LOCUS       sequence-215536-       15820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prp8-TAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Umen JG, Guthrie C
  TITLE     Mutagenesis of the yeast gene PRP8 reveals domains governing the 
            specificity and fidelity of 3' splice site selection.
  JOURNAL   Genetics. 1996 Jun;143(2):723-39.
  PUBMED    8725222
REFERENCE   2  (bases 1 to 15820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1996-06"; volume: "143(2)"; pages: "723-39"
COMMENT     Sequence Label: pJU225 Prp8-TAP
FEATURES             Location/Qualifiers
     source          1..15820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..40
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     32..49
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     33..49
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             877..894
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8061..8138
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             8166..8186
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      8630..8817
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            8892..10248
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        8892..9235
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             9236..10045
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLARAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9303..9322)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      10051..10248
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(10405..10425)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10405..10423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10444..10460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10444..10460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10457..10479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10468..10484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10492..10498,10499..10516,10517..10522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10537..10558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10675..10692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10846..11434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10926..10945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11605..12396,12397..12465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12228..12247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12466..12570)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12597..13939
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(13560..13607)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13980..13998
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14036..14058)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14158..14177
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        14198..14479
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             14480..15154
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(15257..15712)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15394..15415)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15606..15625
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
       61 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgagactt caaagcatgg
      121 tttggtttgg gccaggctta tgctctcctg gacatgcatt tatattctct ttactacttc
      181 cagaaagctt gcactttgaa accttgggat cgtcggattt ggcaagtatt gggagaatgt
      241 tatagtaaga cgggaaataa ggtagaagct ataaaatgct acaaaagatc cataaaagct
      301 tcacaaacgg tcgatcaaaa tacttcaata tattaccggt tagcgcaact atatgaagaa
      361 cttgaagact tgcaagaatg taagaagttc atgatgaaat gtgtagatgt ggaagaactt
      421 ctggaaggta tagtaacaga tgaaaccgtg aaggctaggc tttggctggc aatatttgag
      481 attaaggcag gaaactacca attggcttat gattatgcca tgggggtatc tagtggaacg
      541 tctcaagaga ttgaagaggc tcgtatgctg gctcgggagt gcagaaggca tatgtagtga
      601 agtgaacata cacatagcta ttcgtactaa atgatatgaa atttttataa atgccaggct
      661 atatagctat ttaaagtgac catggcagaa ggatgaaccg aggtaatacg gctagtacaa
