LOCUS       sequence-215302-       12968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 
            bidirectional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12968)
  AUTHORS   Miller CA 3rd
  TITLE     A human aryl hydrocarbon receptor signaling pathway constructed in 
            yeast displays additive responses to ligand mixtures.
  JOURNAL   Toxicol Appl Pharmacol. 1999 Nov 1;160(3):297-303. doi: 
            10.1006/taap.1999.8769.
  PUBMED    10544064
REFERENCE   2  (bases 1 to 12968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/taap.1999";
            journalName: "Toxicol Appl Pharmacol"; date: "1999-11-1- 1"; volume:
            "160"; issue: "3"; pages: "297-303"
COMMENT     Sequence Label: pIGAhRC
FEATURES             Location/Qualifiers
     source          1..12968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..665
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    217..334
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     629..652
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(4284..4303)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             5504..6307
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             6939..7130
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7124..7146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7232..7372
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7287..7306
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7387..7404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7558..8146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7638..7657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8317..9108,9109..9177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8940..8959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9178..9282)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9350..9368
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     regulatory      12942..12951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttcaaaaat tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca
       61 tggcattacc accatataca tatccatata catatccata tctaatctta cttatatgtt
      121 gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca
      181 gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt
      241 gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct
      301 gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc
      361 ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa
      421 cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg
      481 gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact
      541 gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat
      601 gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa
      661 aaaacccggg gagtagccgc cgccgtcggc tgggcaccat gaacagcagc agcgccaaca
      721 tcacctacgc cagtcgcaag cggcggaagc cggtgcagaa aacagtaaag ccaatcccag
      781 ctgaaggaat caagtcaaat ccttccaagc ggcatagaga ccgacttaat acagagttgg
      841 accgtttggc tagcctgctg cctttcccac aagatgttat taataagttg gacaaacttt
      901 cagttcttag gctcagcgtc agttacctga gagccaagag cttctttgat gttgcattaa
      961 aatcctcccc tactgaaaga aacggaggcc aggataactg tagagcagca aatttcagag
     1021 aaggcctgaa cttacaagaa ggagaattct tattacaggc tctgaatggc tttgtattag
     1081 ttgtcactac agatgctttg gtcttttatg cttcttctac tatacaagat tatctagggt
     1141 ttcagcagtc tgatgtcata catcagagtg tatatgaact tatccatacc gaagaccgag
     1201 ctgaatttca gcgtcagcta cactgggcat taaatccttc tcagtgtaca gagtctggac
     1261 aaggaattga agaagccact ggtctccccc agacagtagt ctgttataac ccagaccaga
     1321 ttcctccaga aaactctcct ttaatggaga ggtgcttcat atgtcgtcta aggtgtctgc
     1381 tggataattc atctggtttt ctggcaatga atttccaagg gaagttaaag tatcttcatg
     1441 gacagaaaaa gaaagggaaa gatggatcaa tacttccacc tcagttggct ttgtttgcga
     1501 tagctactcc acttcagcca ccatccatac ttgaaatccg gaccaaaaat tttatcttta
