LOCUS       sequence-21549-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Thomas DA, Massague J
  TITLE     TGF-beta directly targets cytotoxic T cell functions during tumor 
            evasion of immune surveillance.
  JOURNAL   Cancer Cell. 2005 Nov . 8(5):369-80.
  PUBMED    16286245
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2005 Nov . 8(5):369-80."
COMMENT     Sequence Label: pRS-shTGFbeta1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..103)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(88..104)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(122..538)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(138..154)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(170..192)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tctgtatgag acacagatcc ccggagacgg aatacagggc tttcaagaga agccctgtat
       61 tccgtctcct tattgtaaaa gcttatcgat accgtcgacc tcgagagatc gatctaattc
      121 cggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
      181 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
      241 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
      301 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
      361 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
      421 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
      481 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct
      541 aattttcaga caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc
      601 gctgcagcag acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacggag
      661 gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatcctgac ggggtcggat
      721 tccacatcga ctcccttcct caggtcgggc cacaaaaacg gcccccaaag tccctgggac
      781 gtctcccagg gttgcggccg ggtgttcaga actcgtcagt tccaccacgg gtccgccaga
      841 tacagagcta gttagctaac tagtacagac gcaggcgcaa ac
//
