LOCUS       sequence-215544-       12336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used 
            with suicide plasmid for broad-host-range genome alteration).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK601
FEATURES             Location/Qualifiers
     source          1..12336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(551..569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             830..4936
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRHKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4957..5088
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   5089..5124
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     terminator      5140..5188
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(5401..6192)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(6572..7423)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(7410..8249)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8760..9731)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10970..11057
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      11399..11793
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 acgtaaatca ggtgaaactg accgataata cgactcacta taggtatgaa ctaatttagt
       61 accctatcca agaattcatt tcccattggt tacctatgcg ccagtcattt ccgctgaaaa
      121 agcgtatcac gaacaacttt ctgtagcgga aatcacgaat gcttgcttcg aaccggctaa
      181 tcagatggta aaatgcgatc ctcgtcatgg aaaatatatg gcgtgttgta tgctgtacag
      241 aggagatgtt gtacctaagg atgttaatgc agccattgca actatcaaga ctaagcgcac
      301 cattcaattt gtcgattggt gcccaactgg attcaaagtt ggcattaatt atcaaccacc
      361 tactgttgta ccaggtggtg atcttgctaa agtacaacga gctgtttgca tgttatccaa
      421 tactactgct atcgcagaag cttgggccag acttgaccat aaatttgatt taatgtacgc
      481 taagagagca tttgtacact ggtacgtcgg tgagggtatg gaggaaggtg agttctccga
      541 agcacgatcc cctatagtga gtcgtattag taatcgttaa tccgcaaata acgtaaaaac
      601 ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt cagctgccaa
      661 accagatgtc aacacagcta cctgatcctt caactcagca aaagttcgat ttattcaaca
      721 aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tggataagaa
      841 atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
      901 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
      961 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtca
     1021 taaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
     1081 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
     1141 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
     1201 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
     1261 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
     1321 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
     1381 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
     1441 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
     1501 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
     1561 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
     1621 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
     1681 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
     1741 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
     1801 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
     1861 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
     1921 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
     1981 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
     2041 ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag
     2101 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
     2161 gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
     2221 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
     2281 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
     2341 tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
     2401 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
     2461 gaagaaagcc attgtcgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
     2521 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
     2581 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
     2641 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
     2701 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
     2761 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
     2821 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
     2881 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
     2941 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
     3001 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
     3061 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
     3121 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
     3181 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
     3241 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
     3301 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca
     3361 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
     3421 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
     3481 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
     3541 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
     3601 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
     3661 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
     3721 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
     3781 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
     3841 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
     3901 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
     3961 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
     4021 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
     4081 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg
     4141 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
     4201 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
     4261 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
     4321 agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
     4381 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
     4441 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
     4501 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
     4561 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
     4621 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
     4681 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
     4741 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
     4801 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
     4861 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
     4921 gctaggaggt gactgaagta tattttagat gaagattatt tcttaataac taaaaatatg
     4981 gtataatact cttaataaat gcagtaatac aggggctttt caagactgaa gtctagctga
     5041 gacaaatagt gcgattacga aattttttag acaaaaatag tctacgaggt tttagagcta
     5101 tgctgttttg aatggtccca aaacaaagca agctgataaa ccgatacaat taaaggctcc
     5161 ttttggagcc tttttttttg gagattttca acatgaagaa attattattt gatgatcaga
     5221 tagcggcggg gaactgccag acatcaaata aaacaaaagg ctcagtcgga agactgggcc
     5281 ttttgtttta tctgttgttt gtcggtgaac actctcccgg ctgagcagtt acagagatgt
     5341 tacgaacccc caggacatcc gagaatgcga ggcgatggag ggtacaaccc gagcggccgc
