LOCUS       sequence-215550-       14459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination vector for dox-inducible overexpression of 
            OTX2, PAX6, and MITF in CLYBL locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14459)
  TITLE     TF-induced RPE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CLYBL hOPM
FEATURES             Location/Qualifiers
     source          1..14459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(395..1186,1187..1255))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1018..1037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    complement(2124..2157)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2164..2189
                     /label=SA
                     /note="splice acceptor site"
     CDS             2213..2266
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2276..2875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2276..2295)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2772..2792
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2896..3120
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3156..3367
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3380..3648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3686..3695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3837..3855)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4105..4124
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4400..4433)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4449..4466)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4455..4679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4685..5064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5066..5343
                     /label=chicken beta-actin promoter
     intron          5345..6362
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6286..6308
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6370..6389
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6421..7167
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7257..7276)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7322..7377
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7376..7395)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     7687..7704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7821..7842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7857..7862,7863..7880,7881..7887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7895..7911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7900..7922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7919..7935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7919..7935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7984..8105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7995..8014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8049..8068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9691..9744)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(11062..11115)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(12031..12408)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(12098..12122)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    12167..12185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12203..12221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12239..12257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12275..12293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12311..12329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12347..12365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12383..12401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     rep_origin      complement(join(14095..14459,1..224))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14175..14194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
       61 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1261 tcctttttca atagcaggag aatcatgcca atgggccaat atacattctg acccacagtt
     1321 tcataataaa ataaaatggt tgtggttgta agccactatg tttcagagtg gtttgttaca
     1381 cagcaataaa taactaatat agtaggcata ccatcaagtc caaagtaggt agagaagaat
     1441 gtaaatagca gagcaaaaca gcatgactgg tggctgggag gcttaaaact gggacaggat
     1501 cagagtcatg aaagaagtca aagaaatggt tcagaagtaa ggctgagact gacttacaaa
     1561 agctgaaagt ccctttaagt tggtgtttgg tgcattggca ggggcaggta tggtgactta
     1621 aaagagccat gctcaacaag atcaagcaca acacaatcac gggtcacccc agcagacctt
     1681 agcgagtcta gccatttctt tggtggtggt cacagtcatg cttcagccca gtttccactt
     1741 ggacaaatgg tacatatttt caatgagatg aaaattaaga tacaatccat gtgctcagag
     1801 agtgatcaca gctctgacta aacactgtgc cccaaagtgt tgaggaattg ggaaaaccta
     1861 gctgagttag tggtctcttt tctgttacaa taaagctcat aatgaaaatt agccttcttt
     1921 gttcttcccc aagtctttct ttctagacga aactactttc aactgtttta acttccttac
