LOCUS       sequence-215506-        3108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone containing tagRFP with a signal peptide and KDEL
            ER retention signal for expression of tagRFP in the ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Nunes-Hasler P, Maschalidi S, Lippens C, Castelbou C, Bouvet S, 
            Guido D, Bermont F, Bassoy EY, Page N, Merkler D, Hugues S, 
            Martinvalet D, Manoury B, Demaurex N
  TITLE     STIM1 promotes migration, phagosomal maturation and antigen 
            cross-presentation in dendritic cells.
  JOURNAL   Nat Commun. 2017 Nov 24;8(1):1852. doi: 10.1038/s41467-017-01600-6.
  PUBMED    29176619
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-24"; volume: "8(1):1852. doi"; pages: " 
            10.1038/s41467-017-01600-6"
COMMENT     Sequence Label: pENTR1a-tagRFP-KDEL
FEATURES             Location/Qualifiers
     source          1..3108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             574..1305
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    complement(1337..1436)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1548..1567)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1559..2368
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1626..1645)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2458..3046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2947..2966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaagccg ctagcccggg atcgccacca tgctgctgcc cgtccccctg
      541 ctgctgggcc tgctgggcgc cgccgccgac atcatggtga gcaagggcga agagctgatt
      601 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
      661 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      721 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
      781 agcagaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct
      841 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      901 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      961 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag
     1021 atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg
     1081 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
     1141 aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc
     1201 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     1261 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagaagga cgagctgtaa
     1321 atctcgagat atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1381 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1441 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1501 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1561 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1621 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1681 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1741 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1801 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1861 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1921 gctggcagtg tccctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1981 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2041 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2101 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2161 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2221 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2281 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2341 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacattattc
     2401 agattgggcc ccgttccact gagcgtcaga cccggtagaa aagatcaaag gatcttcttg
     2461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
