LOCUS       sequence-215345-        3125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transfection in mammalian cells, combination of fluorescent protein 
            fusion and tandem affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Suijkerbuijk SJ, Vleugel M, Teixeira A, Kops GJ
  TITLE     Integration of kinase and phosphatase activities by BUBR1 ensures 
            formation of stable kinetochore-microtubule attachments.
  JOURNAL   Dev Cell. 2012 Oct 16;23(4):745-55. doi: 
            10.1016/j.devcel.2012.09.005.
  PUBMED    23079597
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.09"; journalName: "Dev Cell"; date: 
            "2012-10-16- 16"; volume: "23"; issue: "4"; pages: "745-55"
COMMENT     Sequence Label: pLAP-BUBR1-dKARD
FEATURES             Location/Qualifiers
     source          1..3125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggcgg tgaagaagga agggggtgct ctgagtgaag ccatgtccct ggagggagat
       61 gaatgggaac tgagtaaaga aaatgtacaa cctttaaggc aagggcggat catgtccacg
      121 cttcagggag cactggcaca agaatctgcc tgtaacaata ctcttcagca gcagaaacgg
      181 gcatttgaat atgaaattcg attttacact ggaaatgacc ctctggatgt ttgggatagg
      241 tatatcagct ggacagagca gaactatcct caaggtggga aggagagtaa tatgtcaacg
      301 ttattagaaa gagctgtaga agcactacaa ggagaaaaac gatattatag tgatcctcga
      361 tttctcaatc tctggcttaa attagggcgt ttatgcaatg agcctttgga tatgtacagt
      421 tacttgcaca accaagggat tggtgtttca cttgctcagt tctatatctc atgggcagaa
      481 gaatatgaag ctagagaaaa ctttaggaaa gcagatgcga tatttcagga agggattcaa
      541 cagaaggctg aaccactaga aagactacag tcccagcacc gacaattcca agctcgagtg
      601 tctcggcaaa ctctgttggc acttgagaaa gaagaagagg aggaagtttt tgagtcttct
      661 gtaccacaac gaagcacact agctgaacta aagagcaaag ggaaaaagac agcaagagct
      721 ccaatcatcc gtgtaggagg tgctctcaag gctccaagcc agaacagagg actccaaaat
      781 ccatttcctc aacagatgca aaataatagt agaattactg tttttgatga aaatgctgat
      841 gaggcttcta cagcagagtt gtctaagcct acagtccagc catggatagc accccccatg
      901 cccagggcca aagagaatga gctgcaagca ggcccttgga acacaggcag gtccttggaa
      961 cacaggcctc gtggcaatac agcttcactg atagctgtac ccgctgtgct tcccagtttc
     1021 actccatatg tggaagagac tgcacaacag ccagttatga caccatgtaa aattgaacct
     1081 agtataaacc acatcctaag caccagaaag cctggaaagg aagaaggaga tcctctacaa
     1141 agggttcaga gccatcagca agcatctgag gagaagaaag agaagatgat gtattgtaag
     1201 gagaagattt atgcaggagt aggggaattc tcctttgaag aaattcgggc tgaagttttc
     1261 cggaagaaat taaaagagca aagggaagcc gagctattga ccagtgcaga gaagagagca
     1321 gaaatgcaga aacagattga agagatggag aagaagctaa aagaaatcca aactactcag
     1381 caagaaagaa caggtgatca gcaagaagag acgatgccta caaaggagac aactaaactg
     1441 caaattgctt ccgagtctca gaaaatacca ggaatgactc tatccagttc tgtttgtcaa
     1501 gtaaactgtt gtgccagaga aacttcactt gcggagaaca tttggcagga acaacctcat
     1561 tctaaaggtc ccagtgtacc tttctccatt tttgatgagt ttcttctttc agaaaagaag
     1621 aataaaagtc ctcctgcaga tcccccacga gttttagctc aacgaagacc ccttgcagtt
     1681 ctcaaaacct cagaaagcat cacctcaaat gaagatgtgt ctccagatgt ttgtgatgaa
     1741 tttacaggaa ttgaaccctt gagcgaggat gccattatca caggcttcag aaatgtaaca
     1801 atttgtccta acccagaaga cacttgtgac tttgccagag cagctcgttt tgtatccact
     1861 ccttttcatg agataatgtc cttgaaggat ctcccttctg atcctgagag actgttaccg
     1921 gaagaagatc tagatgtaaa gacctctgag gaccagcaga cagcttgtgg cactatctac
     1981 agtcagactt cctctggctt ctctggttct tctgcctcgg ttgcaagcac ctcctccatc
     2041 aaatgtcttc aaattcctga gaaactagaa cttactaatg agacttcaga aaaccctact
     2101 cagtcaccat ggtgttcaca gtatcgcaga cagctactga agtccctacc agagttaagt
     2161 gcctctgcag agttgtgtat agaagacaga ccaatgccta agttggaaat tgagaaggaa
     2221 attgaattag gtaatgagga ttactgcatt aaacgagaat acctaatatg tgaagattac
     2281 aagttattct gggtggcgcc aagaaactct gcagaattaa cagtaataaa ggtatcttct
     2341 caacctgtcc catgggactt ttatatcaac ctcaagttaa aggaacgttt aaatgaagat
     2401 tttgatcatt tttgcagctg ttatcaatat caagatggct gtattgtttg gcaccaatat
     2461 ataaactgct tcacccttca ggatcttctc caacacagtg aatatattac ccatgaaata
     2521 acagtgttga ttatttataa ccttttgaca atagtggaga tgctacacaa agcagaaata
     2581 gtccatggtg acttgagtcc aaggtgtctg attctcagaa acagaatcca cgatccctat
     2641 gattgtaaca agaacaatca agctttgaag atagtggact tttcctacag tgttgacctt
     2701 agggtgcagc tggatgtttt taccctcagc ggctttcgga ctgtacagat cctggaagga
     2761 caaaagatac tagctaactg ttcttctccc taccaggtag acctgtttgg tatagcagat
     2821 ttagcacatt tactattgtt caaggaacac ctacaggtct tctgggatgg gtccttctgg
     2881 aaacttagcc aaaatatttc tgagctaaaa gatggtgaat tgtggaataa attctttgtg
     2941 cggattctga atgccaatga tgaggccaca gtgtctgttc ttggggagct tgcagcagaa
     3001 atgaatgggg tttttgacac tacattccaa agtcacctga acaaagcctt atggaaggta
     3061 gggaagttaa ctagtcctgg ggctttgctc tttcagtgag cggccgctct agaattcggg
     3121 gatcc
//
