LOCUS       sequence-215384-        5221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in 
            Sf9 cells. C-terminal GFP-Psc-StrepII tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz 
            A, Carter AP
  TITLE     Cryo-EM shows how dynactin recruits two dyneins for faster movement.
  JOURNAL   Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.
  PUBMED    29420470
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-02-7- 7"; volume: "554"; issue: "7691"; pages:
            "202-206"
COMMENT     Sequence Label: Hook3(1-522)-GFP-Psc-StrepII
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2576..2599
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2600..2623
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2660..2683
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    2843..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2880..2899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2934..2953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3098..3263
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3453..4041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3942..3961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4176..4399
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4466..4999)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5188..5193,5194..5210,5211..5216))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcggatccc tcgagcatgt tctctgttga atcattggag agggcagaat tgtgtgagtc
      361 cctgctgacc tggattcaga cattcaacgt ggacgctccc tgccagactg tcgaggatct
      421 gacaaatggc gtggttatgg ctcaggtcct ccagaagatc gacccagcat acttcgatga
      481 gaactggctc aacaggatca agaccgaagt cggagacaat tggaggctca agatttcaaa
      541 cctgaagaag attctcaagg gtatcctcga ctataaccat gaaatcttgg gtcaacaaat
      601 caacgatttc actctccctg acgtcaatct cattggtgag cactctgatg ctgctgagct
      661 gggcaggatg ctgcaactga tcctgggatg tgcagtgaac tgcgagcaga agcaggagta
      721 cattcaggca atcatgatga tggaggaatc cgtgcagcac gtggtgatga ctgctatcca
      781 ggaactcatg tccaaggaat ctcccgtctc tgcaggcaac gatgcttacg tcgatctcga
      841 tcgccagctg aagaagacaa ccgaggaact gaatgaggct ctctctgcaa aggaggagat
      901 cgctcagcgc tgccacgaac tggacatgca ggtggcagca ttgcaggagg agaagtctag
      961 cctgttggca gagaaccagg tcttgatgga gaggttgaac cagtcagatt ctatcgaaga
     1021 cccaaatagc ccagctggta gaagacacct gcaattgcaa actcaactgg agcagctcca
     1081 ggaggagaca tttcgcctgg aggcagctaa ggacgactac cgcatccgct gcgaggaatt
     1141 ggagaaagaa atcagcgaac tcaggcaaca gaacgatgag ctgactaccc tcgcagacga
     1201 ggctcaatcc ctgaaggatg agatcgatgt gttgaggcac agctcagata aagtgtccaa
     1261 gctggaaggt caggtggagt cctacaagaa gaaattggag gatctcggtg atctgcgcag
     1321 gcaggttaaa ctgctcgaag agaagaacac catgtatatg cagaatacag tctccctgga
     1381 agaggaactc agaaaggcca atgctgcaag atcacagttg gaaacttaca agagacaagt
     1441 cgtcgagttg cagaataggc tgtctgagga aagcaagaaa gctgacaagc tggactttga
     1501 atacaagaga ttgaaagaga aggttgactc cctgcagaag gagaaagata ggttgaggac
     1561 tgagagggac tcactgaaag agacaatcga ggagctgaga tgcgtccagg ctcaagaagg
     1621 ccagctgacc acacagggac tcatgcctct gggctctcag gagtctagcg attcactggc
     1681 agccgagatc gttactccag aaatcagaga gaagctcatt cgcctgcagc acgagaacaa
     1741 gatgctcaaa ctcaaccaag agggttcaga caacgagaag atcgcactgt tgcagtcttt
     1801 gctggacgac gcaaacttga ggaagaatga actcgaaact gagaacaggc tggtgaatca
     1861 gaggttgctg gaagtgcaat caggttcttc taaaggtgaa gaattattca ctggtgttgt
     1921 cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga
     1981 aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa
     2041 attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc aatgttttgc
     2101 gagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta
     2161 tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt
     2221 caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga
     2281 agatggtaac attttaggtc acaaattgga atacaactat aactctcaca atgtttacat
     2341 catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac acaacattga
     2401 agacggttct gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc
     2461 agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca aggatccaaa
     2521 cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta ttaccctgga
     2581 agttctgttt cagggtccgt ggtcacatcc gcagtttgaa aaaggtagcg caggtagtgc
     2641 agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag aaataaggta cctctagagc
     2701 ctgcagtctc gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     2761 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2821 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag
     3001 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     3061 aactatcata acccctaggg tatacccatc taattggaac cagataagtg aaatctagtt
     3121 ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca
     3181 tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt
     3241 cccaactatt ttgtccgccc acaaccggtt gacttgggtc aactgtcaga ccaagtttac
     3301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattgact tgggtcgctc
     4141 ttcctgtgga tgcgcagatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa
     4201 ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa
     4261 actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc
     4321 aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg
     4381 gcatgtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     4441 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     4501 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     4561 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     4621 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     4681 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     4741 ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     4801 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     4861 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     4921 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     4981 cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     5041 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     5101 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     5161 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     5221 g
//
