LOCUS       sequence-215513-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORAI1 channel with C-terminal YFP, carrying the double mutation 
            L273D-L276D in the STIM1 binding domain, leading to a channel gating
            deficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Bulla M, Gyimesi G, Kim JH, Bhardwaj R, Hediger MA, Frieden M, 
            Demaurex N
  TITLE     ORAI1 channel gating and selectivity is differentially altered by 
            natural mutations in the first or third transmembrane domain.
  JOURNAL   J Physiol. 2018 Nov 1. doi: 10.1113/JP277079.
  PUBMED    30382595
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Physiol. 
            2018 Nov 1. doi: 10.1113/JP277079."
COMMENT     Sequence Label: ORAI1-YFP L273D-L276D
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1004..1013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1055..1076)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagatgca
       61 tccggagccc gccccgcccc cgagccgtag cagtcccgag cttcccccaa gcggcggcag
      121 caccaccagc ggcagccgcc ggagccgccg ccgcagcggg gacggggagc ccccgggggc
      181 cccgccaccg ccgccgtccg ccgtcaccta cccggactgg atcggccaga gttactccga
      241 ggtgatgagc ctcaacgagc actccatgca ggcgctgtcc tggcgcaagc tctacttgag
      301 ccgcgccaag cttaaagcct ccagccggac ctcggctctg ctctccggct tcgccatggt
      361 ggcaatggtg gaggtgcagc tggacgctga ccacgactac ccaccggggc tgctcatcgc
      421 cttcagtgcc tgcaccacag tgctggtggc tgtgcacctg tttgcgctca tgatcagcac
      481 ctgcatcctg cccaacatcg aggcggtgag caacgtgcac aatctcaact cggtcaagga
      541 gtccccccat gagcgcatgc accgccacat cgagctggcc tgggccttct ccaccgtcat
      601 tggcacgctg ctcttcctag ctgaggtggt gctgctctgc tgggtcaagt tcttgcccct
      661 caagaagcag ccaggccagc caaggcccac cagcaagccc cccgccagtg gcgcagcagc
      721 caacgtcagc accagcggca tcaccccggg ccaggcagct gccatcgcct cgaccaccat
      781 catggtgccc ttcggcctga tctttatcgt cttcgccgtc cacttctacc gctcactggt
      841 tagccataag accgaccgac agttccagga ggacaacgag gacgcggagt ttgcccgctt
      901 acaggaccag ctggaccaca gaggggacca ccccctgacg cccggcagcc actatgcccg
      961 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
     1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1081 cggccacaa
//
