LOCUS       sequence-215543-        9687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses miR-181 c and d.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  TITLE     van Oers mIR plasmids
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGC-U6-miR-181cd
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           63..156
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           846..958
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1028..2239
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2076..2095
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2287..2303)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2288..2304)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(2306..2308,2309..2311,2312..3025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2351..2372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2612..2631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2959..2980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3294..3763
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4463..4480)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4463..4479)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4472..4494)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4688..4707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4807..4829
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4867..4885)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4953..5057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5058..5126,5127..5918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5276..5295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6089..6677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6578..6597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6831..6848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6965..6986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7001..7006,7007..7024,7025..7031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7039..7055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7044..7066
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7063..7079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7063..7079
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7605..8074
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8139..8496
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8569..8985
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8915..8937
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8953..8969
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     9299..9315
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        9345..9363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9447..9687,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9447..9467
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9618..9637
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctagggataa cagggtaatt
       61 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      121 tgggattact tcttcaggtt aacccaacag aaggctcgat aattgaattc tgagtctcca
      181 aaaaagaata actgatggct gattttgtga gtcaaagggg accctggttt ctctctcgtc
      241 ccacatgctc tctgccttgc tggcctactc tcccaactcc agttatccaa gaacttgcca
      301 agggtttggg ggaacattca acctgtcggt gagtttgggc agctcagaca aaccatcgac
      361 cgttgagtgg accccgaggc ctggaactgc cacccgtcta ccccatcccc accctgtaga
      421 cccgggagag ccccaggcag catcccctgc ctcaggccac agcaaaggtc acaattaaca
      481 ttcattgttg tcggtgggtt gtgaggaggc agccagaccc accgggggat gaatgtcact
      541 gtggctgggc caaacaacac catcagggat gggaaagagg atagaaccct tcagattttg
      601 cttgtcactt actccccttg gggaaatggg actggtgtct gggatcttga tgggctgatt
      661 ggaattggga aataaaccct ggagggttag gtgttgggga gatcctaaat tgggagactc
      721 tggggggatg atgtttgctc agtgatgctc tgcagtgggc aaactggtag atgaacatgt
      781 gacagataca tgggcaggtg gatggatgag cgggaatgaa gtctgtccta gcagtggtga
      841 ctgaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc
      901 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
      961 tcaattggaa gactaatgcg gcctcgacat tgattattga ctagtaaccc gtgtcggctc
     1021 cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     1081 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
     1141 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
     1201 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
     1261 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
     1321 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
     1381 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
     1441 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
     1501 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
     1561 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
     1621 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
     1681 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
     1741 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     1801 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     1861 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
     1921 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     1981 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     2041 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     2101 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     2161 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     2221 tttggctttt ttgttagacg aagcttgggc tgcaggtcga gctcaagctt cggatcctgc
     2281 agtcgacgat accgtcgacc tcgacatggt gagcaagggc gaggagctgt tcaccggggt
     2341 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2401 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2461 caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt
     2521 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2581 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2641 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2701 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2761 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2821 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2881 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2941 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3001 cggcatggac gagctgtaca agtaaggatc cggattagtc caatttgtta aagacaggat
     3061 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     3121 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     3181 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     3241 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     3301 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     3361 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     3421 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     3481 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     3541 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     3601 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     3661 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     3721 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     3781 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagtac ttaaagttac
     3841 attggcttcc ttgaaataaa catggagtat tcagaatgtg tcataaatat ttctaatttt
     3901 aagatagtat ctccattggc tttctacttt ttcttttatt tttttttgtc ctctgtcttc
     3961 catttgttgt tgttgttgtt tgtttgtttg tttgttggtt ggttggttaa ttttttttta
     4021 aagatcctac actatagttc aagctagact attagctact ctgtaaccca gggtgacctt
     4081 gaagtcatgg gtagcctgct gttttagcct tcccacatct aagattacag gtatgagcta
     4141 tcatttttgg tatattgatt gattgattga ttgatgtgtg tgtgtgtgat tgtgtttgtg
     4201 tgtgtgattg tgtatatgtg tgtatggttg tgtgtgattg tgtgtatgta tgtttgtgtg
     4261 tgattgtgtg tgtgtgattg tgcatgtgtg tgtgtgtgat tgtgtttatg tgtatgattg
     4321 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgttgtgt atatatattt
     4381 atggtagtga gaggcaacgc tccggctcag gtgtcaggtt ggtttttgag acagagtctt
     4441 tcacttagct tggaattaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     4621 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     4681 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4741 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4801 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4861 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4921 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6961 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7021 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7081 atgattacgc caagctttgc tcttaggagt ttcctaatac atcccaaact caaatatata
     7141 aagcatttga cttgttctat gccctagggg gcggggggaa gctaagccag ctttttttaa
     7201 catttaaaat gttaattcca ttttaaatgc acagatgttt ttatttcata agggtttcaa
     7261 tgtgcatgaa tgctgcaata ttcctgttac caaagctagt ataaataaaa atagataaac
     7321 gtggaaatta cttagagttt ctgtcattaa cgtttccttc ctcagttgac aacataaatg
     7381 cgctgctgag aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt
     7441 aattactagt caattagttg atttttattt ttgacatata catgtgaaag accccacctg
     7501 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     7561 gagaatagaa aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     7621 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     7681 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     7741 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     7801 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     7861 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     7921 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     7981 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     8041 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
     8101 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg
     8161 tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta
     8221 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc
     8281 cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc
     8341 caaaaatccc gatcgttttg gactctttgg tgcacccccc ttagaggagg gatatgtggt
     8401 tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt
     8461 ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg
     8521 tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ccgcggcggg ccagactgtt
     8581 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     8641 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     8701 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     8761 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     8821 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     8881 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     8941 cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat atgagatctt
     9001 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
     9061 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
     9121 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
     9181 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
     9241 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
     9301 ttggatacac gccgcccacg taaaacaatt gaagatctag tcgaaattaa ccctcactaa
     9361 agggaacaaa agctggagct ccaccgcggt ggcggccgtg acggagcgtg accgcgcgcc
     9421 gagcgcgcgc cagggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc
     9481 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     9541 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     9601 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     9661 tggctttata tatcttgtgg aaaggac
//