     1981 tgttaacttc catatttcta gatagtatgg tcagactgtt atttcttgac ttttaaatgt
     2041 aagatattat ctactgactt ccttctatgt aagatgagga ttgagctctc ttacccttct
     2101 cccagcggcc ccacagtggg gcaataactt cgtatagcat acattatacg aagttataag
     2161 cttctgacct cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag
     2221 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctaggc tcgagatgac
     2281 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2341 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     2401 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     2461 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     2521 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     2581 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     2641 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     2701 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     2761 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     2821 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctag
     2881 agggcccgtt taaacctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2941 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3001 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3061 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3121 atgcatgatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     3181 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     3241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3361 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     3421 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     3481 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     3541 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     3601 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     3661 ggcgcctacg ctagcgctac cggtcgccac catggtgagc aagggcgagg aggataacat
     3721 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3781 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3841 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3901 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3961 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4021 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4081 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4141 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4201 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4261 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4321 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4381 catggacgag taaggtacca taacttcgta tagcatacat tatacgaagt tataaacccg
     4441 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4501 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4561 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4621 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg
     4681 tctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4741 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4801 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4861 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4921 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4981 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     5041 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     5101 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     5161 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     5221 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     5281 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     5341 gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     5401 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     5461 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     5521 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     5581 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     5641 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     5701 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     5761 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     5821 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     5881 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     5941 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     6001 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     6061 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     6121 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     6181 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     6241 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     6301 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     6361 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatgtcgac
     6421 atgagccggc tggacaagag caaagtgatc aacagcgccc tggaactgct gaacggcgtg
     6481 ggcatcgagg gcctgaccac ccggaagctg gcccagaaac tgggcgtgga acagcccacc