     1561 gaaccaaaca caaactagac ttcacaccta ttggttgtga tgccaaagga agaattgttt
     1621 taggatatac tgaagcagag ctgtgcacga gaggctcagg ttatcagttt attcatgcag
     1681 ctgatatgct ttattgtgcc gagtcccata tccgaatgat taagactgga gaaagtggca
     1741 tgatagtttt ccggcttctt acaaaaaaca accgatggac ttgggtccag tctaatgcac
     1801 gcctgcttta taaaaatgga agaccagatt atatcattgt aactcagaga ccactaacag
     1861 atgaggaagg aacagagcat ttacgaaaac gaaatacgaa gttgcctttt atgtttacca
     1921 ctggagaagc tgtgttgtat gaggcaacca acccttttcc tgccataatg gatcccttac
     1981 cactaaggac taaaaatggc actagtggaa aagactctgc taccacatcc actctaagca
     2041 aggactctct caatcctagt tccctcctgg ctgccatgat gcaacaagat gagtctattt
     2101 atctctatcc tgcttcaagt acttcaagta ctgcaccttt tgaaaacaac tttttcaacg
     2161 aatctatgaa tgaatgcaga aattggcaag ataatactgc accgatggga aatgatacta
     2221 tcctgaaaca tgagcaaatt gaccagcctc aggatgtgaa ctcatttgct ggaggtcacc
     2281 cagggctctt tcaagatagt aaaaacagtg acttgtacag cataatgaaa aacctaggca
     2341 ttgattttga agacatcaga cacatgcaga atgaaaaatt tttcagaaat gatttttctg
     2401 gtgaggttga cttcagagac attgacttaa cggatgaaat cctgacgtat gtccaagatt
     2461 ctttaagtaa gtctcccttc ataccttcag attatcaaca gcaacagtcc ttggctctga
     2521 actcaagctg tatggtacag gaacacctac atctagaaca gcaacagcaa catcaccaaa
     2581 agcaagtagt agtggagcca cagcaacagc tgtgtcagaa gatgaagcac atgcaagtta
     2641 atggcatgtt tgaaaattgg aactctaacc aattcgtgcc tttcaattgt ccacagcaag
     2701 acccacaaca atataatgtc tttacagact tacatgggat cagtcaagag ttcccctaca
     2761 aatctgaaat ggattctatg ccttatacac agaactttat ttcctgtaat cagcctgtat
     2821 taccacaaca ttccaaatgt acagagctgg actaccctat ggggagtttt gaaccatccc
     2881 cataccccac tacttctagt ttagaagatt ttgtcacttg tttacaactt cctgaaaacc
     2941 aaaagcatgg attaaatcca cagtcagcca taataactcc tcagacatgt tatgctgggg
     3001 ccgtgtcgat gtatcagtgc cagccagaac ctcagcacac ccacgtgggt cagatgcagt
     3061 acaatccagt actgccaggc caacaggcat ttttaaacaa gtttcagaat ggagttttaa
     3121 atgaaacata tccagctgaa ttaaataaca taaataacac tcagactacc acacatcttc
     3181 agccacttca tcatccgtca gaagccagac cttttcctga tttgacatcc agtggattcc
     3241 tgtaattcca agcccaattt tgaccctggt ttttggatta aattagtttg tgaaggatta
     3301 tggaaaaata aaactgtcac tgttggacgt cagcaagttc acatgaagat ctgtcatgat
     3361 gatcattgca attggatcca tatatagggc ccgggtaccg agcgaagatc tgtcatgatg
     3421 atcattgcaa ttggatccat atatagggcc cgggtaccga gctccaaaga aggctctgaa
     3481 atgcttattt ttttcaagcg ataccaagta aacagttaca tacctgaatg atactaccac
     3541 aactatttac attatcgtcc tgtcagaagg cgaataatca gaactgtttg ttaacatgtc
     3601 attttataag agcggcctga aatactgtca taagtggtct gtcgaaagta gttattacgg
     3661 cgtcgggcgt cgcgaatagc tcaaaagtca ccccagctta cacgataatc ccataaccta
     3721 cagcaagaag ctattcttgg atacaagcac cagttacaag cactgcattc atatattgcc
     3781 ttttactttc ctattctgta tttgtgtgct aagtgtcctg tttcggaacg ttctagaaga
     3841 ttttcttatt tttctctttt tcagtttttt tttcatcacg caaaggaaat acaatacaag
     3901 gttaaaatga aggaactaac attactatta tttccaattt aaagtttacc ttaaaagaca
     3961 atagattgat cttgaaagcc agtttagata gattttagaa caaacagaaa agtaaacatt
     4021 aaaataaaaa tgagtatccc cgctgacatt gcatctttaa ttagtgacaa gtacaaaagt
     4081 gccttcgata atggtaactt aaaatttatc cagactgaaa caacgaaaac aaaggaccca
     4141 aaaaccagca tgccatactt gattagccac atgccaagtc tgatcgaaaa gccagagcgt
     4201 ggccaaactc cagaaggaga ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
