LOCUS       sequence-215383-        5071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in 
            Sf9 cells. C-terminal SNAPf-Psc-StrepII tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: Hook3(1-522)-SNAPf-Psc-StrepII
FEATURES             Location/Qualifiers
     source          1..5071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        88..179
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     104..121
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1795..2337
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             2338..2361
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2362..2385
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2422..2445
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2605..2739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2642..2661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2696..2715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2860..3025
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3215..3803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3704..3723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  3938..4161
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4228..4761)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4950..4955,4956..4972,4973..4978))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(join(5049..5071,1..11))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacgaagtta tctgtaacta taacggtcct aaggtagcga gtttaaacac tagtatcgat
       61 tcgcgaccta ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
      121 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
      181 ttccggatta ttcataccgt cccaccatcg ggcgcggatc cctcgagcat gttctctgtt
      241 gaatcattgg agagggcaga attgtgtgag tccctgctga cctggattca gacattcaac
      301 gtggacgctc cctgccagac tgtcgaggat ctgacaaatg gcgtggttat ggctcaggtc
      361 ctccagaaga tcgacccagc atacttcgat gagaactggc tcaacaggat caagaccgaa
      421 gtcggagaca attggaggct caagatttca aacctgaaga agattctcaa gggtatcctc
      481 gactataacc atgaaatctt gggtcaacaa atcaacgatt tcactctccc tgacgtcaat
      541 ctcattggtg agcactctga tgctgctgag ctgggcagga tgctgcaact gatcctggga
      601 tgtgcagtga actgcgagca gaagcaggag tacattcagg caatcatgat gatggaggaa
      661 tccgtgcagc acgtggtgat gactgctatc caggaactca tgtccaagga atctcccgtc
      721 tctgcaggca acgatgctta cgtcgatctc gatcgccagc tgaagaagac aaccgaggaa
      781 ctgaatgagg ctctctctgc aaaggaggag atcgctcagc gctgccacga actggacatg
      841 caggtggcag cattgcagga ggagaagtct agcctgttgg cagagaacca ggtcttgatg
      901 gagaggttga accagtcaga ttctatcgaa gacccaaata gcccagctgg tagaagacac
      961 ctgcaattgc aaactcaact ggagcagctc caggaggaga catttcgcct ggaggcagct
     1021 aaggacgact accgcatccg ctgcgaggaa ttggagaaag aaatcagcga actcaggcaa
     1081 cagaacgatg agctgactac cctcgcagac gaggctcaat ccctgaagga tgagatcgat
     1141 gtgttgaggc acagctcaga taaagtgtcc aagctggaag gtcaggtgga gtcctacaag
     1201 aagaaattgg aggatctcgg tgatctgcgc aggcaggtta aactgctcga agagaagaac
     1261 accatgtata tgcagaatac agtctccctg gaagaggaac tcagaaaggc caatgctgca
     1321 agatcacagt tggaaactta caagagacaa gtcgtcgagt tgcagaatag gctgtctgag
     1381 gaaagcaaga aagctgacaa gctggacttt gaatacaaga gattgaaaga gaaggttgac
     1441 tccctgcaga aggagaaaga taggttgagg actgagaggg actcactgaa agagacaatc
     1501 gaggagctga gatgcgtcca ggctcaagaa ggccagctga ccacacaggg actcatgcct
     1561 ctgggctctc aggagtctag cgattcactg gcagccgaga tcgttactcc agaaatcaga
     1621 gagaagctca ttcgcctgca gcacgagaac aagatgctca aactcaacca agagggttca
     1681 gacaacgaga agatcgcact gttgcagtct ttgctggacg acgcaaactt gaggaagaat
     1741 gaactcgaaa ctgagaacag gctggtgaat cagaggttgc tggaagtgca atcagacaaa
     1801 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
     1861 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
     1921 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
     1981 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
     2041 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
     2101 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
     2161 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
     2221 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
     2281 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtctg
     2341 gaagttctgt ttcagggtcc gtggtcacat ccgcagtttg aaaaaggtag cgcaggtagt
     2401 gcagcaggta gcggtgcagg ttggagccat cctcagtttg agaaataagg tacctctaga
     2461 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     2521 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2581 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2701 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga
     2761 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     2821 ataactatca taacccctag ggtataccca tctaattgga accagataag tgaaatctag
     2881 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc
     2941 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag
     3001 ttcccaacta ttttgtccgc ccacaaccgg ttgacttggg tcaactgtca gaccaagttt
     3061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattga cttgggtcgc
     3901 tcttcctgtg gatgcgcaga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     3961 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     4021 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     4081 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     4141 gggcatgtaa agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
     4201 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
     4261 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
     4321 atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt
     4381 gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat
     4441 atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac
     4501 aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc
     4561 gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc
     4621 gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg
     4681 aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac
     4741 atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     4801 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     4861 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     4921 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     4981 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     5041 aagtcgccat aacttcgtat agcatacatt a
//
