LOCUS       sequence-215535-        9823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with an 
            N-terminal Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Taipale M, Krykbaeva I, Koeva M, Kayatekin C, Westover KD, Karras 
            GI, Lindquist S
  TITLE     Quantitative analysis of HSP90-client interactions reveals 
            principles of substrate recognition.
  JOURNAL   Cell. 2012 Aug 31;150(5):987-1001. doi: 10.1016/j.cell.2012.06.047.
  PUBMED    22939624
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06"; journalName: "Cell"; date: "2012-08-31- 
            31"; volume: "150"; issue: "5"; pages: "987-1001"
COMMENT     Sequence Label: pLenti6.2-Renilla-ccdB
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1653..1956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1957..2160
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2111..2131
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2157..2181
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2247..2256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2253..3185
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    3231..3355
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3380..3385,3386..3403,3404..3410)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3464..4123
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3530..3549)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4465..4770
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4649..4668
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4811..4935)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4977..5018
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        5051..5550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5086..5109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5483..5502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5564..5611
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5630..6028
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6115..6348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6420..6541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6457..6476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6511..6530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6581..6716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6736..6755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6737..6755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6765..6782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6765..6781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6774..6796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6923..7378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7010..7029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7220..7241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7404..7508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7509..7577,7578..8369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7727..7746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8540..9128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9029..9048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9282..9299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9416..9437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9452..9457,9458..9475,9476..9482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9490..9506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9495..9517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9514..9530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9514..9530
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9549..9569
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9551..9569
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9597..9823
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat gtcgacgtta acgctagcga ctgggtctgc gatgtcgacg ttaacgctag
     1621 cgactgggtc tgcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1681 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1741 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1801 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1861 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1921 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1981 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2041 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2101 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2161 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     2221 agacaccgac tctagaggat ccagccgcca ccatggcttc caaggtgtac gaccccgagc
     2281 aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc
     2341 tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc
     2401 tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg
     2461 tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg
     2521 gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc
     2581 ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact
     2641 cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga
     2701 tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag
     2761 agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga
     2821 tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg
     2881 gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca
     2941 agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc
     3001 tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag
     3061 ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg
     3121 acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg
     3181 agcagagtgg cggccgctcg agtggctcgg gctcgacctc gggctcgggc acaagtttgt
     3241 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     3301 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     3361 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     3421 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     3481 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     3541 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     3601 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     3661 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     3721 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     3781 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     3841 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     3901 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     3961 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     4021 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     4081 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     4141 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     4201 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     4261 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     4321 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     4381 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     4441 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     4501 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     4561 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     4621 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     4681 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     4741 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     4801 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     4861 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     4921 cttgtacaaa gtggttgctc tatagtaact cgagtctaga gggcccgcgg ttcgaaggta
     4981 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgacgat
     5041 ggtacctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5101 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5161 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5221 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5281 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5341 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5401 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     5461 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     5521 ttctcctctt cctcatctcc gggcctttcg actctagaca cgtgttgaca attaatcatc
     5581 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc
     5641 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     5701 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     5761 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     5821 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     5881 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5941 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     6001 gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa
     6061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6121 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6361 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     6421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6541 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     6601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6721 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6901 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7321 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     7381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9241 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9301 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9361 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9421 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9481 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9541 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     9601 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     9661 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     9721 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     9781 ttgcagagat attgtattta agtgcctagc tcgatacata aac
//
