LOCUS       sequence-21527-e         216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E,
            Carr SA, Sabatini DM
  TITLE     PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein 
            kinase.
  JOURNAL   Mol Cell. 2007 Mar 23. 25(6):903-15.
  PUBMED    17386266
REFERENCE   2  (bases 1 to 216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Mar 23. 25(6):903-15."
COMMENT     Sequence Label: pRK5 Flag PRAS40
FEATURES             Location/Qualifiers
     source          1..216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgtccactcc caggtccaac tgcacctcgg ttctatcgat tgaattcacc atggaaatgg
       61 actacaagga tgacgatgac aagggtggtg gtggtggtgc gtcgacgatg gcgtcggggc
      121 gccccgagga gctgtgggag gccgtggtgg gggccgctga gcgcttccgg gcccggactg
      181 gcgcggagct ggtgctgctg accgcggccc cgccgc
//