      721 aagcaacaaa gttaggaata caatttgaga aacgaagacc atagaaaata cttgtgcgat
      781 tgaacttcct tccaaaaaaa aaatagcgtc aaagaaagat gagtggacta ccgcccccac
      841 ctcctggttt tgaagaggac agcgacttag cacttccacc accaccacca ccaccgcctg
      901 gatacgaaat cgaagaactg gataatccga tggtgccatc atcggtaaat gaggatacat
      961 tccttccgcc tccaccacct cctccaagca acttcgaaat aaacgctgaa gaaattgtgg
     1021 acttcacatt accaccgcca ccaccccctc caggtctaga tgaattagag actaaagcag
     1081 aaaagaaagt agaattacat ggaaagagaa aattggacat aggaaaagat actttcgtca
     1141 ctcgaaaatc aagaaagcgt gcaaaaaaaa tgaccaaaaa ggcaaagaga agcaatttat
     1201 atactcctaa agccgagatg ccaccggaac atctgcgaaa gattattaat actcacagtg
     1261 acatggcctc caaaatgtac aatactgata aaaaggcttt tctaggcgct ctaaagtatt
     1321 taccgcacgc gattttaaaa ttgttagaga acatgcctca tccatgggag caagccaaag
     1381 aagttaaagt tttatatcac acttcaggag ccataacatt tgtgaatgaa acaccaagag
     1441 taatagagcc tgtatatact gcccaatggt cagctacttg gatagcaatg aggagagaaa
     1501 aacgagatag aacacatttt aaaagaatga gatttccacc tttcgatgat gacgaaccac
     1561 ctctgtcata tgaacagcac atagaaaata tcgagccact ggatcctata aatcttccct
     1621 tagactctca agatgatgaa tatgttaaag attggctcta cgattcccgg cccttggagg
     1681 aggatagtaa aaaggtcaat ggtacatctt ataaaaagtg gagttttgat ttacctgaaa
     1741 tgtcgaactt atataggtta tcaacgccac ttagagatga agtaactgac aaaaattact
     1801 attatctttt cgataaaaaa tcttttttta acggtaaagc ccttaataac gcaatccctg
     1861 gaggtcctaa atttgaacct ttatacccaa gagaagagga ggaagattat aacgaattca
     1921 actctataga tagagtaata tttagggttc ctattagaag cgaatataag gtagcgtttc
     1981 ctcatctata taattcccgg ccccgttcag tgcgcatacc atggtataat aatccagtgt
     2041 cgtgtattat ccagaacgat gaggagtacg acacgcctgc actttttttt gatccatcgc
     2101 tgaacccaat acctcacttt attgataata attcatccct caacgtatca aatacaaagg
     2161 aaaacggtga tttcacttta cctgaagatt ttgcacctct tttggctgaa gaagaagaac
     2221 ttattttgcc aaatacaaaa gatgcgatgt cgttatatca ttcacctttt ccatttaata
     2281 gaactaaagg aaaaatggtg agagcacaag atgttgccct ggctaaaaaa tggtttttgc
     2341 aacatcctga cgaggagtat cccgttaaag ttaaggtctc ttaccaaaag ctcttgaaga
     2401 actacgtgct aaatgaattg caccctactt tgccaacaaa ccacaacaaa actaagttat
     2461 tgaagagttt aaaaaatacc aagtattttc aacaaactac aattgattgg gtagaggcag
     2521 gactccagct atgtcgacaa ggacacaata tgttaaattt attaatccac agaaagggcc
     2581 taacttattt acatctcgac tacaatttca atttaaaacc aactaaaacc ttaaccacaa
     2641 aagaacgtaa aaagtcaaga cttggaaact catttcattt gatgcgtgaa ttgctaaaaa
     2701 tgatgaaact gattgtagat acacatgtgc agtttaggtt aggaaatgta gatgccttcc
     2761 agttagcaga tggtatacat tatattttga accacatagg acaattgacc ggtatttatc
     2821 gttataaata taaagtaatg catcaaatcc gcgcatgtaa agatctgaaa catatcatat
     2881 attataagtt taataaaaat ttgggaaaag gacctggttg tggattttgg caacctgcgt
     2941 ggagagtgtg gctaaatttc ttaagaggca ctataccact actcgaaaga tatattggaa
     3001 accttatcac acgtcaattt gaaggccgtt ctaatgaaat tgtcaaaact acaactaaac
     3061 aaagacttga cgcctactac gatcttgagt tacgaaactc ggtcatggat gacattttag
     3121 aaatgatgcc tgagagtatt cgacagaaaa aggcaagaac tatactgcaa catttaagtg
     3181 aggcttggag atgttggaag gctaatattc catgggatgt tcctggtatg ccagccccaa
     3241 tcaaaaaaat aattgaacgg tatataaaat ccaaagctga tgcctgggta tctgcagctc
     3301 attacaatcg agaacgtata aagcgtggag ctcacgtcga aaagactatg gttaaaaaaa
     3361 atctggggcg tttaacgaga ctttggatca aaaatgagca ggaacgtcaa aggcaaatac
     3421 agaaaaacgg tcccgaaatt actcccgaag aggctacgac catattttct gttatggttg
     3481 agtggcttga atcaaggagc ttttcaccaa taccatttcc acctttaact tacaaaaatg
     3541 atactaagat attagttctt gccctcgaag acctaaaaga tgtctacgca tcaaaagtac
     3601 gtttaaatgc atctgaaagg gaagaacttg cgttgataga ggaggcttat gataatcctc
     3661 acgatacttt gaacagaatc aaaaagtact tgttgaccca gcgagttttt aagcctgttg
     3721 atataaccat gatggaaaac tatcaaaaca tttcccctgt ttattcagtt gatcccttgg
     3781 aaaagattac cgatgcatat cttgatcagt atttatggta cgaagctgac caacgaaagc
     3841 ttttcccaaa ctggattaaa ccaagtgatt cagagatacc acctcttctg gtatacaagt
