LOCUS       sequence-215323-       11536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty sgRNA expression vector with co-expression of TurboGFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Hulton CH, Costa EA, Shah NS, Quintanal-Villalonga A, Heller G, de 
            Stanchina E, Rudin CM, Poirier JT
  TITLE     Direct genome editing of patient-derived xenografts using 
            CRISPR-Cas9 enables rapid in vivo functional genomics.
  JOURNAL   Nat Cancer. 2020 Mar;1(3):359-369. doi: 10.1038/s43018-020-0040-8. 
            Epub 2020 Mar 9.
  PUBMED    33345196
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-020-0040-8"; journalName: "Nat Cancer"; date: 
            "2020-03"; volume: "1"; issue: "3"; pages: "359-369"
COMMENT     Sequence Label: sgTrack-GFP
FEATURES             Location/Qualifiers
     source          1..11536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    235..360
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    853..1086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1271..1315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1613..1730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..2029
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1781..1801
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1952..1971
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3911..3986
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4048..4259
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    4319..4343
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4346..5044
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     protein_bind    complement(5046..5070)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5132..5188
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5189..5785
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5682..5702
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5801..6389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5854..5874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6272..6283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6461..6694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6720..6737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6726..6950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6996..7424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7083..7102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7293..7314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7433..7453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7438..7767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7618..7753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7680..7699
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7815..7862
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7881..8255
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8385..8506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8422..8441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8476..8495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8555..8571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8555..8571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8568..8590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8579..8595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8603..8609,8610..8627,8628..8633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8648..8669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8786..8803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8957..9545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9037..9056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9716..10507,10508..10576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10339..10358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10577..10681)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10756..10775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10947..11326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11328..11526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11481..11501
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
       61 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      181 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      241 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      301 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      361 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      421 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      481 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      541 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      601 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      661 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      721 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      781 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      841 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      901 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      961 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1021 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1081 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1141 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1201 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1261 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1321 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1381 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1441 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1501 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1561 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1741 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     1801 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     1861 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     1921 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     1981 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2041 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2101 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2161 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2221 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2281 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2341 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2401 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2461 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     2521 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     2581 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     2641 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     2701 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     2761 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     2821 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     2881 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     2941 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3001 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3061 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3121 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3181 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3241 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3301 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3361 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3421 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3481 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     3541 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     3601 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     3661 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     3721 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     3781 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     3841 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     3901 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     3961 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
     4021 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4081 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     4141 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4201 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4261 accggttcta gaccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac
     4321 aagtttgtac aaaaaagcag gcttaatgga gagcgacgag agcggcctgc ccgccatgga
     4381 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
     4441 agagggcacc cccgagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
     4501 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
     4561 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
     4621 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
     4681 cgaggccggc cgcgtgatcg gcgacttcaa ggtgatgggc accggcttcc ccgaggacag
     4741 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
     4801 gggcgataac gatctggatg gcagcttcac ccgcaccttc agcctgcgcg acggcggcta
     4861 ctacagctcc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
     4921 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gaggatcaca gcaacaccga
     4981 gctgggcatc gtggagtacc agcacgcctt caagaccccg gatgcagatg ccggtgaaga
     5041 ataataccca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag
     5101 tctagagggc ccgcggttcg aaggatccgg cgcaacaaac ttctctctgc tgaaacaagc
     5161 cggagatgtc gaagagaatc ctggaccgac cgagtacaag cccacggtgc gcctcgccac
     5221 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5281 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5341 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5401 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5461 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5521 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc
     5581 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5641 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5701 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5761 catgacccgc aagcccggtg cctgaacgcg ttaagtcgac aatcaacctc tggattacaa
     5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6181 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6361 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     6421 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6481 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6661 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     6721 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6781 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6841 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6901 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6961 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7021 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7081 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7141 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7201 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7261 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7321 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7381 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7441 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7501 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7561 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7621 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7681 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7741 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7801 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7861 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     7921 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7981 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     8041 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     8101 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     8161 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     8221 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     8281 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8341 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8521 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8581 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8641 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8701 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8761 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8821 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8881 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8941 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9001 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9061 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9121 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9181 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9241 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9301 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9361 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9421 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9481 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9541 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9601 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9661 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9721 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9781 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9841 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9901 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9961 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10021 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10081 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10141 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10201 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10261 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10321 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10381 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10441 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10501 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10561 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10621 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10681 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    10741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    10801 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    10861 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
    10921 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
    10981 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    11041 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    11101 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    11161 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    11221 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    11281 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    11341 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    11401 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    11461 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    11521 aagcagcgcg ttttgc
//
