LOCUS       sequence-215300-        9350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Induces expression of human MICB allele 005 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Ferrari de Andrade L, Tay RE, Pan D, Luoma AM, Ito Y, Badrinath S, 
            Tsoucas D, Franz B, May KF Jr, Harvey CJ, Kobold S, Pyrdol JW, Yoon 
            C, Yuan GC, Hodi FS, Dranoff G, Wucherpfennig KW
  TITLE     Antibody-mediated inhibition of MICA and MICB shedding promotes NK 
            cell-driven tumor immunity.
  JOURNAL   Science. 2018 Mar 30;359(6383):1537-1542. doi: 
            10.1126/science.aao0505.
  PUBMED    29599246
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-03-30- 30"; volume: "359"; 
            issue: "6383"; pages: "1537-1542"
COMMENT     Sequence Label: pHAGE-EF1a-MICB*005-IRES-ZsGreen
FEATURES             Location/Qualifiers
     source          1..9350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4450..5017
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4467..5019
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4611..4628)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4838..4857
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5031..5726
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    5740..6328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5793..5813)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6211..6222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6403..6636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6755..6773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6841..6945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6946..7014,7015..7806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7164..7183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7977..8565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8466..8485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8719..8736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8976..8997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9012..9017,9018..9035,9036..9042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9050..9066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9055..9077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9074..9090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9074..9090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9140..9159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgggg
     3421 ctgggccggg tcctgctgtt tctggccgtc gccttccctt ttgcaccccc ggcagccgcc
     3481 gctgagcccc acagtcttcg ttacaacctc atggtgctgt cccaggatgg atctgtgcag
     3541 tcagggtttc tcgctgaggg acatctggat ggtcagccct tcctgcgcta tgacaggcag
     3601 aaacgcaggg caaagcccca gggacagtgg gcagaagatg tcctgggagc taagacctgg
     3661 gacacagaga ccgaggactt gacagagaat gggcaagacc tcaggaggac cctgactcat
     3721 atcaaggacc agaaaggagg cttgcattcc ctccaggaga ttagggtctg tgagatccat
     3781 gaagacagca gcaccagggg ctcccggcat ttctactacg atggggagct cttcctctcc
     3841 caaaacctgg agactcaaga atcgacagtg ccccagtcct ccagagctca gaccttggct
     3901 atgaacgtca caaatttctg gaaggaagat gccatgaaga ccaagacaca ctatcgcgct
     3961 atgcaggcag actgcctgca gaaactacag cgatatctga aatccggggt ggccatcagg
     4021 agaacagtgc cccccatggt gaatgtcacc tgcagcgagg tctcagaggg caacatcacc
     4081 gtgacatgca gggcttccag cttctatccc cggaatatca cactgacctg gcgtcaggat
     4141 ggggtatctt tgagccacaa cacccagcag tggggggatg tcctgcctga tgggaatgga
     4201 acctaccaga cctgggtggc caccaggatt cgccaaggag aggagcagag gttcacctgc
     4261 tacatggaac acagcgggaa tcatggcact caccctgtgc cctctgggaa ggcgctggtg
     4321 cttcagagtc aacggacaga ctttccatat gtttctgctg ctatgccatg ttttgttatt
     4381 attattattc tctgtgtccc ttgttgcaag aagaaaacat cagcggcaga gggtccatag
     4441 acgcgtggat ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4501 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4561 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4621 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4681 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4741 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4801 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4861 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4921 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4981 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat atggcccagt
     5041 ccaagcacgg cctgaccaag gagatgacca tgaagtaccg catggagggc tgcgtggacg
     5101 gccacaagtt cgtgatcacc ggcgagggca tcggctaccc cttcaagggc aagcaggcca
     5161 tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg
     5221 ccttcatgta cggcaaccgc gtgttcaccg agtaccccca ggacatcgtc gactacttca
     5281 agaactcctg ccccgccggc tacacctggg accgctcctt cctgttcgag gacggcgccg
     5341 tgtgcatctg caacgccgac atcaccgtga gcgtggagga gaactgcatg taccacgagt
     5401 ccaagttcta cggcgtgaac ttccccgccg acggccccgt gatgaagaag atgaccgaca
     5461 actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg
     5521 acgtgagcat gtacctgctg ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg
     5581 tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg gcacttcatc cagcacaagc
     5641 tgacccgcga ggaccgcagc gacgccaaga accagaagtg gcacctgacc gagcacgcca
     5701 tcgcctccgg ctccgccttg ccctgaatcg atagatccta atcaacctct ggattacaaa
     5761 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5821 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5881 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5941 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6001 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6061 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6121 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6181 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6241 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6301 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     6361 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6421 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6661 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     6721 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     6781 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     7201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
     8821 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8881 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
     8941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9001 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9061 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
     9121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9181 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     9241 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     9301 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