     3901 ggactcaggg tataaacaac ctatctgaaa tttgggatgt atccagaggc caatctgcag
     3961 ttttgcttga gactactttg ggtgaaatgg ccgaaaaaat tgactttact ttacttaata
     4021 gattacttcg cctgattgta gatcctaata ttgctgacta tatcactgca aaaaataatg
     4081 ttgttatcaa ctttaaagat atgagtcacg tcaacaaata tggcttaata cgcgggttga
     4141 agttcgcttc tttcatattc caatattacg gactagttat agatctttta ctattgggtc
     4201 aggaaagggc tacagatttg gctggtccag ctaacaatcc aaatgaattt atgcaattca
     4261 agagcaaaga agtagaaaag gcacatccga tcagacttta caccagatat ttagatcgta
     4321 tatatatgct ttttcacttt gaagaagatg agggagagga attgactgat gaatacttgg
     4381 cagagaatcc agatccaaac tttgaaaaca gcatcggata caataataga aaatgctggc
     4441 ccaaagattc gcgcatgagg ctaatacgtc aggacgttaa cctagggcga gcagtgtttt
     4501 gggaaattca aagtagggtg ccaacttcgt taacgtcaat taaatgggaa aatgcttttg
     4561 tttccgtata cagtaaaaac aatccgaact tactcttctc tatgtgtggt tttgaagtca
     4621 gaatattacc aagacaaaga atggaagagg tggtttctaa cgatgaaggt gtttgggatt
     4681 tagttgacga aagaaccaag caaagaactg caaaagcata tttaaaggta tctgaagaag
     4741 aaatcaaaaa atttgacagc agaataagag gcatattgat ggcctccggt tcaaccactt
     4801 ttactaaagt ggccgctaaa tggaacactt cactgatatc ccttttcaca tactttagag
     4861 aagccattgt tgccacggag ccgttattag atatcctggt gaaaggagaa acaagaattc
     4921 aaaaccgtgt caaacttggt ttaaattcga aaatgccaac aagatttcca cctgctgttt
     4981 tttatacacc aaaagaactt ggtgggttgg gtatgattag tgcttctcat attttaattc
     5041 ctgcatctga cttaagttgg tcaaaacaga ccgatactgg aattacgcat tttcgtgctg
     5101 gtatgacgca cgaggatgaa aaattaatcc caactatctt ccgttacatc actacttggg
     5161 aaaatgaatt tttagactct caaagggtgt gggccgagta tgcaacgaaa aggcaggaag
     5221 ctattcagca gaataggagg ttagcttttg aagaacttga aggatcgtgg gatcgtggta
     5281 tacctcgtat tagcacttta tttcaaagag atcgacatac tctagcgtat gacagaggtc
     5341 acagaatccg tagggaattt aaacagtact ctctagagag aaacagcccc ttttggtgga
     5401 caaactccca ccatgacggt aaattatgga atttaaatgc ctatagaact gatgtgattc
     5461 aagccctagg aggtattgaa acaattttgg aacacacttt atttaagggg actggtttta
     5521 attcatggga aggtttgttt tgggaaaagg cgtccgggtt tgaggattcc atgcaattta
     5581 agaagctaac tcatgctcaa agaactggtt taagccaaat tccaaatcgt agatttacct
     5641 tatggtggtc accaactatt aacagagcaa atgtctatgt cggtttcttg gtgcaattgg
     5701 atctgactgg tatcttcctc catggtaaaa ttcctacgtt aaaaatatct ttaattcaaa
     5761 tattccgtgc tcatttgtgg caaaaaatac atgaaagtat agtttttgat atttgtcaaa
     5821 ttctcgatgg tgagctagat gttttgcaaa ttgaatctgt gactaaagaa acagtgcatc
     5881 cccgcaaatc gtacaagatg aattcatctg ccgctgacat aactatggaa agtgttcatg
     5941 aatgggaagt ttcaaaaccc tccctattac atgaaacaaa tgactcattt aagggattga
     6001 ttaccaacaa aatgtggttt gatgtacaat tgagatacgg tgattatgat tctcatgata
     6061 tatctcgtta tgtgagggca aaattcctcg attatacaac agataatgtc agcatgtatc
     6121 cctccccaac aggtgttatg atcggtatcg atttggctta taacatgtat gatgcctatg
     6181 gtaattggtt caacggatta aaacctttga tacaaaatag tatgagaaca attatgaaag
     6241 ctaacccagc attatatgtc cttcgtgaac gtattagaaa aggtcttcaa atatatcaat
     6301 ctagtgtaca ggaaccattt ttaaactcct caaactatgc cgagttattc aataacgaca
     6361 ttaaactctt cgttgacgac actaatgtgt atagagttac tgtccacaag acttttgaag
     6421 ggaacgttgc tacaaaagca attaatggtt gcattttcac tttaaatcca aagactgggc
     6481 atctgtttct gaaaattatc catacctctg tatgggccgg tcaaaaacgt ttaagccaat
     6541 tggccaaatg gaaaacagct gaggaagtta gtgctcttgt gagatctttg cccaaagaag
     6601 agcagccaaa acaaattatc gtcacaagaa aggcaatgct tgatccttta gaggttcaca
     6661 tgcttgactt tcctaacata gcaatcaggc caacagagct gcgactacca ttttcagctg
     6721 caatgtcaat agataaactt tctgatgttg tgatgaaagc tactgaacct cagatggttc
     6781 tgtttaacat ttatgatgat tggttagacc gtatttcatc gtacactgca ttttctagac
     6841 ttacactact tttaagagct ttaaagacga acgaggaaag tgccaaaatg attctactca
     6901 gtgaccccac gatcactatc aaatcgtacc atttatggcc ttcattcact gatgaacaat
     6961 ggatcactat cgaatctcaa atgagagatt tgattttgac tgagtacggc agaaaataca
     7021 atgtcaatat atctgcctta actcaaacgg aaattaaaga tattatattg ggtcaaaata
     7081 tcaaggcacc atctgtgaag agacaaaaaa tggctgaatt agaagctgcc aggtcagaaa
