LOCUS       sequence-215557-        9904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized enAsBE1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(n
            ucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)
FEATURES             Location/Qualifiers
     source          1..9904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1769..2458
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MGSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     GGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     NEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     ILWATGLK"
     CDS             2555..6472
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6473..6520
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6557..6805
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6818..6838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6928..6947)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6993..7048
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7047..7066)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7407..7423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7407..7423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7420..7442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7431..7447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7455..7461,7462..7479,7480..7485))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7500..7521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7580..7775
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7626..7761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7781..7915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7818..7837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7872..7891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7983..8000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8154..8742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8234..8253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8913..9704,9705..9773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9536..9555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9774..9878)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcagctca gagactggcc cagtggctgt
     1801 ggaccccaca ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag
     1861 agagctccgc aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat
     1921 ttggcgacat acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt
     1981 cacgacagaa agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg
     2041 gagcccatgc ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt
     2101 cactctgttt atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg
     2161 cctgcgggat ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg
     2221 atactgctgg agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta
     2281 tccccatctg tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc
     2341 tccttgtctc aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct
     2401 tcagtcttgt cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaatc
     2461 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac
     2521 acccgaaagt tccggaggga gtagcggcgg gtctacacag ttcgagggct ttaccaacct
     2581 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
     2641 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
     2701 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
     2761 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
     2821 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
     2881 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
     2941 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
     3001 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
     3061 cggcttttat agaaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
     3121 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
     3181 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
     3241 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
     3301 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
     3361 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
     3421 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
     3481 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
     3541 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
     3601 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
     3661 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
     3721 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
     3781 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
     3841 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
     3901 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
     3961 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
     4021 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
     4081 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
     4141 gttcaagctg aactttcaga tgcctacact ggccagaggc tgggacgtga atagagagaa
     4201 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
     4261 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
     4321 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
     4381 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
     4441 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
     4501 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
     4561 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
     4621 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
     4681 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
     4741 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
     4801 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
     4861 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
     4921 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
     4981 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
     5041 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
     5101 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt
     5161 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
     5221 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgcccgggg
     5281 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
     5341 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
     5401 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
     5461 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
     5521 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
     5581 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
     5641 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
     5701 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
     5761 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
     5821 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
     5881 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
     5941 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
     6001 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
     6061 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
     6121 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
     6181 cgccatcgac acgatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
     6241 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
     6301 ctcccggttt cagaacccag agtggccaat ggacgccgat gccaatggcg cctaccacat
     6361 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
     6421 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc
     6481 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctctg gtggttctgg
     6541 aggatctggt ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact
     6601 ggttatccag gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa
     6661 gccggaaagc gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat
     6721 gcttctgact agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa
     6781 cggtgagaac aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcta
     6841 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     6901 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     6961 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     7021 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     7081 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     7141 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     7201 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     7261 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     7321 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc
     7381 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7441 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7501 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7561 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     7621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7741 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     7921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8341 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8581 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9901 cctg
//