     4261 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     4321 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     4381 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     4441 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     4501 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     4561 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     4621 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     4681 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     4741 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     4801 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     4861 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     4921 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     4981 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     5041 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     5101 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     5161 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     5221 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat cggggggggg
     5281 gggggggggg aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa
     5341 tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag
     5401 attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac
     5461 ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata
     5521 taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca
     5581 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
     5641 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
     5701 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     5761 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     5821 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     5881 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     5941 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
     6001 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
     6061 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     6121 agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac
     6181 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     6241 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     6301 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     6361 aatttaatta tatcagttat tacccgggaa tctcggtcgt aatgattttt ataatgacga
     6421 aaaaaaaaaa attggaaaga aaaccccccc cccgcagcgt tgggtcctgg ccacgggtgc
     6481 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
     6541 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
     6601 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
     6661 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     6721 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     6781 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     6841 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     6901 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     6961 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     7021 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     7081 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7201 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7261 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7321 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     7381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     8641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     8941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9301 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9361 cacgaggccc tttcgtcttc aagaattcct gcagcatcta ccgttgatct agatgctctg
     9421 cttcttctac caggggtcga gtcccacgac ggcgttgata ctgtattttc gaccaaagac
     9481 ttttatcaag tgtcattcgt caaatccatt gctcctgcta tcgtaaacag ctccgtaatc
     9541 ttccacgatg tttctcgtgg tgtggctatg ggcaatgtca agagcagagc aagtatcttc
     9601 aacccagagg aaacgtatta cgattgggaa cagtaccaag tagtaaataa cggagactgg
     9661 cgaaccgaat gggcacctgc ctctaactgc atttggaggg aggagaagga taacagcgac
     9721 gaaacaccgg acagattccc catctcggtg ccatataatt ggacgtcaca gtactcaatt
     9781 gtagattatg acacagacgc taacgaagac aatttagatt tcaggtttat taaatcattg
     9841 ctagataaga aaaattggtt gaataaaatt aaccagactg tttcccaatc cagtattatg
     9901 gtagcaccaa tgattaagcc atacaatgtg gtccagcttt ggtattcaaa atatatggtt
