LOCUS       sequence-215382-        6958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus 
            expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave
            8xHis-ZZ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: His-ZZ-TEV-BICDN(1-400)-GFP
FEATURES             Location/Qualifiers
     source          1..6958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..165
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     90..107
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             232..255
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             298..471
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             472..645
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             703..723
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             745..765
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1251..1262
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2789..2923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2826..2845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2880..2899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3044..3209
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     3216..3921
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3304..3676
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3333..3921)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4111..4699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4600..4619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     4955..5782
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             4955..5079
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5117..5722)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  5811..6034
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6101..6634)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6823..6828,6829..6845,6846..6851))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(6922..6955)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
       61 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
      121 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
      181 taccgtccca ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat
      241 caccatcacc atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg
      301 gacaacaaat tcaacaaaga acaacaaaac gcgtactatg agatcttaca tttacctaac
      361 ttaaacgaag gacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
      421 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
      481 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      541 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      601 cttttagcag aagctgaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
      661 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
      721 ggagattatg atatccctac gactgagaat ctttattttc agggcatgtc agctccatca
      781 gaagaagagg aatatgctag gttggtcatg gaagcccagc ccgagtggct cagggctgaa
      841 gttaagcgcc tgtctcacga gctcgccgag acaaccagag agaagatcca agcagctgaa
      901 tatggcctcg ctgttttgga ggagaaacac caactgaaac tccagttcga ggagctggag
      961 gttgactacg aggccattag gtcagaaatg gagcaactga aagaagcctt cggccaagca
     1021 cataccaatc ataagaaggt tgccgcagac ggagagtcca gagaagaatc actgatccag
     1081 gagtctgcta gcaaggagca gtattacgtg aggaaagtcc tggagctgca gaccgagttg
     1141 aagcaactca ggaacgttct gaccaacacc caatctgaaa acgagagatt gacaagcgtg
     1201 gcccaagagc tcaaggaaat caatcagaac gtcgagattc aacgcggtag attgagggac
     1261 gacatcaaag aatacaaatt cagggaggca agactcctcc aagactactc agagctggaa
     1321 gaggagaaca tctctctgca gaaacaagtc tcagtgctca ggcaaaatca ggtggagttc
     1381 gaaggactga agcacgaaat caagcgcctc gaagaggaga cagagtacct gaactcccaa
     1441 ctggaggacg ctatcagact gaaggagatt tcagaaagac agctcgaaga agccctggaa
     1501 acactgaaga cagaaaggga gcagaagaat aacctccgca aggagttgag ccactatatg
     1561 tctatcaacg attccttcta cacttcacat ctccaggtca gcttggacgg actgaagttc
     1621 agcgacgaca ctgttaccgc cgagcccaac aatgatgccg aagctctcgt gaacggattc
     1681 gaacatagcg gcctggtcaa atcctcattg gataacaaga ctagcactcc acgcaaggat
     1741 ggtctggctc ctccctctcc ttccttggtg tccgatctgt tgagcgagtt gcacatcagc
     1801 gaaatccaga agctcaagca gcagctcgtc caaatggagc gcgagaaggt gggtctgctg
     1861 gccacattgc aagatactca gaaacagttg gagcaggcta gaggtacctt gtcagagcag
     1921 catgagaagg tgaacaggtt gaccgagaat ctgagcgctc tgaggatgtc taaaggtgaa
     1981 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac
     2041 aaattttctg tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa
     2101 tttatttgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt
     2161 tatggtgttc aatgttttgc gagataccca gatcatatga aacaacatga ctttttcaag
     2221 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac
     2281 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta
     2341 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat
     2401 aactctcaca atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc
     2461 aaaattagac acaacattga agacggttct gttcaattag ctgaccatta tcaacaaaat
     2521 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct
     2581 gccttatcca aggatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact
     2641 gctgctggta ttacctaatg agcttgtcga gaagtactag aggatcataa tcagccatac
     2701 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2761 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2881 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     2941 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     3001 agcaataact atcataaccc ctagggtata cccatctaat tggaaccaga taagtgaaat
     3061 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     3121 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     3181 ccagttccca actattttgt ccgcccacaa ccggtggagg aaattctcct tgaagtttcc
     3241 ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg tccggcgtat
     3301 taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
     3361 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
     3421 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
     3481 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
     3541 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
     3601 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
     3661 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
     3721 ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt gttagcttta
     3781 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
     3841 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
     3901 gtagttgagc tttttggaat taccggttga cttgggtcaa ctgtcagacc aagtttactc
     3961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4741 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattgacttg ggtcgctctt
     4801 cctgtggatg cgcaggtatg tacaggaaga ggtttatact aaactgttac attgcaaacg
     4861 tggtttcgtg tgccaagtgt gaaaaccgat gtttaatcaa ggctctgacg catttctaca
     4921 accacgactc taagtgtgtg ggtgaagtca tgcatgcatc ttttaatcaa atcccaagat
     4981 gtgtataaac caccaaactg ccaaaaaatg aaaactgtcg acaagctctg tccgtttgct
     5041 ggcaactgca agggtctcaa tcctatttgt aattattgaa taataaaaca attataaatg
     5101 tcaaatttgt tttttattaa cgatacaaac caaacgcaac aagaacattt gtagtattat
     5161 ctataattga aaacgcgtag ttataatcgc tgaggtaata tttaaaatca ttttcaaatg
     5221 attcacagtt aatttgcgac aatataattt tattttcaca taaactagac gccttgtcgt
     5281 cttcttcttc gtattccttc tctttttcat ttttctcttc ataaaaatta acatagttat
     5341 tatcgtatcc atatatgtat ctatcgtata gagtaaattt tttgttgtca taaatatata
     5401 tgtctttttt aatggggtgt atagtaccgc tgcgcatagt ttttctgtaa tttacaacag
     5461 tgctattttc tggtagttct tcggagtgtg ttgctttaat tattaaattt atataatcaa
     5521 tgaatttggg atcgtcggtt ttgtacaata tgttgccggc atagtacgca gcttcttcta
     5581 gttcaattac accatttttt agcagcaccg gattaacata actttccaaa atgttgtacg
     5641 aaccgttaaa caaaaacagt tcacctccct tttctatact attgtctgcg agcagttgtt
     5701 tgttgttaaa aataacagcc attgtaatga gacgcacaaa ctaatatcac aaactggaaa
     5761 tgtctatcaa tatatagttg ctgattgcgc agatgccctg cgtaagcggg tgtgggcgga
     5821 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     5881 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     5941 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     6001 ggggcgtggc caagggcatg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     6061 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     6121 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     6181 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     6241 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     6301 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     6361 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     6421 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     6481 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     6541 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     6601 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     6661 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     6721 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     6781 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     6841 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     6901 ttgggcagca gcgaagtcgc cataacttcg tatagcatac attatacgaa gttatctg
//
