LOCUS       sequence-215346-        3179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transfection in mammalian cells, combination of fluorescent protein 
            fusion and tandem affinity purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Suijkerbuijk SJ, Vleugel M, Teixeira A, Kops GJ
  TITLE     Integration of kinase and phosphatase activities by BUBR1 ensures 
            formation of stable kinetochore-microtubule attachments.
  JOURNAL   Dev Cell. 2012 Oct 16;23(4):745-55. doi: 
            10.1016/j.devcel.2012.09.005.
  PUBMED    23079597
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.09"; journalName: "Dev Cell"; date: 
            "2012-10-16- 16"; volume: "23"; issue: "4"; pages: "745-55"
COMMENT     Sequence Label: pLAP-BUBR1-3A
FEATURES             Location/Qualifiers
     source          1..3179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggcgg tgaagaagga agggggtgct ctgagtgaag ccatgtccct ggagggagat
       61 gaatgggaac tgagtaaaga aaatgtacaa cctttaaggc aagggcggat catgtccacg
      121 cttcagggag cactggcaca agaatctgcc tgtaacaata ctcttcagca gcagaaacgg
      181 gcatttgaat atgaaattcg attttacact ggaaatgacc ctctggatgt ttgggatagg
      241 tatatcagct ggacagagca gaactatcct caaggtggga aggagagtaa tatgtcaacg
      301 ttattagaaa gagctgtaga agcactacaa ggagaaaaac gatattatag tgatcctcga
      361 tttctcaatc tctggcttaa attagggcgt ttatgcaatg agcctttgga tatgtacagt
      421 tacttgcaca accaagggat tggtgtttca cttgctcagt tctatatctc atgggcagaa
      481 gaatatgaag ctagagaaaa ctttaggaaa gcagatgcga tatttcagga agggattcaa
      541 cagaaggctg aaccactaga aagactacag tcccagcacc gacaattcca agctcgagtg
      601 tctcggcaaa ctctgttggc acttgagaaa gaagaagagg aggaagtttt tgagtcttct
      661 gtaccacaac gaagcacact agctgaacta aagagcaaag ggaaaaagac agcaagagct
      721 ccaatcatcc gtgtaggagg tgctctcaag gctccaagcc agaacagagg actccaaaat
      781 ccatttcctc aacagatgca aaataatagt agaattactg tttttgatga aaatgctgat
      841 gaggcttcta cagcagagtt gtctaagcct acagtccagc catggatagc accccccatg
      901 cccagggcca aagagaatga gctgcaagca ggcccttgga acacaggcag gtccttggaa
      961 cacaggcctc gtggcaatac agcttcactg atagctgtac ccgctgtgct tcccagtttc
     1021 actccatatg tggaagagac tgcacaacag ccagttatga caccatgtaa aattgaacct
     1081 agtataaacc acatcctaag caccagaaag cctggaaagg aagaaggaga tcctctacaa
     1141 agggttcaga gccatcagca agcatctgag gagaagaaag agaagatgat gtattgtaag
     1201 gagaagattt atgcaggagt aggggaattc tcctttgaag aaattcgggc tgaagttttc
     1261 cggaagaaat taaaagagca aagggaagcc gagctattga ccagtgcaga gaagagagca
     1321 gaaatgcaga aacagattga agagatggag aagaagctaa aagaaatcca aactactcag
     1381 caagaaagaa caggtgatca gcaagaagag acgatgccta caaaggagac aactaaactg
     1441 caaattgctt ccgagtctca gaaaatacca ggaatgactc tatccagttc tgtttgtcaa
     1501 gtaaactgtt gtgccagaga aacttcactt gcggagaaca tttggcagga acaacctcat
     1561 tctaaaggtc ccagtgtacc tttctccatt tttgatgagt ttcttctttc agaaaagaag
     1621 aataaaagtc ctcctgcaga tcccccacga gttttagctc aacgaagacc ccttgcagtt
     1681 ctcaaaacct cagaaagcat cacctcaaat gaagatgtgt ctccagatgt ttgtgatgaa
     1741 tttacaggaa ttgaaccctt gagcgaggat gccattatca caggcttcag aaatgtaaca
     1801 atttgtccta acccagaaga cacttgtgac tttgccagag cagctcgttt tgtatccact
     1861 ccttttcatg agataatgtc cttgaaggat ctcccttctg atcctgagag actgttaccg
     1921 gaagaagatc tagatgtaaa gacctctgag gaccagcaga cagcttgtgg cactatctac
     1981 agtcagactc tcagcatcaa gaagctggcc ccaattattg aagacgcccg tgaagccgcc
     2041 cactcctctg gcttctctgg ttcttctgcc tcggttgcaa gcacctcctc catcaaatgt
     2101 cttcaaattc ctgagaaact agaacttact aatgagactt cagaaaaccc tactcagtca
     2161 ccatggtgtt cacagtatcg cagacagcta ctgaagtccc taccagagtt aagtgcctct
     2221 gcagagttgt gtatagaaga cagaccaatg cctaagttgg aaattgagaa ggaaattgaa
     2281 ttaggtaatg aggattactg cattaaacga gaatacctaa tatgtgaaga ttacaagtta
     2341 ttctgggtgg cgccaagaaa ctctgcagaa ttaacagtaa taaaggtatc ttctcaacct
     2401 gtcccatggg acttttatat caacctcaag ttaaaggaac gtttaaatga agattttgat
     2461 catttttgca gctgttatca atatcaagat ggctgtattg tttggcacca atatataaac
     2521 tgcttcaccc ttcaggatct tctccaacac agtgaatata ttacccatga aataacagtg
     2581 ttgattattt ataacctttt gacaatagtg gagatgctac acaaagcaga aatagtccat
     2641 ggtgacttga gtccaaggtg tctgattctc agaaacagaa tccacgatcc ctatgattgt
     2701 aacaagaaca atcaagcttt gaagatagtg gacttttcct acagtgttga ccttagggtg
     2761 cagctggatg tttttaccct cagcggcttt cggactgtac agatcctgga aggacaaaag
     2821 atactagcta actgttcttc tccctaccag gtagacctgt ttggtatagc agatttagca
     2881 catttactat tgttcaagga acacctacag gtcttctggg atgggtcctt ctggaaactt
     2941 agccaaaata tttctgagct aaaagatggt gaattgtgga ataaattctt tgtgcggatt
     3001 ctgaatgcca atgatgaggc cacagtgtct gttcttgggg agcttgcagc agaaatgaat
     3061 ggggtttttg acactacatt ccaaagtcac ctgaacaaag ccttatggaa ggtagggaag
     3121 ttaactagtc ctggggcttt gctctttcag tgagcggccg ctctagaatt cggggatcc
//
