LOCUS       sequence-215540-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with a 
            C-terminal Nanoluc luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.2-ccdB-Nanoluc
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1653..1956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1957..2160
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2111..2131
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2157..2181
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2305..2429
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2454..2459,2460..2477,2478..2484)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2538..3197
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2604..2623)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3539..3844
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3723..3742
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3885..4009)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4077..4589
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             4606..4647
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4680..5179
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4715..4738)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5112..5131
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5193..5240
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5259..5657
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5744..5977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6049..6170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6086..6105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6140..6159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6210..6345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6365..6384)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6366..6384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6394..6411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6394..6410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6403..6425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6552..7007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6639..6658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6849..6870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7033..7137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7138..7206,7207..7998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7356..7375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8169..8757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8658..8677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8911..8928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9045..9066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9081..9086,9087..9104,9105..9111)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9119..9135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9124..9146
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9143..9159
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9143..9159
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9178..9198
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9180..9198
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9226..9452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat gtcgacgtta acgctagcga ctgggtctgc gatgtcgacg ttaacgctag
     1621 cgactgggtc tgcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1681 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1741 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1801 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1861 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1921 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1981 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2041 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2101 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2161 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     2221 agacaccgac tctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag
     2281 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     2341 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     2401 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     2461 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     2521 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     2581 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     2641 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2701 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     2761 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     2821 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     2881 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     2941 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     3001 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     3061 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     3121 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3181 ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa cagtatgcgt
     3241 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     3301 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     3361 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     3421 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     3481 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     3541 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     3601 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     3661 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     3721 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     3781 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     3841 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     3901 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     3961 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt gatatccagc
     4021 acagtggcgg ccgctcgagt ggctcgggct cgacctcggg ctcgggcaaa accggtgtct
     4081 tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag
     4141 tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga
     4201 tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc
     4261 cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt
     4321 accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg
     4381 gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg
     4441 acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc
     4501 gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct
     4561 ggcggctgtg cgaacgcatt ctggcgtaag ggcccgcggt tcgaaggtaa gcctatccct
     4621 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgacgatg gtacctaccg
     4681 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4741 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     4801 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     4861 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     4921 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     4981 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5041 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5101 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5161 ctcatctccg ggcctttcga ctctagacac gtgttgacaa ttaatcatcg gcatagtata
     5221 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag
     5281 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     5341 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     5401 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     5461 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     5521 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     5581 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     5641 atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag
     5701 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5761 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5821 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5881 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5941 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6001 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6181 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6361 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6421 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6481 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6541 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6901 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6961 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7021 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7081 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7141 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7201 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7261 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7321 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7381 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7441 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7501 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7561 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7621 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7681 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7741 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7801 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7861 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7921 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7981 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8041 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8881 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8941 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9001 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9061 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9121 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     9181 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     9241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     9301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     9361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     9421 ttgtatttaa gtgcctagct cgatacataa ac
//
