LOCUS       sequence-215548-       13684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination vector for dox-inducible overexpression of 
            OTX2, PAX6, and MITF in AAVS locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13684)
  TITLE     TF-induced RPE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVS hOPM
FEATURES             Location/Qualifiers
     source          1..13684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(54..122,123..914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(272..291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1085..1673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1574..1593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1721..2557
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2626..2644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2662..2680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2698..2716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2734..2752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2806..2824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2869..2893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3885..3938
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5256..5309
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    6898..7019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6935..6954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6989..7008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7068..7084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7068..7084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7081..7103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7092..7108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7116..7122,7123..7140,7141..7146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7161..7182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7299..7316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7608..7627
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7626..7681
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7727..7746
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7836..8582)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8614..8633)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          8641..9658
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(8695..8717)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(9660..9937)
                     /label=chicken beta-actin promoter
     enhancer        9939..10318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    10324..10548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10537..10554
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    10570..10603
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(10879..10898)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11148..11166
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      11308..11317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             11355..11623
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(11636..11847)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    11883..12107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12128..12727)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12211..12231)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     12708..12727
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(12737..12790)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    12814..12839
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    12846..12879
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    12881..13684
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       61 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1201 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1681 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
     1741 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
     1801 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
     1861 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
     1921 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
     1981 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
     2041 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
     2101 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
     2161 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
     2221 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
     2281 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
     2341 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
     2401 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
     2461 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
     2521 aggatggggc ttttctgtca ccaatcctgt ccctagtaaa gctgatatct ccaccccaca
     2581 gtggtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2641 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2701 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     2761 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     2821 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2881 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta
     2941 ccacttccta ccctcgtaaa cttaaggtta attaagccgc caccatgatg tcttatctta
     3001 agcaaccgcc ttacgcagtc aatgggctga gtctgaccac ttcgggtatg gacttgctgc
     3061 acccctccgt gggctacccg gggccctggg cttcttgtcc cgcagccacc ccccggaaac
     3121 agcgccggga gaggacgacg ttcactcggg cgcagctaga tgtgctggaa gcactgtttg
     3181 ccaagacccg gtacccagac atcttcatgc gagaggaggt ggcactgaaa atcaacttgc
     3241 ccgagtcgag ggtgcaggta tggtttaaga atcgaagagc taagtgccgc caacaacagc
     3301 aacaacagca gaatggaggt caaaacaaag tgagacctgc caaaaagaag acatctccag
     3361 ctcgggaagt gagttcagag agtggaacaa gtggccaatt cactcccccc tctagcacct
     3421 cagtcccgac cattgccagc agcagtgctc ctgtgtctat ctggagccca gcttccatct
     3481 ccccactgtc agatcccttg tccacctcct cttcctgcat gcagaggtcc tatcccatga
