LOCUS       sequence-215556-        9910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized AsBE1.4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV
            40)(AsBE1.4) (RTW1008)
FEATURES             Location/Qualifiers
     source          1..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1775..1795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1805..1825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1835..2518
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2615..6532
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6563..6811
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6824..6844
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6934..6953)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6999..7054
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7053..7072)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7413..7429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7413..7429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7426..7448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7437..7453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7461..7467,7468..7485,7486..7491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7506..7527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7586..7781
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7632..7767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7787..7921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7824..7843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7878..7897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7989..8006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8160..8748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8240..8259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8919..9710,9711..9779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9542..9561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9780..9884)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcccaaag aaaaagagga aagtcggcag
     1801 tggacctaaa aagaaacgaa aggttgggtc aggtagctca gagactggcc cagtggctgt
     1861 ggaccccaca ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag
     1921 agagctccgc aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat
     1981 ttggcgacat acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt
     2041 cacgacagaa agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg
     2101 gagcccatgc ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt
     2161 cactctgttt atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg
     2221 cctgcgggat ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg
     2281 atactgctgg agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta
     2341 tccccatctg tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc
     2401 tccttgtctc aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct
     2461 tcagtcttgt cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaatc
     2521 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac
     2581 acccgaaagt tccggaggga gtagcggcgg gtctacacag ttcgagggct ttaccaacct
     2641 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
     2701 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
     2761 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
     2821 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
     2881 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
     2941 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
     3001 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
     3061 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
     3121 cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
     3181 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
     3241 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
     3301 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
     3361 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
     3421 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
     3481 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
     3541 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
     3601 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
     3661 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
     3721 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
     3781 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
     3841 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
     3901 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
     3961 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
     4021 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
     4081 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
     4141 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
     4201 gttcaagctg aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa
     4261 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
     4321 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
     4381 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
     4441 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
     4501 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
     4561 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
     4621 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
     4681 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
     4741 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
     4801 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
     4861 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
     4921 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
     4981 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
     5041 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
     5101 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
     5161 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt
     5221 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
     5281 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgcccgggg
     5341 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
     5401 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
     5461 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
     5521 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
     5581 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
     5641 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
     5701 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
     5761 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
     5821 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
     5881 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
     5941 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
     6001 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
     6061 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
     6121 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
     6181 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
     6241 cgccatcgac acgatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
     6301 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
     6361 ctcccggttt cagaacccag agtggccaat ggacgccgat gccaatggcg cctaccacat
     6421 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
     6481 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca actctggtgg
     6541 ttctggagga tctggtggtt ctactaatct gtcagatatt attgaaaagg agaccggtaa
     6601 gcaactggtt atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg
     6661 gaacaagccg gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa
     6721 tgtcatgctt ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga
     6781 tagcaacggt gagaacaaga ttaagatgct ctctggtggt tctcccaaga agaagaggaa
     6841 agtctaaagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     6901 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     6961 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     7021 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     7081 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg
     7141 gctgccatga acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     7201 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     7261 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     7321 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc
     7381 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7441 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7501 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7561 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
     7621 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7681 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7741 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     7801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     7921 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8341 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8581 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9901 gtgccacctg
//
