LOCUS       sequence-21540-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Kang Y, He W, Tulley S, Gupta GP, Serganova I, Chen CR, 
            Manova-Todorova K, Blasberg R, Gerald WL, Massague J
  TITLE     Breast cancer bone metastasis mediated by the Smad tumor suppressor 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Sep 27. 102(39):13909-14.
  PUBMED    16172383
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Sep 27. 102(39):13909-14."
COMMENT     Sequence Label: pcDNA Flag-Smad4M
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             167..190
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga
      121 tccagaattc agatctggta ccacgcgtat cgataagctt accatggact acaaggacga
      181 cgatgacaag gacaatatgt ctattacgaa tacaccaaca agtaatgatg cctgtctgag
      241 cattgtgcat agtttgatgt gccatagaca aggtggagag agtgaaacat ttgcaaaaag
      301 agcaattgaa agtttggtaa agaagctgaa ggagaaaaaa gatgaattgg attctttaat
      361 aacagctata actacaaatg gagctcatcc tagtaaatgt gttaccatac agagaacatt
      421 ggatgggagg cttcaggtgg ctggtcggaa aggatttcct catgtgatct atgcccgtct
      481 ctggaggtgg cctgatcttc acaaaaatga actaaaacat gttaaatatt gtcagtatgc
      541 gtttgactta aaatgtgata gtgtctgtgt gaatccatat cactacgaac gagttgtatc
      601 acctggaatt gatctctcag gattaacact gcagagtaat gctccatcaa gtatgatggt
      661 gaaggatgaa tacgttcatg actttgaggg acagccatcg ttgtccactg aaggacat
//