     3541 cctatactca ggcttcaggt tatagtcaag gatatgctgg ctcaacttcc tactttgggg
     3601 gcatggactg tggatcatat ttgaccccta tgcatcacca gcttcccgga ccaggggcca
     3661 cactcagtcc catgggtacc aatgcagtca ccagccatct caatcagtcc ccagcttctc
     3721 tttccaccca gggatatgga gcttcaagct tgggttttaa ctcaaccact gattgcttgg
     3781 attataagga ccaaactgcc tcctggaagc ttaacttcaa tgctgactgc ttggattata
     3841 aagatcagac atcctcgtgg aaattccagg ttttgggatc gggagagggc agaggaagtc
     3901 tgctaacatg cggtgacgtc gaggagaatc ctggcccaat gcagaacagt cacagcggag
     3961 tgaatcagct cggtggtgtc tttgtcaacg ggcggccact gccggactcc acccggcaga
     4021 agattgtaga gctagctcac agcggggccc ggccgtgcga catttcccga attctgcaga
     4081 cccatgcaga tgcaaaagtc caagtgctgg acaatcaaaa cgtgtccaac ggatgtgtga
     4141 gtaaaattct gggcaggtat tacgagactg gctccatcag acccagggca atcggtggta
     4201 gtaaaccgag agtagcgact ccagaagttg taagcaaaat agcccagtat aagcgggagt
     4261 gcccgtccat ctttgcttgg gaaatccgag acagattact gtccgagggg gtctgtacca
     4321 acgataacat accaagcgtg tcatcaataa acagagttct tcgcaacctg gctagcgaaa
     4381 agcaacagat gggcgcagac ggcatgtatg ataaactaag gatgttgaac gggcagaccg
     4441 gaagctgggg cacccgccct ggttggtatc cggggacttc ggtgccaggg caacctacgc
     4501 aagatggctg ccagcaacag gaaggagggg gagagaatac caactccatc agttccaacg
     4561 gagaagattc agatgaggct caaatgcgac ttcagctgaa gcggaagctg caaagaaata
     4621 gaacatcctt tacccaagag caaattgagg ccctggagaa agagtttgag agaacccatt
     4681 atccagatgt gtttgcccga gaaagactag cagccaaaat agatctacct gaagcaagaa
     4741 tacaggtatg gttttctaat cgaagggcca aatggagaag agaagaaaaa ctgaggaatc
     4801 agagaagaca ggccagcaac acacctagtc atattcctat cagcagtagt ttcagcacca
     4861 gtgtctacca accaattcca caacccacca caccggtttc ctccttcaca tctggctcca
     4921 tgttgggccg aacagacaca gccctcacaa acacctacag cgctctgccg cctatgccca
     4981 gcttcaccat ggcaaataac ctgcctatgc aacccccagt ccccagccag acctcctcat
     5041 actcctgcat gctgcccacc agcccttcgg tgaatgggcg gagttatgat acctacaccc
     5101 ccccacatat gcagacacac atgaacagtc agccaatggg cacctcgggc accacttcaa
     5161 caggactcat ttcccctggt gtgtcagttc cagttcaagt tcccggaagt gaacctgata
     5221 tgtctcaata ctggccaaga ttacagggat cgggagaggg cagaggaagt ctgctaacat
     5281 gcggtgacgt cgaggagaat cctggcccaa tgcagtccga atcggggatc gtgccggatt
     5341 tcgaagtcgg ggaggagttt catgaagagc ccaaaaccta ttacgaactc aaaagtcaac
     5401 cgctgaagag cagcagttcc gccgagcatc ctggggcctc caagcctccg ataagctcct
     5461 ccagtatgac atcacgcatc ttgctacgcc agcaactcat gcgtgagcag atgcaggagc
     5521 aggagcgcag ggagcagcag cagaagctgc aggcggccca gttcatgcaa cagagagtgc
     5581 ccgtgagtca gacaccagcc ataaacgtca gtgtgcccac cacccttccc tctgccacgc
     5641 aggtgccgat ggaagtcctt aaggtgcaga cccacctcga aaaccccacc aagtaccaca
     5701 tacagcaagc ccaacggcag caggtaaagc agtacctttc taccacttta gcaaataaac
     5761 atgccaacca agtcctgagc ttgccatgtc caaaccagcc tggcgatcat gtcatgccac
     5821 cggtgccggg gagcagcgca cccaacagcc ccatggctat gcttacgctt aactccaact
     5881 gtgaaaaaga gggattttat aagtttgaag agcaaaacag ggcagagagc gagtgcccag
     5941 gcatgaacac acattcacga gcgtcctgta tgcagatgga tgatgtaatc gatgacatca
     6001 ttagcctaga atcaagttat aatgaggaaa tcttgggctt gatggatcct gctttgcaaa
     6061 tggcaaatac gttgcctgtc tcgggaaact tgattgatct ttatggaaac caaggtctgc
     6121 ccccaccagg cctcaccatc agcaactcct gtccagccaa ccttcccaac ataaaaaggg
     6181 agctcacaga gtctgaagca agagcactgg ccaaagagag gcagaaaaag gacaatcaca
     6241 acctgattga acgaagaaga agatttaaca taaatgaccg cattaaagaa ctaggtactt
     6301 tgattcccaa gtcaaatgat ccagacatgc gctggaacaa gggaaccatc ttaaaagcat
     6361 ccgtggacta tatccgaaag ttgcaacgag aacagcaacg cgcaaaagaa cttgaaaacc
     6421 gacagaagaa actggagcac gccaaccggc atttgttgct cagaatacag gaacttgaaa
     6481 tgcaggctcg agctcatgga ctttccctta ttccatccac gggtctctgc tctccagatt
     6541 tggtgaatcg gatcatcaag caagaacccg ttcttgagaa ctgcagccaa gacctccttc
     6601 agcatcatgc agacctaacc tgtacaacaa ctctcgatct cacggatggc accatcacct
     6661 tcaacaacaa cctcggaact gggactgagg ccaaccaagc ctatagtgtc cccacaaaaa
     6721 tgggatccaa actggaagac atcctgatgg acgacaccct ttctcccgtc ggtgtcactg
     6781 atccactcct ttcctcagtg tcccccggag cttccaaaac aagcagccgg aggagcagta
     6841 tgagcatgga agagacggag cacacttgtt aggcggccgc aatcgatcgc ccaccccaac
     6901 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7021 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggacggccg cacatgtaag
     7381 gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa aaaaatctaa
     7441 cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg tttcatatac
     7501 tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc atatgttgcc
     7561 aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc atatgtcctt
     7621 ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa tttcctttat
     7681 tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt ggcagaggga
     7741 aaaagatctc agtggtattt gtgagccagg gcattggcca caccagccac caccttctga
     7801 taggcagcct gcacctgagg agtgaattca cgcgtttagc ctggcagcat atcgagatcg
     7861 aagtcatcga gagcgtcagc agggagcata tccagatcaa aatcatccag ggcatcggcg
     7921 ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggtgg gtccgccgct ctcgcacttc
     7981 agctgcttct cgaggccgca gatgatcagt tccaggccga acaggaaggc gggctcggcg
     8041 ccctgtctgt cgaacagctc gatggcctgc ttcagcaggg ggggcatgct gtcggtggtg
     8101 ggtgtctctc tttcctcttt ggcgacctgg tgttcctgtt cttccagcac gcagcccagg
     8161 gtgaagtggc ccacggcgct cagggcgtac agggcgtttt ccaggctgaa gccctgctgg
     8221 cacaggaagg ccagctggtt ttccagtgtc tcgtactgct tctcggtggg tctggtgccc
     8281 aggtgcactt tggcgccgtc ccggtggctc agcagggcgc atctgtagct cttggcgttg
     8341 ttccgcagga agtcctgcca gctctcgcct tccagagggc agctgtgggt gtggtgccgg
     8401 tccagcatct cgatgggcag ggcgtccagc agggcccgct tgttcttcac gtgccagtac
     8461 agggtgggct gttccacgcc cagtttctgg gccagcttcc gggtggtcag gccctcgatg
     8521 cccacgccgt tcagcagttc cagggcgctg ttgatcactt tgctcttgtc cagccggctc