     6541 ctgtactggc acgtgaagaa caagcgggcc ctgctggacg ccctgcccat cgagatgctg
     6601 gaccggcacc acacccacag ctgccctctg gaaggcgaga gctggcagga cttcctgcgg
     6661 aacaacgcca agagctacag atgcgccctg ctgagccacc gggacggcgc caaagtgcac
     6721 ctgggcacca gacccaccga gaagcagtac gagacactgg aaaaccagct ggccttcctg
     6781 tgccagcagg gcttcagcct ggaaaacgcc ctgtacgccc tgagcgccgt gggccacttc
     6841 accctgggct gcgtgctgga agaacaggaa caccaggtcg ccaaagagga aagagagaca
     6901 cccaccaccg acagcatgcc ccccctgctg aagcaggcca tcgagctgtt cgacagacag
     6961 ggcgccgagc ccgccttcct gttcggcctg gaactgatca tctgcggcct cgagaagcag
     7021 ctgaagtgcg agagcggcgg acccaccgac gccctggacg acttcgacct ggacatgctg
     7081 cccgccgatg ccctggatga ttttgatctg gatatgctcc ctgctgacgc tctcgatgac
     7141 ttcgatctcg atatgctgcc aggctaaacg cgtgaattca ctcctcaggt gcaggctgcc
     7201 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     7261 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     7321 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     7381 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     7441 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     7501 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     7561 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     7621 tccttacatg tgcggccgtc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7921 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca
     7981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     8041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     8101 aagttggggt gggcgatcga ttcgcgacta acaagtgtgc tccgtctctt ccatgctcat
     8161 actgctcctc cggctgcttg ttttggaagc tccgggggac actgaggaaa ggagtggatc
     8221 agtgacaccg acgggagaaa gggtgtcgtc catcaggatg tcttccagtt tggatcccat
     8281 ttttgtgggg acactatagg cttggttggc ctcagtccca gttccgaggt tgttgttgaa
     8341 ggtgatggtg ccatccgtga gatcgagagt tgttgtacag gttaggtctg catgatgctg
     8401 aaggaggtct tggctgcagt tctcaagaac gggttcttgc ttgatgatcc gattcaccaa
     8461 atctggagag cagagacccg tggatggaat aagggaaagt ccatgagctc gagcctgcat
     8521 ttcaagttcc tgtattctga gcaacaaatg ccggttggcg tgctccagtt tcttctgtcg
     8581 gttttcaagt tcttttgcgc gttgctgttc tcgttgcaac tttcggatat agtccacgga
     8641 tgcttttaag atggttccct tgttccagcg catgtctgga tcatttgact tgggaatcaa
     8701 agtacctagt tctttaatgc ggtcatttat gttaaatctt cttcttcgtt caatcaggtt
     8761 gtgattgtcc tttttctgcc tctctttggc cagtgctctt gcttcagact ctgtgagctc
     8821 cctttttatg ttgggaaggt tggctggaca ggagttgctg atggtgaggc ctggtggggg
     8881 cagaccttgg tttccataaa gatcaatcaa gtttcccgag acaggcaacg tatttgccat
     8941 ttgcaaagca ggatccatca agcccaagat ttcctcatta taacttgatt ctaggctaat
     9001 gatgtcatcg attacatcat ccatctgcat acaggacgct cgtgaatgtg tgttcatgcc
     9061 tgggcactcg ctctctgccc tgttttgctc ttcaaactta taaaatccct ctttttcaca
     9121 gttggagtta agcgtaagca tagccatggg gctgttgggt gcgctgctcc ccggcaccgg
     9181 tggcatgaca tgatcgccag gctggtttgg acatggcaag ctcaggactt ggttggcatg
     9241 tttatttgct aaagtggtag aaaggtactg ctttacctgc tgccgttggg cttgctgtat
     9301 gtggtacttg gtggggtttt cgaggtgggt ctgcacctta aggacttcca tcggcacctg
     9361 cgtggcagag ggaagggtgg tgggcacact gacgtttatg gctggtgtct gactcacggg
     9421 cactctctgt tgcatgaact gggccgcctg cagcttctgc tgctgctccc tgcgctcctg
     9481 ctcctgcatc tgctcacgca tgagttgctg gcgtagcaag atgcgtgatg tcatactgga
     9541 ggagcttatc ggaggcttgg aggccccagg atgctcggcg gaactgctgc tcttcagcgg
     9601 ttgacttttg agttcgtaat aggttttggg ctcttcatga aactcctccc cgacttcgaa
     9661 atccggcacg atccccgatt cggactgcat tgggccagga ttctcctcga cgtcaccgca
     9721 tgttagcaga cttcctctgc cctctcccga tccctgtaat cttggccagt attgagacat
     9781 atcaggttca cttccgggaa cttgaactgg aactgacaca ccaggggaaa tgagtcctgt
     9841 tgaagtggtg cccgaggtgc ccattggctg actgttcatg tgtgtctgca tatgtggggg
     9901 ggtgtaggta tcataactcc gcccattcac cgaagggctg gtgggcagca tgcaggagta
     9961 tgaggaggtc tggctgggga ctgggggttg cataggcagg ttatttgcca tggtgaagct
    10021 gggcataggc ggcagagcgc tgtaggtgtt tgtgagggct gtgtctgttc ggcccaacat
    10081 ggagccagat gtgaaggagg aaaccggtgt ggtgggttgt ggaattggtt ggtagacact
    10141 ggtgctgaaa ctactgctga taggaatatg actaggtgtg ttgctggcct gtcttctctg
    10201 attcctcagt ttttcttctc ttctccattt ggcccttcga ttagaaaacc atacctgtat
    10261 tcttgcttca ggtagatcta ttttggctgc tagtctttct cgggcaaaca catctggata
    10321 atgggttctc tcaaactctt tctccagggc ctcaatttgc tcttgggtaa aggatgttct
    10381 atttctttgc agcttccgct tcagctgaag tcgcatttga gcctcatctg aatcttctcc
    10441 gttggaactg atggagttgg tattctctcc ccctccttcc tgttgctggc agccatcttg
    10501 cgtaggttgc cctggcaccg aagtccccgg ataccaacca gggcgggtgc cccagcttcc
    10561 ggtctgcccg ttcaacatcc ttagtttatc atacatgccg tctgcgccca tctgttgctt
    10621 ttcgctagcc aggttgcgaa gaactctgtt tattgatgac acgcttggta tgttatcgtt
