LOCUS       sequence-215326-       11548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty sgRNA expression vector with co-expression of mCherry 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Hulton CH, Costa EA, Shah NS, Quintanal-Villalonga A, Heller G, de 
            Stanchina E, Rudin CM, Poirier JT
  TITLE     Direct genome editing of patient-derived xenografts using 
            CRISPR-Cas9 enables rapid in vivo functional genomics.
  JOURNAL   Nat Cancer. 2020 Mar;1(3):359-369. doi: 10.1038/s43018-020-0040-8. 
            Epub 2020 Mar 9.
  PUBMED    33345196
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-020-0040-8"; journalName: "Nat Cancer"; date: 
            "2020-03"; volume: "1"; issue: "3"; pages: "359-369"
COMMENT     Sequence Label: sgTrack-mCherry
FEATURES             Location/Qualifiers
     source          1..11548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    235..360
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    853..1086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1271..1315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1613..1730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..2029
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1781..1801
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1952..1971
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3911..3986
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4048..4259
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    4319..4343
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4346..5056
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4491..4509)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4759..4778
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5058..5082)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5144..5200
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5201..5797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5694..5714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5813..6401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5866..5886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6284..6295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6473..6706
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6732..6749)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6738..6962
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7008..7436
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7095..7114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7305..7326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7445..7465)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7450..7779
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7630..7765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7692..7711
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7827..7874
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7893..8267
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8397..8518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8434..8453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8488..8507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8567..8583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8567..8583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8580..8602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8591..8607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8615..8621,8622..8639,8640..8645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8660..8681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8798..8815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8969..9557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9049..9068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9728..10519,10520..10588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10351..10370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10589..10693)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10768..10787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10959..11338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11340..11538
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11493..11513
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
       61 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      181 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      241 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      301 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      361 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      421 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      481 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      541 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      601 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      661 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      721 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      781 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      841 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      901 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      961 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1021 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1081 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1141 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1201 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1261 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1321 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1381 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1441 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1501 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1561 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1741 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     1801 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     1861 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     1921 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     1981 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg
     2041 taaatgagca cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca
     2101 accaaaatct tctttttaat aactttagta tcaataatta gaatttttat gttccttttt
     2161 gcaaactttt aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt
     2221 tttcttcacc tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt
     2281 caatcatttt ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat
     2341 tgtctttaac aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa
     2401 atcctgttga tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca
     2461 tgatttcttc ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag
     2521 ctgctttttt tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg
     2581 caccaatatt taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt
     2641 cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc
     2701 ctacatttgt gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa
     2761 ctgttgcaaa atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag
     2821 catcttgttt taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat
     2881 attcattttt aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac
     2941 agattcacca acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac
     3001 agcaaatcat gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata
     3061 cattccatcg atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg
     3121 tgttgctctc aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa
     3181 attcaagttt aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata
     3241 gtctactaat gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga
     3301 agcttgtatt ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc
     3361 ataagcaaaa acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca
     3421 tttaaatcca gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc
     3481 acccaaatca cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa
     3541 gcgttttaga tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa
     3601 tcttacaaaa tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt
     3661 cataataata atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg
     3721 atcaaattct ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc
     3781 ttcaacctca ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc
     3841 tgttccatta actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt
     3901 gtacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     3961 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
     4021 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4081 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     4141 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4201 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4261 accggttcta gaccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac
     4321 aagtttgtac aaaaaagcag gcttaatggt gagcaagggc gaggaggata acatggccat
     4381 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     4441 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4501 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     4561 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     4621 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     4681 gacccaggac tcctccctcc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     4741 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     4801 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     4861 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     4921 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     4981 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     5041 cgagctgtac aagtaatacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     5101 cggccgctcg agtctagagg gcccgcggtt cgaaggatcc ggcgcaacaa acttctctct
     5161 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg accgagtaca agcccacggt
     5221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5401 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5581 cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5761 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttaagtcg acaatcaacc
     5821 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5881 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5941 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6001 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6061 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6121 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6181 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6241 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6301 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6361 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     6421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6481 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     6721 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6781 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6841 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6901 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6961 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7021 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7081 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7141 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7201 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7261 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7321 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7381 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7441 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7501 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7561 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7621 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7681 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7741 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7801 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7861 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7921 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7981 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8041 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     8101 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8161 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8221 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8281 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8341 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8401 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8461 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8521 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8581 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8641 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8701 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9601 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9661 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9721 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9781 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9841 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9901 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9961 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10021 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10081 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10141 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10201 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10261 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10321 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10381 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10441 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10501 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10561 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10681 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10741 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10801 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    10861 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
    10921 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
    10981 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
    11041 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    11101 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    11161 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
    11221 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    11281 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    11341 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
    11401 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    11461 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
    11521 ggaggtctat ataagcagcg cgttttgc
//