     7141 agcagaacga cgaagaagct gcaggtgcat caactgttat gaaaactaaa actatcaatg
     7201 cgcaaggtga ggagatagtt gtagtagctt ccgctgatta cgaaagtcaa acgtttagtt
     7261 caaagaatga atggagaaaa tctgcaattg ctaatacact attatatttg aggttaaaaa
     7321 acatttatgt ttcggctgat gatttcgtgg aggaacagaa tgtttacgtc cttccaaaaa
     7381 atttattaaa gaagtttatt gagatatccg atgtgaaaat ccaggtggcg gcctttattt
     7441 atggtatgtc agcaaaagat catccgaagg tgaaagaaat caagactgtt gtgctggtac
     7501 ctcaattagg ccacgtagga tccgttcaga taagcaacat ccctgacatc ggagaccttc
     7561 cggatactga aggccttgaa ctactcggtt ggatccatac ccagacagaa gaacttaaat
     7621 tcatggctgc gtctgaagta gcaactcatt ctaaactttt tgcggataag aaaagagact
     7681 gcattgatat ttcaattttt tccaccccgg gatcagtatc attaagcgct tataacctga
     7741 ccgacgaagg ttaccaatgg ggtgaagaaa ataaggatat aatgaacgtg ctatcggaag
     7801 ggtttgagcc tactttcagc acgcatgccc aattacttct ctctgaccgt atcacaggta
     7861 attttataat tccatctgga aacgtttgga actacacatt tatgggaaca gcatttaacc
     7921 aagaaggtga ctacaatttt aaatacggca ttcctttgga attctataat gagatgcatc
     7981 gtccagtaca ctttttacag tttagcgaat tggcggggga cgaagagtta gaggccgaac
     8041 aaatcgatgt atttagcatg aagcgacgat ggaaaaagaa tttcatagcc gtctcagcag
     8101 ccaaccgctt taagaaaatc tcatcctccg gggcacttga ttatggtacc ccaactactg
     8161 cttctgaaaa tctatatttt caaggtgaac taaaaactgc tgctttggct caacatgcga
     8221 ttaaagctga tgcgcaacaa aataacttca acaaagatca acaaagcgcc ttctatgaaa
     8281 tcttgaacat gcctaactta aacgaagcgc aacgtaacgg cttcattcaa agtcttaaag
     8341 acgacccaag ccaaagcact aacgttttag gtgaagctaa aaaattaaac gaatctcaag
     8401 caccgaaagc tgataacaat ttcaacaaag aacaacaaaa tgctttctat gaaatcttga
     8461 atatgcctaa cttaaacgaa gaacaacgca atggtttcat ccaaagctta aaagatgacc
     8521 caagccaaag tgctaaccta ttgtcggaag ctaaaaagtt aaatgaatct caagcaccga
     8581 aagcggataa caaattcaac aaagaataag gcgcgccact tctaaataag cgaatttctt
     8641 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     8701 aagtgaccct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     8761 caggttgctt tctcaggtat agtatgaggt cgctctcatt gaccacacct ctaccggcag
     8821 atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc ccgccgggtc
     8881 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
     8941 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
     9001 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
     9061 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
     9121 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
     9181 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggg
     9241 taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata
     9301 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     9361 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     9421 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     9481 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa
     9541 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     9601 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     9661 tcgcgtattt cgtctcgctc gggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     9721 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     9781 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     9841 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     9901 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     9961 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
    10021 tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct tgttttcaag
    10081 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
    10141 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
    10201 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
    10261 tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccgcta
    10321 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtagc ggccgccacc
    10381 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
