LOCUS       sequence-215510-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORAI1 channel with C-terminal YFP, carrying the mutation V107M in 
            the protein 1st transmembrane domain, responsible for a constitutive
            activity and associated with tubular aggregate myopathy (TAM)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Bulla M, Gyimesi G, Kim JH, Bhardwaj R, Hediger MA, Frieden M, 
            Demaurex N
  TITLE     ORAI1 channel gating and selectivity is differentially altered by 
            natural mutations in the first or third transmembrane domain.
  JOURNAL   J Physiol. 2018 Nov 1. doi: 10.1113/JP277079.
  PUBMED    30382595
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Physiol. 
            2018 Nov 1. doi: 10.1113/JP277079."
COMMENT     Sequence Label: ORAI1-YFP V107M
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1005..1014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1056..1077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagatgc
       61 atccggagcc cgccccgccc ccgagccgta gcagtcccga gcttccccca agcggcggca
      121 gcaccaccag cggcagccgc cggagccgcc gccgcagcgg ggacggggag cccccggggg
      181 ccccgccacc gccgccgtcc gccgtcacct acccggactg gatcggccag agttactccg
      241 aggtgatgag cctcaacgag cactccatgc aggcgctgtc ctggcgcaag ctctacttga
      301 gccgcgccaa gcttaaagcc tccagccgga cctcggctct gctctccggc ttcgccatgg
      361 tggcaatggt ggagatgcag ctggacgctg accacgacta cccaccgggg ctgctcatcg
      421 ccttcagtgc ctgcaccaca gtgctggtgg ctgtgcacct gtttgcgctc atgatcagca
      481 cctgcatcct gcccaacatc gaggcggtga gcaacgtgca caatctcaac tcggtcaagg
      541 agtcccccca tgagcgcatg caccgccaca tcgagctggc ctgggccttc tccaccgtca
      601 ttggcacgct gctcttccta gctgaggtgg tgctgctctg ctgggtcaag ttcttgcccc
      661 tcaagaagca gccaggccag ccaaggccca ccagcaagcc ccccgccagt ggcgcagcag
      721 ccaacgtcag caccagcggc atcaccccgg gccaggcagc tgccatcgcc tcgaccacca
      781 tcatggtgcc cttcggcctg atctttatcg tcttcgccgt ccacttctac cgctcactgg
      841 ttagccataa gaccgaccga cagttccagg agctcaacga gctggcggag tttgcccgct
      901 tacaggacca gctggaccac agaggggacc accccctgac gcccggcagc cactatgccc
      961 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
     1021 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1081 acggccacaa
//
