LOCUS       sequence-215579-        1751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK112
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     216..235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(532..566)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(637..1296)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1211..1230
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1478..1751,1..315)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
       61 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      421 tctcaaacgc atatacaagt ttattcttga cactagtcgg ccaaaatgat ataatacctg
      481 agtactgtta tacagaaaca gaggagatat tacatatgtg agaccagacc aataaaaaac
      541 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      601 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      661 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      721 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      781 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      841 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
      901 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      961 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1021 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1081 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1141 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1201 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1261 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     1321 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1381 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     1441 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1501 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1561 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1621 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1681 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1741 ttaccgggtt g
//