    10441 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10501 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10561 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10621 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10681 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10741 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    11041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    11101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    12001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    12061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg
    12601 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa
    12661 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc
    12721 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt
    12781 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt
    12841 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta
    12901 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt
    12961 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc
    13021 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga
    13081 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg
    13141 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa
    13201 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt
    13261 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt
    13321 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg
    13381 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact
    13441 tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt
    13501 gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg
    13561 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
    13621 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
    13681 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
    13741 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
    13801 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
    13861 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
    13921 tttcctttga tattggatca tactaagaaa ccattattat catgacatta acctataaaa
    13981 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    14041 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    14101 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    14161 catcagagca gattgtactg agagtgcacc atagatcaac gacattacta tatatataat
    14221 ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt tatgacgcca
    14281 gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta cgtacaatct
    14341 tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg
    14401 agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca
    14461 cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt
    14521 gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga
    14581 cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag
    14641 gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt
    14701 tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat
    14761 tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt
    14821 gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc
    14881 acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact
    14941 tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca
    15001 ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg
    15061 cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa
    15121 aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa
    15181 gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag
    15241 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
    15301 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
    15361 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
    15421 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
    15481 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
    15541 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
    15601 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
    15661 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
    15721 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    15781 agctggcgaa agggggatgt gctgcaaggc gattaagttg
//
