LOCUS       sequence-215564-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized LbBE1.4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV
            40)(LbBE1.4) (RTW1293)
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1775..1795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1805..1825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1835..2518
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2615..6295
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6326..6574
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6587..6607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6697..6716)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6762..6817
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6816..6835)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7176..7192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7176..7192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7189..7211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7200..7216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7224..7230,7231..7248,7249..7254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7269..7290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7349..7544
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7395..7530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7550..7684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7587..7606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7641..7660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7752..7769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7923..8511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8003..8022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8682..9473,9474..9542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9305..9324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9543..9647)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcccaaag aaaaagagga aagtcggcag
     1801 tggacctaaa aagaaacgaa aggttgggtc aggtagctca gagactggcc cagtggctgt
     1861 ggaccccaca ttgagacggc ggatcgagcc ccatgagttt gaggtattct tcgatccgag
     1921 agagctccgc aaggagacct gcctgcttta cgaaattaat tgggggggcc ggcactccat
     1981 ttggcgacat acatcacaga acactaacaa gcacgtcgaa gtcaacttca tcgagaagtt
     2041 cacgacagaa agatatttct gtccgaacac aaggtgcagc attacctggt ttctcagctg
     2101 gagcccatgc ggcgaatgta gtagggccat cactgaattc ctgtcaaggt atccccacgt
     2161 cactctgttt atttacatcg caaggctgta ccaccacgct gacccccgca atcgacaagg
     2221 cctgcgggat ttgatctctt caggtgtgac tatccaaatt atgactgagc aggagtcagg
     2281 atactgctgg agaaactttg tgaattatag cccgagtaat gaagcccact ggcctaggta
     2341 tccccatctg tgggtacgac tgtacgttct tgaactgtac tgcatcatac tgggcctgcc
     2401 tccttgtctc aacattctga gaaggaagca gccacagctg acattcttta ccatcgctct
     2461 tcagtcttgt cattaccagc gactgccccc acacattctc tgggccaccg ggttgaaatc
     2521 tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag agtccgccac
     2581 acccgaaagt tccggaggga gtagcggcgg gtctagcaag ctggagaagt ttacaaactg
     2641 ctactccctg tctaagaccc tgaggttcaa ggccatccct gtgggcaaga cccaggagaa
     2701 catcgacaat aagcggctgc tggtggagga cgagaagaga gccgaggatt ataagggcgt
     2761 gaagaagctg ctggatcgct actatctgtc ttttatcaac gacgtgctgc acagcatcaa
     2821 gctgaagaat ctgaacaatt acatcagcct gttccggaag aaaaccagaa ccgagaagga
     2881 gaataaggag ctggagaacc tggagatcaa tctgcggaag gagatcgcca aggccttcaa
     2941 gggcaacgag ggctacaagt ccctgtttaa gaaggatatc atcgagacaa tcctgccaga
     3001 gttcctggac gataaggacg agatcgccct ggtgaacagc ttcaatggct ttaccacagc
     3061 cttcaccggc ttctttgata acagagagaa tatgttttcc gaggaggcca agagcacatc
     3121 catcgccttc aggtgtatca acgagaatct gacccgctac atctctaata tggacatctt
     3181 cgagaaggtg gacgccatct ttgataagca cgaggtgcag gagatcaagg agaagatcct
     3241 gaacagcgac tatgatgtgg aggatttctt tgagggcgag ttctttaact ttgtgctgac
     3301 acaggagggc atcgacgtgt ataacgccat catcggcggc ttcgtgaccg agagcggcga
     3361 gaagatcaag ggcctgaacg agtacatcaa cctgtataat cagaaaacca agcagaagct
     3421 gcctaagttt aagccactgt ataagcaggt gctgagcgat cgggagtctc tgagcttcta
     3481 cggcgagggc tatacatccg atgaggaggt gctggaggtg tttagaaaca ccctgaacaa
     3541 gaacagcgag atcttcagct ccatcaagaa gctggagaag ctgttcaaga attttgacga
     3601 gtactctagc gccggcatct ttgtgaagaa cggccccgcc atcagcacaa tctccaagga
     3661 tatcttcggc gagtggaacg tgatccggga caagtggaat gccgagtatg acgatatcca
     3721 cctgaagaag aaggccgtgg tgaccgagaa gtacgaggac gatcggagaa agtccttcaa
     3781 gaagatcggc tccttttctc tggagcagct gcaggagtac gccgacgccg atctgtctgt
     3841 ggtggagaag ctgaaggaga tcatcatcca gaaggtggat gagatctaca aggtgtatgg