     8581 atgtcgacat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
     8641 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
     8701 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
     8761 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
     8821 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
     8881 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
     8941 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc
     9001 ggcgctccgg gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc
     9061 cccgcccggc acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg
     9121 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg
     9181 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac
     9241 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc
     9301 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     9361 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca
     9421 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
     9481 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
     9541 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     9601 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgtcgca gcgactcccc
     9661 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
     9721 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
     9781 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc
     9841 gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag
     9901 atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat
     9961 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
    10021 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
    10081 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
    10141 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
    10201 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
    10261 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
    10321 gacccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
    10381 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
    10441 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
    10501 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
    10561 agcgggttta taacttcgta taatgtatgc tatacgaagt tatggtacct tactcgtcca
    10621 tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct
    10681 cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg
    10741 gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct
    10801 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
    10861 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
    10921 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
    10981 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
    11041 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
    11101 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca
    11161 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
    11221 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
    11281 ccatgttatc ctcctcgccc ttgctcacca tggtggcgac cggtagcgct agcgtaggcg
    11341 ccggtcacag cttggatctg taacggcgca gaacagaaaa cgaaacaaag acgtagagtt
    11401 gagcaagcag ggtcaggcaa agcgtggaga gccggctgag tctaggtagg ctccaaggga
    11461 gcgccggaca aaggcccggt ctcgacctga gctttaaact tacctagacg gcggacgcag
    11521 ttcaggaggc accacaggcg ggaggcggca gaacgcgact caaccggcgt ggatggcggc
    11581 ctcaggtagg gcggcgggcg cgtgaaggag agatgcgagc ccctcgaagc ttcagctgtg
    11641 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg
    11701 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta
    11761 ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg
    11821 ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcgatcg cagatcatgc
    11881 atccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
    11941 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
    12001 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
    12061 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacaggtt taaacgggcc
    12121 ctctagatca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
    12181 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
    12241 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
    12301 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
    12361 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
    12421 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
    12481 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
    12541 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
    12601 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
    12661 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
    12721 cggtcatctc gagcctaggg ccgggattct cctccacgtc accgcatgtt agaagacttc
    12781 ctctgccctc tccgctgcca gatctctcga ggccctgtgg gaggaagaga agaggtcaga
    12841 agcttataac ttcgtataat gtatgctata cgaagttatt gccccactgt ggggtggagg
    12901 ggacagataa aagtacccag aaccagagcc acattaaccg gccctgggaa tataaggtgg
    12961 tcccagctcg gggacacagg atccctggag gcagcaaaca tgctgtcctg aagtggacat
    13021 aggggcccgg gttggaggaa gaagactagc tgagctctcg gacccctgga agatgccatg
    13081 acagggggct ggaagagcta gcacagacta gagaggtaag gggggtaggg gagctgccca
    13141 aatgaaagga gtgagaggtg acccgaatcc acaggagaac ggggtgtcca ggcaaagaaa
    13201 gcaagaggat ggagaggtgg ctaaagccag ggagacgggg tactttgggg ttgtccagaa
    13261 aaacggtgat gatgcaggcc tacaagaagg ggaggcggga cgcaagggag acatccgtcg
    13321 gagaaggcca tcctaagaaa cgagagatgg cacaggcccc agaaggagaa ggaaaaggga
    13381 acccagcgag tgaagacggc atggggttgg gtgagggagg agagatgccc ggagaggacc
    13441 cagacacggg gaggatccgc tcagaggaca tcacgtggtg cagcgccgag aaggaagtgc
    13501 tccggaaaga gcatccttgg gcagcaacac agcagagagc aaggggaaga gggagtggag
    13561 gaagacggaa cctgaaggag gcggcaggga aggatctggg ccagccgtag aggtgaccca
    13621 ggccacaagc tgcagacaga aagcggcaca ggcccagggg agagaatgct ggtcagagaa
    13681 agca
//