     5401 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     5461 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     5521 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     5581 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     5641 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     5701 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     5761 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     5821 tggctcgaag atacccgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     5881 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     5941 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     6001 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     6061 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     6121 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     6181 cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     6241 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     6301 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     6361 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     6421 gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt
     6481 ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg
     6541 gctggtcatg accaaaatcc cttaacgtga gtcagcctgc cgccttgggc cgggtgatgt
     6601 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg
     6661 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga
     6721 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac
     6781 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc
     6841 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca
     6901 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc
     6961 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta
     7021 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat
     7081 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca
     7141 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc
     7201 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc
     7261 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct
     7321 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt
     7381 gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc
     7441 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac
     7501 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg
     7561 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag
     7621 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg
     7681 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg
     7741 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt
     7801 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg
     7861 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc
     7921 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca
     7981 agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc
     8041 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc
     8101 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta
     8161 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc
     8221 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt
     8281 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc
     8341 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct
     8401 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg
     8461 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc
     8521 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg
     8581 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa
     8641 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata
     8701 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac
     8761 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
     8821 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
     8881 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
     8941 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
     9001 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
     9061 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg
     9121 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
     9181 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
     9241 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
     9301 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
     9361 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
     9421 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
     9481 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
     9541 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
     9601 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
     9661 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
     9721 tcgttcttca tcgggccacc aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg
     9781 tcaggtcgag caagagcaac gatgcgatca gcagcaccac cgtaggcatc atggaagcca
     9841 gcatcacggt tagccatagc ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg
     9901 cggcgctgct cacctcggcg gctacctccc gcaactcttt ggccagctcc acccatgccg
     9961 cccctgtctg gcgctgggct ttcagccact ccgccgcctg cgcctcgctg gcctgctggg
    10021 tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt
    10081 cgatctgctc cgctaactcg ttgatgcctc tggatttctt cactctgtcg attgcgttca
    10141 tggtctattg cctcccggta ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg
    10201 ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc cttccatctc caccacgttc
    10261 ggccccaggt gaacaccggg caggcgctcg atgccctgcg cctcaagtgt tctgtggtca
    10321 atgcgggcgt cgtggccagc ccgctctaat gcccggttgg catggtcggc ccatgcctcg
    10381 cgggtctgct caagccatgc cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc
    10441 tccggggtct tgccgttgta ccgcttgaac cactgagcgg cgggccgctc gatgccgtca
    10501 ttgatccgct cggagatcat caggtggcag tgcgggttct cgccgccacc ggcatggatg
    10561 gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc
    10621 gccttctgct ggtcgagggt cagctcgacc ggcagggcaa attcgacctc cttgaacagc
    10681 cgcccattgg cgcgttcata caggtcggca gcatcccagt agtcggcggg ccgctcgacg
    10741 aactccggca tgtgcccgga ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg
    10801 ccttcgcgct ggatgtagtc ggccttggcc ctggccgatt ggccgcccga cctgctgccg
    10861 gttttcgccg taaggtgata aatcgccatg ctgcctcgct gttgcttttg cttttcggct
    10921 ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg
    10981 aagagaaacc ggtaagtgcg ccctccccta caaagtaggg tcgggattgc cgccgctgtg
    11041 cctccatgat agcctacgag acagcacatt aacaatgggg tgtcaagatg gttaagggga
    11101 gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca acgagcgcga atcaatgccg
    11161 aaattcagcg ggtgcgggca agggaacagc agcaagagcg caagaacgaa acaaggcgca
    11221 aggtgctggt gggggccatg attttggcca aggtgaacag cagcgagtgg ccggaggatc
    11281 ggctcatggc ggcaatggat gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc
    11341 tgccgccacg ccagaaggat gagccgggct gaatgatcga ccgagacagg ccctgcgggg
    11401 ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc
    11461 tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg cctttccccc
    11521 tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc
    11581 tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga taaccgcgcc
    11641 tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc agcccccgcc
    11701 cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt gacagttatt
    11761 gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagtacggga gtgacgggca
    11821 ctggctggca atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct
    11881 aagcgataga ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg
    11941 gccaggacgg ccagccggga tcgggatact ggtcgttacc agagccaccg acccgagcaa
    12001 acccttctct atcagatcgt tgacgagtat tacccggcat tcgctgcgct tatggcagag
    12061 cagggaaagg aattgccggg ctatgtgcaa cgggaatttg aagaatttct ccaatgcggg
    12121 cggctggagc atggctttct acgggttcgc tgcgagtctt gccacgccga gcacctggtc
    12181 gctttcagaa atcaatctaa agtatatatg agtaaacttg gtctgacagg cccctgaatt
    12241 cgcatctaga ctgaactggc cgataattgc agacgaacgg acagtcattc atctttctgc
    12301 ccctccaaaa gcaaaaaccc gccgaagcgg gttttt
//