     3901 ctcctctgag aagctgttcg acgccgattt tgtgctggag aagagcctga agaagaacga
     3961 cgccgtggtg gccatcatga aggacctgct ggattctgtg aagagcttcg agaattacat
     4021 caaggccttc tttggcgagg gcaaggagac aaacagggac gagtccttct atggcgattt
     4081 tgtgctggcc tacgacatcc tgctgaaggt ggaccacatc tacgatgcca tccgcaatta
     4141 tgtgacccag aagccctact ctaaggataa gttcaagctg tattttcaga accctcagtt
     4201 catgggcggc tgggacaagg ataaggagac agactatcgg gccaccatcc tgagatacgg
     4261 ctccaagtac tatctggcca tcatggataa gaagtacgcc aagtgcctgc agaagatcga
     4321 caaggacgat gtgaacggca attacgagaa gatcaactat aagctgctgc ccggccctaa
     4381 taagatgctg ccaaaggtgt tcttttctaa gaagtggatg gcctactata accccagcga
     4441 ggacatccag aagatctaca agaatggcac attcaagaag ggcgatatgt ttaacctgaa
     4501 tgactgtcac aagctgatcg acttctttaa ggatagcatc tcccggtatc caaagtggtc
     4561 caatgcctac gatttcaact tttctgagac agagaagtat aaggacatcg ccggctttta
     4621 cagagaggtg gaggagcagg gctataaggt gagcttcgag tctgccagca agaaggaggt
     4681 ggataagctg gtggaggagg gcaagctgta tatgttccag atctataaca aggacttttc
     4741 cgataagtct cacggcacac ccaatctgca caccatgtac ttcaagctgc tgtttgacga
     4801 gaacaatcac ggacagatca ggctgagcgg aggagcagag ctgttcatga ggcgcgcctc
     4861 cctgaagaag gaggagctgg tggtgcaccc agccaactcc cctatcgcca acaagaatcc
     4921 agataatccc aagaaaacca caaccctgtc ctacgacgtg tataaggata agaggttttc
     4981 tgaggaccag tacgagctgc acatcccaat cgccatcaat aagtgcccca agaacatctt
     5041 caagatcaat acagaggtgc gcgtgctgct gaagcacgac gataacccct atgtgatcgg
     5101 catcgccagg ggcgagcgca atctgctgta tatcgtggtg gtggacggca agggcaacat
     5161 cgtggagcag tattccctga acgagatcat caacaacttc aacggcatca ggatcaagac
     5221 agattaccac tctctgctgg acaagaagga gaaggagagg ttcgaggccc gccagaactg
     5281 gacctccatc gagaatatca aggagctgaa ggccggctat atctctcagg tggtgcacaa
     5341 gatctgcgag ctggtggaga agtacgatgc cgtgatcgcc ctggaggacc tgaactctgg
     5401 ctttaagaat agccgcgtga aggtggagaa gcaggtgtat cagaagttcg agaagatgct
     5461 gatcgataag ctgaactaca tggtggacaa gaagtctaat ccttgtgcaa caggcggcgc
     5521 cctgaagggc tatcagatca ccaataagtt cgagagcttt aagtccatgt ctacccagaa
     5581 cggcttcatc ttttacatcc ctgcctggct gacatccaag atcgatccat ctaccggctt
     5641 tgtgaacctg ctgaaaacca agtataccag catcgccgat tccaagaagt tcatcagctc
     5701 ctttgacagg atcatgtacg tgcccgagga ggatctgttc gagtttgccc tggactataa
     5761 gaacttctct cgcacagacg ccgattacat caagaagtgg aagctgtact cctacggcaa
     5821 ccggatcaga atcttccgga atcctaagaa gaacaacgtg ttcgactggg aggaggtgtg
     5881 cctgaccagc gcctataagg agctgttcaa caagtacggc atcaattatc agcagggcga
     5941 tatcagagcc ctgctgtgcg agcagtccga caaggccttc tactctagct ttatggccct
     6001 gatgagcctg atgctgcaga tgcggaacag catcacaggc cgcaccgacg tggattttct
     6061 gatcagccct gtgaagaact ccgacggcat cttctacgat agccggaact atgaggccca
     6121 ggagaatgcc atcctgccaa agaacgccga cgccaatggc gcctataaca tcgccagaaa
     6181 ggtgctgtgg gccatcggcc agttcaagaa ggccgaggac gagaagctgg ataaggtgaa
     6241 gatcgccatc tctaacaagg agtggctgga gtacgcccag accagcgtga agcactctgg
     6301 tggttctgga ggatctggtg gttctactaa tctgtcagat attattgaaa aggagaccgg
     6361 taagcaactg gttatccagg aatccatcct catgctccca gaggaggtgg aagaagtcat
     6421 tgggaacaag ccggaaagcg atatactcgt gcacaccgcc tacgacgaga gcaccgacga
     6481 gaatgtcatg cttctgacta gcgacgcccc tgaatacaag ccttgggctc tggtcataca
     6541 ggatagcaac ggtgagaaca agattaagat gctctctggt ggttctccca agaagaagag
     6601 gaaagtctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     6661 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     6721 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     6781 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     6841 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
     6901 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
     6961 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     7021 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     7081 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
     7141 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7201 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7261 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7321 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     7381 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7441 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7501 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     7561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7681 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8101 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9661 aaagtgccac ctg
//
