LOCUS       sequence-215537-        9844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with a 
            C-terminal Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Taipale M, Krykbaeva I, Koeva M, Kayatekin C, Westover KD, Karras 
            GI, Lindquist S
  TITLE     Quantitative analysis of HSP90-client interactions reveals 
            principles of substrate recognition.
  JOURNAL   Cell. 2012 Aug 31;150(5):987-1001. doi: 10.1016/j.cell.2012.06.047.
  PUBMED    22939624
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06"; journalName: "Cell"; date: "2012-08-31- 
            31"; volume: "150"; issue: "5"; pages: "987-1001"
COMMENT     Sequence Label: pLenti6.2-ccdB-Renilla
FEATURES             Location/Qualifiers
     source          1..9844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1653..1956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1957..2160
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2111..2131
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2157..2181
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2277..2401
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2426..2431,2432..2449,2450..2456)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2510..3169
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2576..2595)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3511..3816
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3695..3714
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3857..3981)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4049..4981
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             4998..5039
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        5072..5571
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5107..5130)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5504..5523
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5585..5632
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5651..6049
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6136..6369
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6441..6562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6478..6497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6532..6551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6602..6737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6757..6776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6758..6776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6786..6803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6786..6802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6795..6817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6944..7399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7031..7050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7241..7262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7425..7529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7530..7598,7599..8390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7748..7767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8561..9149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9050..9069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9303..9320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9437..9458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9473..9478,9479..9496,9497..9503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9511..9527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9516..9538
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9535..9551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9535..9551
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9570..9590
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9572..9590
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9618..9844
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat gtcgacgtta acgctagcga ctgggtctgc gatgtcgacg ttaacgctag
     1621 cgactgggtc tgcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1681 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1741 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1801 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1861 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1921 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1981 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2041 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2101 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2161 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     2221 agacaccgac tctagaggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa
     2281 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2341 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     2401 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     2461 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     2521 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2581 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     2641 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     2701 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     2761 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     2821 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     2881 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     2941 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     3001 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     3061 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     3121 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc
     3181 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     3241 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     3301 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     3361 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     3421 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     3481 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     3541 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     3601 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     3661 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     3721 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     3781 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     3841 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     3901 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     3961 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtggctcggg
     4021 ctcgacctcg ggctcgggca aaaccggtgc ttccaaggtg tacgaccccg agcaacgcaa
     4081 acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc
     4141 cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg
     4201 taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag
     4261 atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata
     4321 tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa
     4381 gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga
     4441 gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc
     4501 ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga
     4561 gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg
     4621 gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt
     4681 tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga
     4741 cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa
     4801 gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa
     4861 gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc
     4921 agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgagcagta
     4981 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     5041 gtaccggtta gtaatgacga tggtacctac cgggtagggg aggcgctttt cccaaggcag
     5101 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     5161 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     5221 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     5281 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     5341 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     5401 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     5461 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     5521 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gactctagac
     5581 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     5641 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     5701 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     5761 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     5821 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     5881 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     5941 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     6001 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga
     6061 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6121 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6361 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6421 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6541 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6601 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6661 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6721 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6781 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6961 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7021 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7381 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8701 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9301 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9541 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9601 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9661 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9721 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9781 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9841 aaac
//
