atgtaatcag tttcacaatt
    14881 tacttatatt ggttttaaat attgtaatct gcaaagtggg caaaaccagg gaaagataat
    14941 tctgtgttcc agacaagaca gaacaatcag atccaaatct gtagagcagg ttatttccac
    15001 agaaacatag tgcttctcag ttcaagctgc tcgcccaaat aaagctctgg agaggcgact
    15061 tgtgaagcat ggaacacctc actagactgc ccatggattt tagcccaaga aaagtctttg
    15121 tcaccagagc taggacattt agttttattt cctggttagt catgaatttt ccctcttaaa
    15181 ctttaccttt tcatagagtt gactccacta tacttagaac cacaaactct cactgtcaac
    15241 aacagcatcc tcattgtgaa taccttccat ggaaagacca gaagtctttt ctctttagcc
    15301 ttgactttac ttcagtttat tacaagcaca aatgtatttt tttcattatt tggaattgat
    15361 caatttgatg gcattgtgca aattgcttta ccaattggat gaattttctc aaaagtgtag
    15421 tttttaaaga gaagtttatt ttgaagatct ttatgaaact gcctataata gagaggagct
    15481 gtggcagact aaacaccaaa aatctttcct ctgcctttgt gctaaaactg ttgtttctaa
    15541 cacacgcaca caaaaatctc caacagtagg cgtacataat ctggcataca tcactgctct
    15601 taaaaacttt ttaaactgcc gggcagtggt ggtgcatgtc tttaatccca gcatttggga
    15661 agcagaggca ggcaaatttc tgagttcaaa gccagcctgg tctacagaga gagttccaga
    15721 acagcccagg ttatacaacg aaaccctgtc tcaaataaat aaataaataa ataaataaat
    15781 aaataacctt ttaaactaat gttagtatct tcatgatctc aaactgattt aggaaaataa
    15841 cgtgtccttc tttagtgttt ggtatagaga gtataggctc ttcactaaat atttacagga
    15901 atcctttgtg aataacttca aagtatacaa ggccattcct aaatttataa gcttgcacta
    15961 tagatttttg ttgaactgta gtcacacatc ttgtggtttt caaaaatagt atatctaact
    16021 tatgaaatgt attattagcc cttttcttct tttccccttc ttttctcatg actgaaggta
    16081 tgaagccagt tcaatgatgt gttcatctgt gtactaaata ctgatagtgt ttgtgtgcac
    16141 aaaggctaaa aaggcaaaaa tttttggaag aagagtttgc tctacctgaa acctgtaaaa
    16201 tacagtcttt aaatgactat gtaacagtga aaggttcttt tgtactaagc acttagaagg
    16261 tgagtgacaa caatgtagga gaaaactaat ttaaggtcat ttccagtcct tcctcttagc
    16321 cactcaagag tacaactgtc catcactcct aaaatgtcca ccctcttgaa catctgtgaa
    16381 attccctttt tcccttaatt accttctaaa tagtctcaat tcctcacata taccttctat
    16441 tttatttttt gcttctggtc ttcactagga cactgtgtct gacagtttgg ccttgcccac
    16501 cacattcaca tagctgtcga gtaatggctc cacatcaccc aagccttccc cagcatctcc
    16561 actgtcaaca agctgatgcc ttttatggac atgttaaggg aaactttcag cagtgatttt
    16621 tttgtaggtt ttcatctaag ggttaggtct tatgaagttt cctcaatcta catttatcag
    16681 ctgatgtcat tacacaggtc ttgttgaggt tgtcacaatt ttataggtgc agcttcttcg
    16741 ttgccatatc caaagatgct atctcatagc aggtagccgg ttcctctagc tcttataatc
    16801 tttccaccac tcttccatga tgatccctga gacttaaata tagtgtctac atgtagttgc
    16861 gaatatctgc catagtgtca gatatgaatc ccctcctagt tagcaagcct taagactttg
    16921 aggttgttgc cattccctag ctactgtgag taaagtggaa atgaacacag tcaagcaagt
    16981 atctgttgag gagaacaccc agtcctttgg caatatacca aagaagaata cagctagatc
    17041 ctgtgataat ccagttataa ctttctgtga atcctccaca gtgattacca tagaggcttc
    17101 cctatttgca accctagcaa caatcaataa gtctttttgc ctttctctga atcctagaca
    17161 tttgtttcag ttgttttctt aatcctaacc atctgactgg agtaaggcaa aatctaaaag
    17221 cagttttaat ttgcattttc atagttgcaa aggaagttaa acacattttt acatatttgt
    17281 cagccatttt tatttcttcc tctgagatct ttctcttcaa atccagggcc tattaggggt
    17341 ggggagtggt cagttaatga ccttaatgac tgagcaaatg gagtctttct cagaaagtct
    17401 tttactacat ctctatctta gaggggactg cctgtgtact cttcaagtgg ttttagcatt
    17461 taacgttgcc catggaagtt tttgatccat ttggaatttt tgtaccaaat aatagatgtg
    17521 ggactatttt tattcttcta catgtggaca tccagtttta ccagcacaat ttgttgaaga
    17581 gtttgtcttt ataccagtgt atatgtttaa catttttgtc aaatatcaga tggctaaact
    17641 catgtttcag ttttctgttc tgttactctg atttaaatgc ctgttggttt ggccatttat
    17701 tttgctttgt tttgttgcca gtaccatgca atttttatta ctatagctct ataacactgg
    17761 ttgaaactgt gttctatctc tctagcattg ttctttctgc tcaggatttc tttggttatc
    17821 cttagtctct tatggttcca tatggatttt aggacagttt tattatttct gtgaagatgt
    17881 tgtggagatt tttagtggga attaatgttt aaacgaattc gatatcaagc ttatcgatac
    17941 cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacaatt
    18001 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    18061 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    18121 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
    18181 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    18241 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    18301 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    18361 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    18421 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    18481 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    18541 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    18601 taacgcttac aatttaggtg gcacttttcg
//