     9961 tgggcaaacg ttcaaagaca atattgtagc ggtgtttatc caggagggac tcaatgtagc
    10021 gcttggtcca ggtactacca tgttgatgca cctacctgcg atgagcctgt cgcctcttac
    10081 atgaccaaaa tgtcggaaaa tgaggttcag tgtcccaatg agagaaacgc aactacccta
    10141 gagcctctcc gcctgaataa gcagggagac tctgattttt ctttgacttt cgaggaagag
    10201 gaagaggaag agacaggatc taaatctctt tggagtacat tgaaaaaaat tttctctaaa
    10261 agaagtataa gttgatctta gttggtagca ctttgatgag gtctctagca aaaagtctac
    10321 aaacaaattc ttgaaagtgt ttgattgtat atagcttgca tgcctgcagg tcgactctag
    10381 aggatccggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc tttttttttt
    10441 tttttataaa tgttacctta gagaggcaac agagcagggg aacagccaga agggaaaggg
    10501 ggtacatgtc aggggtgagg gaagggaagg gagaggaact tttattctgt ttacagaaag
    10561 atttgctttt taaaaacaaa cagtgatttt ttctccccca ccccttatcc tcaccccaat
    10621 agttctattc tgaaaagggg ggaaacatag ttagatcagg gaattcttca ttgttgtagc
    10681 tgttgctctg atctcccagc atggacagca tctcctggaa gacctcaggc tggccaggtt
    10741 gctgtgctgg cggttgttga acatgttgct cactagaact tgaacgatga tgaggctgct
    10801 ggccctgcca ctgtggccag acacccacac cctctgctgt ccgtgtctgg aattgtcctg
    10861 cagtctgtcc agtctcagga gcaaagttag atccacgatt ggtgagacta gggtaggcag
    10921 cagcaccagg cgatgcagtt ggggcaccag ggagggacat ggagctgaag gaggatggag
    10981 tctgaaagct gcccacacca aactgggaag tacgagtctt agcagtagcc tgggtagcca
    11041 cctgctgggc agaaaagcct gagcgggtag taggggtcca agttggggtt gctccttggg
    11101 tggggttgga gtggcgggaa atctgggcca acatctgtcc tgcagaagct gatggctgga
    11161 caatggttac aggaggggct aggccactat tcctgaaatt ctctgccggc cggggggtag
    11221 gagggaatgt gttgccctgg gagaatagct gttgggtggc agggacagtg ctggaggaga
    11281 tgcctttact ctgatccgca ttgatgttgt gatagatttc tgaaaatctt ggatctctat
    11341 cctgggcaaa taaaccatct gacttctcaa ggggcttgct gtgttctggt cctgtggttg
    11401 tcacaggctg aaccacctgg gaatgattgt agctggccag tccatctctt cctggtacca
    11461 tgtccaattc tgtttgctgt tgctgctgcc tgggtgccag ctgtcctgag cccatctcca
    11521 ggggtaaatt agctgtggga cctagttgtg gcctctggat tgtgttggag agtgtaggcc
    11581 gtggttcttg gctagagttc ttcacattgg tgttggtaca gatgatgtac tcaatttcat
    11641 ctgagtaagg gttctggaaa gtaaaggagc tggttctcat ccagagccat tcttggttct
    11701 tagaccggaa ccggaacatg acagacagca cttggccttt taatttcact acctgttgga
    11761 agctgtctct tagaagctgc tggtcttcag gatgacagaa ttctacaata ttctttccta
    11821 agagttcctg tggctggtag ccaacagtag ccacacagcg gtgatccaca aaagtgaaga
    11881 taccctcaat gttgtgtcgg gagatgaact ctgttggttg acaaacatta ctcatgtctg
    11941 tacagttggg agaactagtt acctgcaatc tgccaatggc cactaggcaa aacttgcttc
    12001 cctggccagc ctctgggtca tcatctggga gggaaacacc tgctgggggc caggccctga
    12061 tgtagcctgt gcagtggacc accacgaagt gaggttcccc atcctttaca gagccaagtc
    12121 cattcctgca tctgttcctc acaaagctca gcctattcac agaaactggg tccacagagc
    12181 tactgccaca cctcattcgg caaataaacg atctccttga gcccatacac attctcatgg
    12241 aagactgctg accttccttt ttcactgttc cagtctttag atccaggata cgccctgtca
    12301 gggcattttc tgaagtggaa agctgctcac gaagtttatc cacatcatct gggtgcacct
    12361 gatcatagag tgtgctgcca aaccattcag actgtggctg gttcaaaaca ggagtcacgg
    12421 agtcagacac atacaccacc ctgcctgtct cacatgagac aataaacaga aagccatctg
    12481 ctgcctccaa gatcaaatgt ttcagttcct gatcagtgag gaaagacggc ttataggagc
    12541 catcagtgga tgtgttgcca gttccccgca aggacttcat gtgagaaact gccatgcgta
    12601 agatggttag cttgtctggt tttcgagcca gggcactaca ggtgggtacc atatctgaca
    12661 gttctgtgat gtaggctgtc atcttgttcc gtcgccgccg ttcaatttca ctgtgatttt
    12721 ccctggcaaa ccgctcctta tcgttagaca tctgatcatc atcacacctc aaaaatttac
    12781 tgttcccttc tccatcatca tcaaaatcca gccctggtcg ccgcttaata gccctctgga
    12841 caatggctcc tccaccttga attccaggtc cagagtttcc agaggcaatg gctggaccca
    12901 gtgatggtac atctgatgtc atttcggggt tggcagtagt cgccgccatg gccgcagatg
    12961 ggatcccc
//
