LOCUS       sequence-21555-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Mena MA, Treynor TP, Mayo SL, Daugherty PS
  TITLE     Blue fluorescent proteins with enhanced brightness and 
            photostability from a structurally targeted library.
  JOURNAL   Nat Biotechnol. 2006 Dec . 24(12):1569-71.
  PUBMED    17115054
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2006 Dec . 24(12):1569-71."
COMMENT     Sequence Label: pCEP4Azurite-Mamm
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(381..400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctcg tttagtggac cgtcagatct ctagaagctg ggtaccgcgg gcccgggatc
       61 caccggtcgc caccatggtg agcaaaggcg aagagctgtt caccggcgtg gtgcccatcc
      121 tggtggagct ggacggcgac gtgaacggcc acaagttcag cgtgagcggc gagggcgagg
      181 gcgacgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      241 tgccctggcc caccctggtg accaccctga gccacggcgt gcagtgcttc agccggtacc
      301 ccgaccacat gaagcagcac gacttcttca agagcgccat gcccgagggc tacgtgcagg
      361 agcggaccat cttcttcaag gacgacggca actacaagac ccgggccgag gtgaagttcg
      421 agggcgacac cctggtgaac cggatcgagc tgaagggcat cgacttcaag gaggacggca
      481 acatcctggg ccacaagctg gagtacaact tcaacagcca caacatctac atcatggccg
      541 acaagcagaa gaacggcatc aaggtgaact tcaagatccg gcacaacatc gaggacggca
      601 gcgtgcagct ggccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      661 tgcccgacaa ccactacctg agcacccaga gcgccctgag caaggacccc aacgagaagc
      721 gggaccacat ggtgctgctg g
//