    10681 ggtacagacc ccctcggaca gtaatctgtc tcggatttcc caagcaaaga tggacgggca
    10741 ctcccgctta tactgggcta ttttgcttac aacttctgga gtcgctactc tcggtttact
    10801 accaccgatt gccctgggtc tgatggagcc agtctcgtaa tacctgccca gaattttact
    10861 cacacatccg ttggacacgt tttgattgtc cagcacttgg acttttgcat ctgcatgggt
    10921 ctgcagaatt cgggaaatgt cgcacggccg ggccccgctg tgagctagct ctacaatctt
    10981 ctgccgggtg gagtccggca gtggccgccc gttgacaaag acaccaccga gctgattcac
    11041 tccgctgtga ctgttctgca ttgggccagg attctcctcg acgtcaccgc atgttagcag
    11101 acttcctctg ccctctcccg atcccaaaac ctggaatttc cacgaggatg tctgatcttt
    11161 ataatccaag cagtcagcat tgaagttaag cttccaggag gcagtttggt ccttataatc
    11221 caagcaatca gtggttgagt taaaacccaa gcttgaagct ccatatccct gggtggaaag
    11281 agaagctggg gactgattga gatggctggt gactgcattg gtacccatgg gactgagtgt
    11341 ggcccctggt ccgggaagct ggtgatgcat aggggtcaaa tatgatccac agtccatgcc
    11401 cccaaagtag gaagttgagc cagcatatcc ttgactataa cctgaagcct gagtataggt
    11461 catgggatag gacctctgca tgcaggaaga ggaggtggac aagggatctg acagtgggga
    11521 gatggaagct gggctccaga tagacacagg agcactgctg ctggcaatgg tcgggactga
    11581 ggtgctagag gggggagtga attggccact tgttccactc tctgaactca cttcccgagc
    11641 tggagatgtc ttctttttgg caggtctcac tttgttttga cctccattct gctgttgttg
    11701 ctgttgttgg cggcacttag ctcttcgatt cttaaaccat acctgcaccc tcgactcggg
    11761 caagttgatt ttcagtgcca cctcctctcg catgaagatg tctgggtacc gggtcttggc
    11821 aaacagtgct tccagcacat ctagctgcgc ccgagtgaac gtcgtcctct cccggcgctg
    11881 tttccggggg gtggctgcgg gacaagaagc ccagggcccc gggtagccca cggaggggtg
    11941 cagcaagtcc atacccgaag tggtcagact cagcccattg actgcgtaag gcggttgctt
    12001 aagataagac atcatggtgg cggcggatcc tttacgaggg taggaagtgg tacggaaagt
    12061 tggtataaga caaaagtgtt gtggaattgc tccaggcgat ctgacggttc actaaacgag
    12121 ctctgctttt ataggcgccc accgtacacg cctaaagctt atacgttctc tatcactgat
    12181 agggagtaaa ctggatatac gttctctatc actgataggg agtaaactgt agatacgttc
    12241 tctatcactg atagggagta aactggtcat acgttctcta tcactgatag ggagtaaact
    12301 ccttatacgt tctctatcac tgatagggag taaagtctgc atacgttctc tatcactgat
    12361 agggagtaaa ctcttcatac gttctctatc actgataggg agtaaactgg taccctccgc
    12421 gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg cggggccgcc
    12481 ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca gcgggcgcgc
    12541 gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc tagggggagg
    12601 gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg tggtccccaa
    12661 ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag cggtggcttt
    12721 tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt cctcgcccct
    12781 gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg agcttaaaaa
    12841 ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag gggtcagttc
    12901 cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac tagaagttga
    12961 ttcgggtgtt tccggaaggg gccgagtcaa tccgccgagt tggggcacgg aaaacaaaaa
    13021 gggaaggcta ctaagatttt tctggcgggg gttatcattg gcgtaactgc agggaccacc
    13081 tcccttaatt aaatctcctc atccttccaa cataaatata ttttgggatt atatcaacat
    13141 tcaatgttac ttaaagtgac cttgtaaata ttttcacaac tgagccatgt ttgatttgta
    13201 tacttatgtt tactttactg tttttcctga agttaataat tgccttgaat ttatttattt
    13261 ctttaaaaat gtttcattac tcaggactgt agtttacatt acgattcttt gtgttataca
    13321 gttgatgggt ttcttttctt tcttaatttc tttaaaaaat agagatgggg tcttactata
    13381 ttacccaggc tggtcttgaa gtcctgggct caagtgatct tcctgtctca gcctaccaag
    13441 tagctgagac tataggtgca aaaaagccac tatacctggc tagtttacag gttttaacaa
    13501 atgcattatg ccacgtatcc attattacag gatcacacaa gatattttca ttaccctgaa
    13561 agcatccctg tgttccacca attcatcctg cctccatgag ccgctggcaa ccactgatct
    13621 ctatagtttt gccttttcta aaatgtcata taattggaat catacagtct gtagcatttt
    13681 cagactagct tttaaaattt ggcaatatgc atttaaggtt cctccttaaa tgtgaagggc
    13741 atagccaatg tggcttgata gctcatttct ttttattggt gaatatttca ttgtctggat
    13801 gttccacagt ttgtttatcc attcacctat tcaatttgct ttttttctgt gtatctatca
    13861 ctaattcaat actggactct ccaacagagc cgtcccgctt ccctaaggcc ctgctctggg
    13921 cttctgggtt tgagtccttg gcaagcccag gagaggcgct caggcttccc tgtccccctt
    13981 cctcgtccac catctcatgc ccctggctct cctgcccctt ccctacaggg gttcctggct
    14041 ctgctctaag ggcgaattcg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    14101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    14161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    14221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    14281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    14341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    14401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaaca
//
