LOCUS       sequence-215257-        9948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of 6His-dCas9-3XFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Tsui C, Inouye C, Levy M, Lu A, Florens L, Washburn MP, Tjian R
  TITLE     dCas9-targeted locus-specific protein isolation method identifies 
            histone gene regulators.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2734-E2741. doi: 
            10.1073/pnas.1718844115. Epub 2018 Mar 5.
  PUBMED    29507191
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1718844115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-03-20- 20"; volume: "115"; issue: "12"; pages: 
            "E2734-E2741"
COMMENT     Sequence Label: pCT310
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             93..110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             120..143
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(153..176,177..179,180..2666,2667..2669,2670..4253)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4278..4343
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(4441..4459)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4455..4502
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4740..4758)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4826..4930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4931..4999,5000..5791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5149..5168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5962..6550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6451..6470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6704..6721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6736..6875
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6801..6820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6961..6983
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6977..7168)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8480..9562)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9524..9543
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9563..9640)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9846..9865
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaaact ttaagaagga gatatacata tgcatcatca tcatcatcac gtgggaggtc
      121 tggaagttct gttccagggg cccgcagcct cggataagaa atactcaata ggcttagcta
      181 tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt ccgtctaaaa
      241 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
      301 ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa
      361 ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg
      421 cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca
      481 agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga
      541 aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt
      601 tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg
      661 agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa
      721 cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga
      781 ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg
      841 gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta
      901 attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt
      961 acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt
     1021 tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga gtaaatactg
     1081 aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
     1141 acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct
     1201 tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag
     1261 aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg
     1321 tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
     1381 cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc
     1441 catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt
     1501 atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag
     1561 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
     1621 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac
     1681 atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta
     1741 ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt
     1801 tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa
     1861 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat
     1921 taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag
     1981 aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga
     2041 tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc
     2101 ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta
     2161 gggataagca atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc
     2221 gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag
     2281 cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc
     2341 ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa
     2401 tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc
     2461 aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat
     2521 taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
     2581 tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta
     2641 atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt aaagacgatt
     2701 caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg gataacgttc
     2761 caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt
     2821 taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac
     2881 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
     2941 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc
     3001 gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc
     3061 aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg tatctaaatg
     3121 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg
     3181 gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca
     3241 aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
     3301 cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag
     3361 aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc
     3421 aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt
     3481 taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat
     3541 atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct aaggtggaaa
     3601 aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca attatggaaa
     3661 gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat aaggaagtta
     3721 aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa aacggtcgta
     3781 aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct ctgccaagca
     3841 aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt agtccagaag
     3901 ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat gagattattg
     3961 agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta gataaagttc
     4021 ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa aatattattc
     4081 atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt gatacaacaa
     4141 ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt atccatcaat
     4201 ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt gacggtggct
     4261 ccagatctgc ggccgcggac tacaaagacc atgacggtga ttataaagat catgacatcg
     4321 attacaagga tgacgatgac aagtagggcc gctgactcga gatcgatgat attcgagcct
     4381 aggtataatc ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     4441 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     4501 tgctgaaagg aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac
     4561 caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt
     4621 agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta
     4681 aagctagctt atcgatgata agctgtcaaa catgagaatt aattcttgaa gacgaaaggg
     4741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5161 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5461 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6721 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6781 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     6841 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     6901 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6961 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     7021 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     7081 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     7141 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     7201 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     7261 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     7321 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     7381 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     7441 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     7501 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     7561 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     7621 tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg
     7681 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
     7741 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
     7801 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
     7861 aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc
     7921 ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag
     7981 cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     8041 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     8101 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat
     8161 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     8221 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     8281 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     8341 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     8401 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     8461 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8521 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     8581 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     8641 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     8701 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     8761 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     8821 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     8881 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     8941 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     9001 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     9061 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     9121 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     9181 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     9241 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     9301 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     9361 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     9421 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     9481 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     9541 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     9601 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
     9661 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     9721 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     9781 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     9841 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     9901 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
//
