LOCUS       sequence-215281-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid, responsive to activated Ah receptor/Arnt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Miller CA 3rd
  TITLE     A human aryl hydrocarbon receptor signaling pathway constructed in 
            yeast displays additive responses to ligand mixtures.
  JOURNAL   Toxicol Appl Pharmacol. 1999 Nov 1;160(3):297-303. doi: 
            10.1006/taap.1999.8769.
  PUBMED    10544064
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/taap.1999";
            journalName: "Toxicol Appl Pharmacol"; date: "1999-11-1- 1"; volume:
            "160"; issue: "3"; pages: "297-303"
COMMENT     Sequence Label: pTXRE5-Z
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3433..3449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3433..3449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3446..3468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3457..3473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3481..3487,3488..3505,3506..3511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3526..3547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3664..3681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3835..4423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3915..3934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4594..5385,5386..5454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5217..5236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5455..5559)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5596..6099
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6140..6158
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6196..6218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6318..6337
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6358..6638
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6639..7313
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7416..7871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7553..7574)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7765..7784
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7997..8019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8011..8028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8012..8028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8035..8054
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8035..8053
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(8206..8393)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8314..8338
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 atgaccggat ccggagcttg gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
       61 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      121 cgacaggttt cccgacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      241 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
      301 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
      361 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
      421 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
      481 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
      541 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
      601 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
      661 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
      721 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
      781 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
      841 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
      901 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
      961 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
     1021 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
     1081 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
     1141 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
     1201 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     1261 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     1321 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     1381 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     1441 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
     1501 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
     1561 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
     1621 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
     1681 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
     1741 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
     1801 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
     1861 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
     1921 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
     1981 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
     2041 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
     2101 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
     2161 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
     2221 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg
     2281 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt
     2341 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg
     2401 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
     2461 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
     2521 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc
     2581 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
     2641 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat
     2701 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc
     2761 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
     2821 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
     2881 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
     2941 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
     3001 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
     3061 tggagcccgt cagtatcggc ggaattacag ctgagcgccg gtcgctacca ttaccagttg
     3121 gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg
     3181 aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt attctttttc
     3241 ttttactttt ttatcatggg agcctacttc ccgtttttcc cgatttggct acatgacatc
     3301 aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct gttctcgcta
     3361 ttattccaac cgctgtttgg tctgctttct gacaaactcg gcctcgactc tagcgaatta
     3421 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5581 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
     5641 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     5701 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     5761 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     5821 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     5881 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     5941 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     6001 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     6061 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa ccattattat
     6121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcacg
     6361 acattactat atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta
     6421 ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt
     6481 gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt
     6541 cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt
     6601 gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag
     6661 gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg
     6721 ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa
     6781 ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag
     6841 gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc
     6901 tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat
     6961 accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca
     7021 tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag
     7081 caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag
     7141 agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg
     7201 atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg
     7261 gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta
     7321 ctgagtagta tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac
     7381 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     7441 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7501 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7561 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7621 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7681 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7741 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7801 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7861 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     7921 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     7981 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     8041 gactcactat agggcgaatt gggatccgtg tggaagaacg attacaacag gtgttgtcct
     8101 ctgaggacat aaaatacaca ccgagattca tcaactcatt gctggagtta gcatatctac
     8161 aattgggtga aatggggagc gatttgcagg catttgctcg gcatgccggt agaggtgtgg
     8221 tcaataagag cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta
     8281 ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt
     8341 atttttttta taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta
     8401 gaagtgtcaa caacgtatct accaacgatt tgaccctttt ccatcttttc gtaaatttct
     8461 ggcaaggtag acaagccgac aaccttgatt ggagacttga ccaaacctct ggcgaagaag
     8521 tccaaagctc caccgcggtg gcggccgtta cttacttaga gctcgacgtc ttacttactt
     8581 agcggccgca ctagtagatc cttgcgtgac aattcttgcg tgacaattct tgcgtgacaa
     8641 ttcttgcgtg agaattcttg cgtgacaatg tcgacccggg gtcgacctcg aggcatgtgc
     8701 tctgtatgta tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat
     8761 taggtccttt gtagcataaa ttactatact tctatagaca cgcaaacaca aatacacaca
     8821 ctaaattaat a
//
